Function of a novel calcium-binding protein, visinin.

一种新型钙结合蛋白维西宁的功能。

• 批准号: 02454135
• 负责人: MIKI Naomasa
• 金额: $ 3.78万
• 依托单位: Osaka University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (B)
• 财政年份: 1990
• 资助国家: 日本
• 起止时间: 1990 至 1991
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-02454135/
• 关键词: visinin; cDNA Cloning; retina; pineal; light; calcium; guanylate cyclase; グアニレ-トサイラ-ゼ; 視細胞; カルシウム結合蛋白; 錐体細胞

We reported in 1985 that visinin is 24kDa and a cone specific protein. We have isolated visinin cDNA and characterized the encoded protein. We also examined the effect of visinin and anti-visinin serum on guanylate cyclase activity from frog rod outer segments.1. Visinin cDNA was isolated from chick retinal lambdagtll cDNA library, using anti-visinin serum. The nucleotide sequence revealed that visinin has three E-F hand structure and is a calcium binding protein. Visinin protein expressed in E. Coli exhibited Ca binding activity.2. Western blot and immunohistochemistry using an epitope-selected visinin antibody demonstrated that visinin exist only in the retinal cone and pinealocytes in chick.3. Visinin isolated from E. coli in which visinin was expressed and anti-visinin serum did not affect the activity of guanylate cyclase from frog r6d outer segments.4. The amounts of visinin in the pineal organ culture induced 4-8 folds in the light, but not in the dark.5. These data suggest that visinin may be involved in phototransduction in the retinal cone cell and pinealocytes through calcium metabolism.

我们于 1985 年报道了维西宁 (visinin)，分子量为 24kDa，是一种锥体特异性蛋白。我们分离了维西宁 cDNA 并表征了编码的蛋白质。我们还检测了visinin和抗visinin血清对蛙杆外节鸟苷酸环化酶活性的影响。 1．使用抗维西宁血清从鸡视网膜lambdagtll cDNA文库中分离维西宁cDNA。核苷酸序列显示visinin具有三E-F手结构，是一种钙结合蛋白。大肠杆菌表达的Visinin蛋白具有Ca结合活性。 2．采用表位选择的visinin抗体进行Western blot和免疫组化实验，结果表明visinin仅存在于鸡的视网膜锥体和松果体细胞中。 3．从表达visinin的大肠杆菌中分离得到visinin，抗visinin血清不影响青蛙r6d外节鸟苷酸环化酶的活性。 4．松果体培养物中visinin的量在光照下诱导4-8倍，但在黑暗下则不诱导。5．这些数据表明维西宁可能通过钙代谢参与视网膜锥细胞和松果体细胞的光转导。

项目成果

郭 哲輝,山形 要人,三木 直正: "網膜視細胞の光情報処理系と特異蛋白質" 生化、. 62. 1-17 (1990)

Tetsukiuo、Yoto Yamagata、Naomasa Miki：“光学信息处理系统和视网膜感光细胞的特定蛋白质” Seikan，62. 1-17 (1990)

K.Goto,K.Yamagata,N.Miki and H.Kondo,: "Direct photosensitivity of chiek pinealocytes as demonstrated by visinin-immunoreactivity." Cell Tissue Res.262. 501-505 (1990)

K.Goto、K.Yamagata、N.Miki 和 H.Kondo，：“通过粘蛋白免疫反应性证明了 chiek 松果体细胞的直接光敏性。”

三木 直正、: "色覚異常の分子機構、" 日眼会誌、. 95. 1196-1205 (1991)

Naomasa Miki：“色盲的分子机制”，日本眼科学会杂志，95. 1196-1205 (1991)

K. Yamagata, K. Goto. C-H. Kuo, H. Kondo and N. MIKI.: "Visinin ; A novel calcium-binding protein expressed in retinal cone cells." Neuron. 2. 469-476 (1990)

K.山形，K.后藤。

K.Yamagata,K.Goto,C-H.Kuo,H.Kondo and N,Miki,: "Visinin; A novel calcium-binding protein expressed in retinal cone cells." Neuron,. 2. 469-476 (1990)

K.Yamagata、K.Goto、C-H.Kuo、H.Kondo 和 N,Miki，：“Visinin；一种在视网膜锥细胞中表达的新型钙结合蛋白。”