Analysis of the mechanism of inhibition of liver metastasis from colon cancer ------The role of Interleukin 2 receptor of the liver sinusoidal mononuclear cells

抑制结肠癌肝转移的机制分析------白细胞介素2受体对肝窦单个核细胞的作用

基本信息

批准号：
08457337
负责人：
NAKAMURA Yoshiaki
金额：
$ 4.86万
依托单位：
TAZUKE KOFUKAI MEDICAL RESEARCH INSTITUTE.
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (B)
财政年份：
1996
资助国家：
日本
起止时间：
1996 至无数据
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-08457337/
关键词：
colon cancer liver metastasis liver sinusoidal monounclear cell CD3 CD56 IL-2 receptor immuno-chemotherapy CEA

项目摘要

[Purpose] in this study, we studied the characters of SMC and PBMC,based on the hypothesis that the immune system managed by sinusoidal mononuclear cells (SMC) was damaged in the patient bearing metastatic liver cancer (MLC).[Materials and methods] We analyzed the surface antigens on SMC and PBMC by flowcytometry in seven liver specimens taken from hepatectomized patients with MLC.The patients were aged from 35 to 80,4males and 3 females. Primary lesions were cecum (2), ascending colon (2) and 3 sigmoid colon (3). In the control group, we analized PBMC of healthy candidates. Response against IL-2 was assesed by MTT assay.[Results] In MLC patients, CD3 (+) T cell decreased to 30 % (50-60% : normal) in PMBC and 40% (50% : normal) in SMC.CD16 (+) NK cells increased to 25-69% (20% : normal). Abnormal CD4/CD8 ratio in PBMC were observed in three cases, who showed high CEA value.CD8 and CD56 double positive cells were decresed below 30% in comparison with normal SMC (50%). IL-2R alpha chain was induced, but IL-2R beta chain was not recognized even after IL-2 stimulation.[Conclusion] In MLC,CTL cells were decreased in SMC,especially in the high CEA value patients, showng the possibility of lower immunological defense ability than normal. The lower responsiveness to IL-2 in CTL was also recognized. It may be effective that the immuno-chemothrapy by injection via hepatic artery or portal vein using anti-cancer drugs and additional IL-2. This therapy will increase the CTL cells in SMC and make a good control in IL-2 R system, affecting the excellent results for the therapy in liver metastasis patients.

[目的]在这项研究中，我们根据以下假设研究了SMC和PBMC的特征：在患者携带转移性肝癌（MLC）的患者中损坏了由正弦曲线单核细胞（SMC）管理的免疫系统。 MLC。患者年龄在35至80,4males和3个女性中。原发性病变为盲肠（2），升到结肠（2）和3个乙状结肠（3）。在对照组中，我们对健康候选者进行了分析。通过MTT分析对IL-2的反应。[结果]在MLC患者中，CD3（+）T细胞在PMBC中降低至30％（50-60％：正常），在SMC.CD16（+）NK细胞中40％（50％：正常）增加到25-69％（20％：正常）。在三种情况下，观察到PBMC中的CD4/CD8比率异常，它们显示出很高的CEA值。与正常SMC相比，CD8和CD56双阳性细胞脱离30％（50％）。诱导IL-2Rα链，但是即使在IL-2刺激后也无法识别IL-2Rβ链。[结论]在MLC中，SMC中的CTL细胞降低，尤其是在高CEA值患者中，表明与正常情况下免疫防御能力的可能性更低。还认识到CTL中对IL-2的响应性较低。使用抗癌药和其他IL-2通过肝动脉或门静脉注射通过肝动脉或门静脉进行免疫化疗可能是有效的。这种疗法将增加SMC中的CTL细胞，并在IL-2 R系统中良好控制，从而影响肝转移患者的疗法的出色结果。