Generation of the HCV-infectable mouse-an animal model for inflammation cancer

HCV感染小鼠的产生——炎症性癌症动物模型

• 批准号: 24659603
• 负责人: MIURA Naoyuki
• 金额: $ 2.41万
• 依托单位: Hamamatsu University School of Medicine
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Challenging Exploratory Research
• 财政年份: 2012
• 资助国家: 日本
• 起止时间: 2012-04-01 至 2014-03-31
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-24659603/
• 关键词: C型肝炎ウイルス; 複製因子; 肝細胞癌; 慢性炎症; 複製; 侵入; ウイルス粒子形成; ウイルス粒子; ゲノムRNA; 感染

Experimental plan expected that short promoter has no transcriptional activity to synthesize HCV genome RNA. Unexpectedly, the transgenic mouse showed that they produce HCV genome RNA in hepatocytes and also viral particles into serum. Using the mouse serum from the transgenic mouse, the virus infected Huh7.5.1 cells and produced Core protein in the cytoplasm. This means that the virus was infectious.Next we investigated whether the HCV genome RNA can replicate in the mouse hepatocytes. When the replication inhibitor was injected into the abdomen of the mouse, the RNA copy number was decreased by 30% in the hepatocytes and serum. This result showed that mouse replication factor(s) were competent for viral RNA replication as human factor(s) do.

实验计划预期短启动子不具有合成HCV基因组RNA的转录活性。出乎意料的是，转基因小鼠显示它们在肝细胞中产生HCV基因组RNA，并且还在血清中产生病毒颗粒。使用来自转基因小鼠的小鼠血清，病毒感染Huh7.5.1细胞并在细胞质中产生Core蛋白。这意味着该病毒具有传染性。接下来我们研究了HCV基因组RNA是否可以在小鼠肝细胞中复制。当将复制抑制剂注射到小鼠腹部时，肝细胞和血清中的RNA拷贝数减少了30%。该结果表明，小鼠复制因子能够像人类因子一样进行病毒 RNA 复制。

项目成果

Phosphorylation Regulates FOXC2-Mediated Transcription in Lymphatic Endothelial Cells

• DOI: 10.1128/mcb.01387-12
• 发表时间: 2013-10-01
• 期刊: MOLECULAR AND CELLULAR BIOLOGY
• 影响因子: 5.3
• 作者: Ivanov, Konstantin I.;Agalarov, Yan;Petrova, Tatiana V.
• 通讯作者: Petrova, Tatiana V.

FOXC2 expression links epithelial-mesenchymal transition and stem cell properties in breast cancer.

• DOI: 10.1158/0008-5472.can-12-2962
• 发表时间: 2013-03-15
• 期刊: Cancer research
• 影响因子: 11.2
• 作者: Hollier BG;Tinnirello AA;Werden SJ;Evans KW;Taube JH;Sarkar TR;Sphyris N;Shariati M;Kumar SV;Battula VL;Herschkowitz JI;Guerra R;Chang JT;Miura N;Rosen JM;Mani SA
• 通讯作者: Mani SA

The regulation of endogenous retinoic acid level through CYP26B1 is reqired for elevation of palatal shelves

腭架抬高需要通过 CYP26B1 调节内源性视黄酸水平

• 发表时间: 2012
• 期刊: Dev Dyn
• 作者: Okano J;Kimura W;Papaionnou VE;Miura N;Yamada G;Shiota K;Sakai Y
• 通讯作者: Sakai Y

TGFα, c-MYC, mutated CTNNB1 and their combinations act distinctly on the Hep3B tumors in nude mice

TGFα、c-MYC、突变CTNNB1及其组合对裸鼠Hep3B肿瘤有明显作用

• 发表时间: 2014
• 作者: Noritake H;Amin M;Kobayashi Y;Miura N
• 通讯作者: Miura N

FOXC2 expression links epitherial-mesenchymal transition and stem cell properties in breast cancer

FOXC2 表达将乳腺癌的上皮间质转化和干细胞特性联系起来

• 发表时间: 2013
• 期刊: Cancer Res
• 影响因子: 11.2
• 作者: Hollier BG;Tinnirello AA;Werden SJ;Evans KW;Taube JH;Sarker TR;Sphyris N;Shariati M;Kumar SV;Battula VL;Herschkowitz JI;Guerra R;Chang JT;Miura N;Rosen JM;Mani SA
• 通讯作者: Mani SA