Neurogenesis and organogenesis of zebrafish and medaka

斑马鱼和青鳉的神经发生和器官发生

• 批准号: 08308040
• 负责人: MIKI Naomasa
• 金额: $ 18.43万
• 依托单位: Osaka University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (A)
• 财政年份: 1996
• 资助国家: 日本
• 起止时间: 1996 至 1998
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-08308040/
• 关键词: zebrafish; medaka; neurogenesis; growth factor; transcription factor; nuclear transplantation; mutation; ゼブラフィシュ; Zebrafish; development; BMP-4; islet-2; medaka; transgenic; protocadherin; 神経機能; 発生・分化; 胚操作; 遺伝子導入

Mishina, M.(Sch. Med. Univ. Tokyo) found that trimethylpsoralen (TMP) produced zebrafish mutants in high efficiency and analyzed a edawakare gene which produces enhancement of branching of sensory neurons.Wakamatsu, Y.(Biosci Center, Nagoya Univ.) produced nuclear-transplanted Medaka by transplantation of nuclei from embryonic cells to unfertilized eggs with nuclei and found that formation of the embryonic body with donar gene was 32% and 4% of them normally developed.Nakayasu, H.(Biology, Okayama Univ.) established the explant culture of zebrafish brains by using immunohistochemical markers.Okamoto, H.(Brain sci. Inst. RIKEN) found that Islet-2 control axaonal pathfinding of both sensory and motor neurons in embryonic zebrafish by overexpressing LIM/homeodomain protein.Miki, N.(Med. Sch. Osaka Univ.9 isolated a novel zfh family transcription factor and found that it is involved in the determination of dorsoventral axis by overexpressing it.Ueno, N.(Nat. Inst. Basic. Biol.) found that BMP is involved in the ectodermal pattern formation ; it induces the epidermis in the ventral side and also suppresses the neural development.Takeda, H.(Biol. Sci. Nagoya Univ.) found that two pathways are involved in the neural induction in the zebrafish : one is organizer factors which binds to BMP, and the other is FGF which is involved in postrior neural induction and competes with BMP.Yamagata, K.(Tokyo Metropolitan Inst. Neurosci.) found a novel immediate early gene, arcadlin which is induced by LTP or electric shock. It has a homology with cadherin and has a cell adhesion activity, suggesting the involvement of synaptic plasticity.

Mishina, M.（东京医学大学医学院）发现三甲基补骨脂素 (TMP) 能够高效产生斑马鱼突变体，并分析了增强感觉神经元分支的 edawakare 基因。 Wakamatsu, Y.（名古屋大学生物科学中心） ）通过将胚胎细胞的细胞核移植到有核的未受精卵中，产生了核移植青鳉，并发现带有供体基因的胚胎体的形成其中 32% 和 4% 正常发育。Nakayasu, H.（冈山大学生物学）利用免疫组织化学标记建立了斑马鱼大脑的外植体培养物。Okamoto, H.（Brain sci. Inst. RIKEN）发现胰岛- 2 通过过表达 LIM/同源域蛋白来控制胚胎斑马鱼感觉和运动神经元的轴突寻路。Miki， N.(Med. Sch. Osaka Univ.9 分离出一种新的 zfh 家族转录因子，并发现它通过过度表达参与背腹轴的确定。Ueno, N.(Nat. Inst. Basic. Biol.) 发现BMP参与外胚层模式的形成； Takeda, H.(Biol. Sci. Nagoya Univ.)发现斑马鱼的神经诱导有两种途径：一种是与BMP结合的组织因子，另一个是FGF，它参与后神经诱导并与BMP竞争。Yamagata, K.(Tokyo Metropolitan Inst. Neurosci.)发现了一种新的立即早期基因，arcadlin，由 LTP 或电击诱导。它与钙粘蛋白具有同源性，并具有细胞粘附活性，表明与突触可塑性有关。

项目成果

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Izsvak, Z.、Ivics, Z. 等人：“硬骨鱼中的短反向重复转座元件及其对其扩增机制的影响。”

O.Saitoh, Y.Kubo, et al.: "RGS7 and RGS8 differentially accelerate G-protein-mediated modulation of K+ currents." J.Biol.Chem.(in press).

O.Saitoh、Y.Kubo 等人：“RGS7 和 RGS8 不同地加速 G 蛋白介导的 K 电流调节。”

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S.Koshida、M. Shinya 等人：“斑马鱼中枢神经系统的初始前后模式是由外胚层的差异能力决定的”发展。

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Kikuchi, Y.、Sekawa, H.、Okamoto, H. 等人：“胰岛 3 LIM/同源域蛋白 18・3 的 LIM 结构域过度表达导致斑马鱼的眼部和小脑缺陷。” -382 (1997)

Tsukamoto,Y., Taira,E., Miki,N., Sakuma,S.他: "Gicerin,a cell adhesion molecule,participates in the histogenesis of retina." J.Neurobiol.,. 33. 769-780 (1997)

Tsukamoto, Y.、Taira, E.、Miki, N.、Sakuma, S. 等人：“Gicerin，一种细胞粘附分子，参与视网膜的组织发生。”33. 769-780 (1997)