Mechanisms of absorption, transport, and storage of vitamin A-by using rats and polar animals-

维生素A的吸收、运输和储存机制-利用大鼠和极地动物-

• 批准号: 09044251
• 负责人: SENOO Haruki
• 金额: $ 4.42万
• 依托单位: Akita University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for international Scientific Research
• 财政年份: 1997
• 资助国家: 日本
• 起止时间: 1997 至 1998
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-09044251/
• 关键词: vitamin A; Arctic animals; hepatic stellate cells; retinyl ester; Svalbard archipelago; hypervitaminosis A; food web; hepatic fibrosis; レエチニルエステル; スバルバ-ル群島; 自家蛍光; 塩化金法

Two polar bears, 8 Arctic foxes, 6 bearded seals, 22 glaucous gulls, 5 fulmars, 4 Bruenich's guillemots, 6 ringed seals, 5 hooded seals, 6 puffins, and 7 Svalbard reindeers were caught in the Svalbard archipelago (situated at lat. 80 N). Fresh organs, namely, the liver, kidney, spleen, lung, and jejunum were investigated with gold chloride staining, fluorescence microscopy for autofluorescence of vitamin A, hematoxylin and eosin staining, azan staining, Ishii-Ishii's silver impregnation, transmission electron microscopy and high-performance liquid chromatography.The mammals and birds stored a large amount of vitamin A in the liver [23,300 (polar bear, n=2), 18,439*2,314 (Arctic fox, n=8), 5956*5857 (bearded seal, n=6), 1,551*2,486 (ringed seal, n=6), 921*264 (Svalbard reindeer, n=7), 4,710*3,164 (glaucous gull, n=22), 1,589*225 (Bruenich's guiHemot, n=4), and 1183*589 (puffin, n=6) nmole retinyl ester/g wet tissue]. Very strong autofluorescence of vitamin A emanated from the non-parenchymal cells in the liver. These cells showed positive reaction with gold chloride and stained black. Electronmicroscopically these cells existed in the perisinusoidal space of Disse and contained many lipid droplets within the cytoplasm. These anamals showed no hepatic fibrosis though they are in naturally occurred hypervitaminosis A.These findings suggest that these vitamin A-storihg cells are hepatic stellate cells and the Arctic animals that exist in a higher position of the food web in the Arctic area have well-developed vitamin A-storing mechanisms in the hepatic stellate cells.

两只北极熊，8个北极狐狸，6个胡须的海豹，22个魅力的海豹，5个富尔马斯，4张布鲁尼奇的吉勒犬，6个环形密封件，5个连帽密封件，6个海雀和7个svalbard驯鹿，在Svalbard Archipelago（位于Lat。80n）中。研究了新鲜的器官，即用金氯化物染色，荧光显微镜进行肝脏，肾脏，脾脏，肺和空肠研究肝脏中维生素A的量[23,300（北极熊，n = 2），18,439*2,314（北极狐狸，n = 8），5956*5857（胡须密封，n = 6），1,551*2,486（2,486） （Glaucous Gull，n = 22），1,589*225（Bruenich's Guihemot，n = 4）和1183*589（Puffin，n = 6）Nmole视乙酰酯/G湿组织]。维生素A的非常强的自动荧光是从肝脏中非核细胞产生的。这些细胞显示与金氯化物的阳性反应，并染成黑色。电子显微镜从卵形的核苷空间中存在，并在细胞质中包含许多脂质液滴。这些解剖没有表现出肝纤维化，尽管它们自然发生了。

项目成果

Senoo H,et al.: "Molecular mechanisms in the reversible regulation of morphology,proliferation,and collagen metabolism in hepatic stellate cells by the three-dimensional structure of ECM" J Gastroent Hepatol. 13. S19-32 (1998)

Senoo H 等人：“ECM 三维结构对肝星状细胞形态、增殖和胶原代谢的可逆调节的分子机制”J Gastroent Hepatol。

Nagy NE,et al.: "Storage of vitamin A in extrahepatic stellate cells in normal rats." J Lpid Res.38. 645-658 (1997)

Nagy NE 等人：“正常大鼠肝外星状细胞中维生素 A 的储存。”

Kojima N,et al.: "Stellate cells (vitamin A-storing cells) change their cytoskeleton and signal transduction system according to extracellular matrix" Histochem Cell Biol. 110. 121-128 (1998)

Kojima N 等人：“星状细胞（维生素 A 储存细胞）根据细胞外基质改变其细胞骨架和信号转导系统”Histochem Cell Biol。

Senoo H,et al.: "Hepatic stellate cells-from viewpoint of retinoid handling and functions of extracellular matrix" Acta Anat Nippon. 72. 79-94 (1997)

Senoo H 等人：“肝星状细胞 - 从类维生素A处理和细胞外基质功能的角度来看”Acta Anat Nippon。

Senoo H.: "Vitamin A-storing system in the Arctic animals" Acta Anat. Nippon.72. 301 (1997)

Senoo H.：“北极动物的维生素 A 储存系统”Acta Anat。