Roles of the MFH-1 gene and WT 1 gene in kidney development

MFH-1 基因和 WT 1 基因在肾脏发育中的作用

基本信息

批准号：
08044238
负责人：
MIURA Naoyuki
金额：
$ 1.02万
依托单位：
Akita university School of Medicine
依托单位国家：
日本
项目类别：
Grant-in-Aid for international Scientific Research
财政年份：
1996
资助国家：
日本
起止时间：
1996 至无数据
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-08044238/
关键词：
MFH-1 gene Knockout mice Kidney development monoclonal antibody MFH-1 protein

项目摘要

We isolated the MFH-1 gene as one of the forkhead gene family in 1993. The family are growing and more than 20 genes are known in mice. The MFH-1 gene is expressed at the somitic mesoderm and later expressed strongly in the sclerotome. It is also expressed in the developing metanephros and later on in the glomerulus after birth.We made knockout mice of the MFH-1 gene by homologous recombination in the ES cells. First, we cloned the mouse genomic MFH-1 gene and determined its genomic structure. We found that it has no introns. We made a targeting vector by replacing the exon with neo-resistance cassette and introduced it into ES cells by electroporation. Out of 480 neomycin- and gancyclovir-resistant clones, 6 were identified to be homologous recombinants. We made chimera mice by injecting them into a blastocyst and obtained heterozygotic mice by mating chimera mice and wild B6 mice. The heterozygotes were asymptomatic. However, homozygotes were dead within ten minutes after birth.Homozygotes had abnormalities in several tissues. Here we focused the phenotype of kidneys. The size of kidneys in homozygotes was half that of the wild-type. Histologically the glomerulus, renal tubular cells and blood vesssels were present normally. But dilated renal calces were seen in homozygotes.Next, we cloned the human genomic MFH-1 gene and determined its nucleotide sequence. We found that human MFH-1 protein is 501 amino acids long and the amino acid sequence of the forkheah domain is identical in human and mouse. Identity of the mouse and human MFH-1 protein is 94%.We also made monoclonal antibodies against the human MFH-1 protein. Using thses antibodies, we found that the MFH-1 protein is expressed in chondrosarcoma, osteosarcoma and Wilms tumor cell lines.

我们将MFH-1基因分离为1993年的叉状基因家族之一。该家族正在增长，并且在小鼠中已知20多个基因。 MFH-1基因在躯体中胚层表达，后来在硬核小体中强烈表达。它也在发育中的元源中表达，后来在出生后的肾小球中表达。我们通过ES细胞中的同源重组制作了MFH-1基因的敲除小鼠。首先，我们克隆了小鼠基因组MFH-1基因并确定其基因组结构。我们发现它没有内含子。我们通过用新耐药盒代替外显子来制作靶向载体，并通过电穿孔将其引入ES细胞中。在480个新霉素和gancyclovir的克隆中，有6个被确定为同源重组。我们通过将它们注射到胚泡中，并通过配合嵌合体小鼠和野生B6小鼠获得杂合小鼠，从而制造了嵌合体小鼠。杂合子无症状。然而，纯合子在出生后十分钟内死亡。多种合物在几个组织中有异常。在这里，我们集中了肾脏的表型。纯合子中肾脏的大小是野生型的一半。从组织学上讲，肾小球，肾小管细胞和血管正常存在。但是在纯合子中看到了扩张的肾钙。我们克隆了人类基因组MFH-1基因并确定其核苷酸序列。我们发现，人MFH-1蛋白的长度为501氨基酸，并且在人和小鼠中，Forkheah结构域的氨基酸序列相同。小鼠和人类MFH-1蛋白的身份为94％。我们还对人MFH-1蛋白产生了单克隆抗体。使用THSES抗体，我们发现MFH-1蛋白在软骨肉瘤，骨肉瘤和Wilms肿瘤细胞系中表达。