Mechanisms of absorption, transport, and storge of vitamin A-by using rats and polar animals-

维生素A的吸收、运输和储存机制-利用大鼠和极地动物-

基本信息

批准号：
08044237
负责人：
SENOO Haruki
金额：
$ 1.34万
依托单位：
Akita university School of Medicine
依托单位国家：
日本
项目类别：
Grant-in-Aid for international Scientific Research
财政年份：
1996
资助国家：
日本
起止时间：
1996 至无数据
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-08044237/
关键词：
Svalbard archipelago Arctic animal vitamin A hepatic stellate cell hypervitaminosis A leukemin cancer therapy retionid-binding proteins

项目摘要

Seven Svalbard reindeers, 3 ringed seals, 2 bearded seals, 4 glaucous gulls, 3 Brunnich's guillemots, and 1 puffin were caught in the Svalbard archipelago from August through September, 1996. Fresh each organs, namely, the liver, kidneys, lungs, jejunum, spleen, and skin were investigated with gold chloride staining, fluorescence microscopy for autofluorescence of vitamin A,Sudan 3 lipid staining, hematoxylin and eosin staining, Azan staining, Ishii and Ishii's silver impregnation method, and transmission electron microscopy.The Arctic animals and birds store a large amount of vitamin A in hepatic stellate cells. Only a small amount of vitamin A existed in other organs : kidneys, jejunum, spleen, lungs, and skin. On the other hand, extrahepatic stellate cells in normal rats were demonstrated to store vitamin A in the kidneys, lungs, spleen, and intestine.These results indicated that the Arctic animals and birds can store a huge amount of vitamin A in hepatic stellate cells in physiological conditions. These data also indicated that the molecular mechanisms such as retinoid-binding proteins in the Arctic animals and birds play pivotal roles in their vitamin A storage. Applying these molecular mechanisms the prevention methods will be developed for hypervitaminosis A as a side effect of vitamin A treatment to malignant diseases such as leukemia.

Seven Svalbard reindeers, 3 ringed seals, 2 bearded seals, 4 glaucous gulls, 3 Brunnich's guillemots, and 1 puffin were caught in the Svalbard archipelago from August through September, 1996. Fresh each organs, namely, the liver, kidneys, lungs, jejunum, spleen, and skin were investigated with gold chloride staining,荧光显微镜，用于维生素A，苏丹3脂质染色，苏木精和曙红染色的自动荧光，Azan染色，ISHII和ISHII的银色浸渍方法以及透射电子显微镜。其他器官中只有少量的维生素A：肾脏，空肠，脾脏，肺和皮肤。另一方面，正常大鼠中肝外星状细胞被证明可以将维生素A储存在肾脏，肺，脾和肠中。这些结果表明，北极动物和鸟类可以在生理条件下将大量的维生素A存储在肝星细胞中。这些数据还表明，北极动物和鸟类中类视黄素结合蛋白等分子机制在其维生素A储存中起关键作用。采用这些分子机制，将开发用于高乙酰胺病A作为维生素A治疗对恶性疾病（例如白血病）的副作用的预防方法。