Development of Simple Denitrification System With Autotrophic Bacteria for Developing Countries

为发展中国家开发简单的自养细菌反硝化系统

基本信息

批准号：
11480151
负责人：
HANO Tadashi
金额：
$ 4.16万
依托单位：
Oita University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (B).
财政年份：
1999
资助国家：
日本
起止时间：
1999 至 2000
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-11480151/
关键词：
Developing Countries Simplification Denitrification Autotrophic Bacteria Sulfur Thiobacuius denitrificans

项目摘要

A simple denitrification process suitable for developing countries was proposed by employing autotrophic Thiobacillus dentrificans. The effect of nitrate concentration, phosphorus concentration, alkalinity and inhibitants on the rate of denitrification was investigated with a column packed with sulfur and limestone particles involving denitrification bactaeria, and the following results were obtained.1) The denitrification proceeded without substrate inhibition up to 1,000 g-nitrate-N/m^3, which demonstrated that most of the wastewater cold be treated without dilution.2) The minimum phosphorus concentration was evaluated under constant pH controlled by borate-buffer showing that 1 mg-P/m^3 was enough for the present process. However, higher phosphorus concentration was necessary when borate buffer was not used. This was because pH at the reaction site, probably the boundary between biofilm and surface of sulfur particles, may drop due to the release of proton as a result of denitrification.3) The minimum alkalinity was found as 600g-CaCO_3/m^3 for load of 420g-N/(m^3/d). The inhibition with fluoride and Ni was not observed until 90 and 3 g/m^3, respectively.It was concluded that the present system was suitable for developing countries because it was simple without any special instruments or technical care and it offered stable performance with slight request for phosphorus even in concentrated wastewater.

提出了一种适用于发展中国家的简单反硝化工艺，采用自养牙根硫杆菌。在装有硫和石灰石颗粒的柱子上，利用反硝化细菌，研究了硝酸盐浓度、磷浓度、碱度和抑制剂对反硝化速率的影响，得到了以下结果。 1）在没有底物抑制的情况下，反硝化进行到1,000 g-硝酸盐-N/m^3，这表明大多数废水无需稀释即可进行冷处理。2）在硼酸盐缓冲液控制的恒定pH下评估最低磷浓度，结果表明1 mg-P/m^3 对于目前的过程来说已经足够了。然而，当不使用硼酸盐缓冲液时，需要更高的磷浓度。这是因为反应位点（可能是生物膜和硫颗粒表面之间的边界）的 pH 值可能会由于反硝化作用释放质子而下降。3) 最低碱度为 600g-CaCO_3/m^3负载为 420g-N/(m^3/d)。氟化物和镍分别在90和3 g/m^3时才观察到抑制作用。结论是，本系统适合发展中国家，因为它简单，不需要任何特殊仪器或技术护理，并且性能稳定，即使在浓缩废水中，对磷的要求也很低。