EPIDEMIOLOGICAL STUDY ON LYME DISEASE IN THE FAR EASTERN COUNTRY

远东国家莱姆病流行病学研究

• 批准号: 08041181
• 负责人: MASUZAWA Toshiyuki
• 金额: $ 7.1万
• 依托单位: University of Shizuoka
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for international Scientific Research
• 财政年份: 1996
• 资助国家: 日本
• 起止时间: 1996 至 1997
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-08041181/
• 关键词: LYME DISEASE; BORRELIA; IXODES; BORRELIA BURGDORFERI; BORRELIA GARINII; BORRELIA AFZELII; SYSTEMATIC CLASSIFICATION; FAR EAST; Borrelia burgderteri; ライムボレリア症

We made Lyme disease borrelia survey of vector ticks and resevoir animals in Northern part of China and the Yangtze River valley, and Korea. Borrelia isolates from these sources and from far eastern Russia we got previously were analyzed by 5S-23S rRNA intergenic spacer and flagellin gene PCR-RFLP analysis and sequence analysis.Isolates from Ixodes persulcatus collected Northern China and far eastern Russia were identified as B.afzelii or B.garinii. B.garinii generate RFLP pattern C which is commonly found in Japan but not in Europe were dominant species in these area. Furthermore, isolates from I.nipponensis collected in central Korea were identified as B.garinii of far eastern type or B.afzelii. Isolates from I.nipponensis tentatively described as B.afzelii-related group obtained from Korean researcher were characterized on intergenic spacer and 16SrRNA sequences. These isolates showed relatively higher similarity values to B.valaisiana isolated from I.ricinus in Europe and strain Am501 from I.columnae in Japan. Isolates from Apodemus spp.and Rattus spp.captured in the Yangtze River valley were identified as B.afzelii-related group and new genotype, respectively. Also, one isolate from I.ovatus in the area showed the same feature to those from the Rattus spp. Further study to described new species is undertaken.By the phylogenetic analysis based on the sequence of outer surface protein A,B,and C,genes, transmission of B.garinii and B.afzelii between Japan and Europe via far eastern country, such as far east Russia, northern China, and Korea was suggested.

我们对中国北部和扬特河谷和韩国进行了莱姆病渗透症调查。从这些来源和俄罗斯远方的伯罗利分离株通过5S-23S RRNA基因间间隔物和鞭毛蛋白基因PCR-RFLP分析和序列分析和序列分析。 B.Garinii产生的RFLP模式C，在日本通常发现，但在欧洲却是这些地区的主要物种。此外，从韩国中部收集的i.nipponensis的分离株被确定为远东类型的b.garinii或b.afzelii。从韩国研究人员中获得的分离株，暂定地描述为与阿夫齐里相关的组，在基因间间隔和16sRRNA序列上进行了表征。这些分离株显示出与从欧洲的i.ricinus分离的b.valaisiana的相对较高的相似性值，而日本的i.columnae菌株AM501。分别是从Apodemus spp.和rattus spp。在长江河谷接收的rattus spp。分别确定为B.Afzelii相关组和新基因型。同样，该区域中的一个分离株与Rattus spp的人具有相同的特征。通过基于外表面蛋白A，B和C，基因，B.Garinii和B.Fzelii在日本和欧洲之间通过远东地区（例如东部俄罗斯，北部北部，中国北部和韩国）之间的序列，基于外表面蛋白A，B和C的序列进行系统发育分析，提出了进一步的研究。

项目成果

FEMS Immunol.Med.Microbiol.18. 249-261 (1997)

FEMS 免疫医学微生物学 18。

Toshiyuki Masuzawa, Tetsuro komikado, Atue Iwaki, Hiroyuki Suzuki, Kazuhide Kaneda, and Yasutake Yanagihara.: "Characterization of Borrelia sp.Iiolated from Ixodes tanuki, I.turdus, and I.columnae in Japan by restriction fragment length polymorphism of rr

Toshiyuki Masuzawa、Tetsuro komikado、Atue Iwaki、Hiroyuki Suzuki、Kazuhide Kaneda 和 Yasutake Yanagihara。：“通过 rr 的限制性片段长度多态性表征来自日本狸蜱、I.turdus 和 I.columnae 的疏螺旋体 sp.Iiolated”

Jianhui Wang, Toshiyuki Masuzawa, Tetsuro komikado, and Yasutake Yanagihara.: "Consensus sequence on the genes encoding the major outer surface proteins (OspA and OspB) of Borrelia garnii isolate." Microbiol.Immunol.41. 83-91 (1997)

Kenhui Wang、Toshiyuki Masuzawa、Tetsuro komikado 和 Yasutake Yanagihara：“编码卡尼疏螺旋体分离物主要外表面蛋白（OspA 和 OspB）的基因的共有序列。”

Kazuhide Kaneda, Toshiyuki Masuzawa, Kitsuzo Yasugami, Takashi Suzuki, Yasuo Suzuki, and Yasutake Yanagihara.: "Glycosphingolipid-binding protein of Borrelia burgdorferi sensu lato." Infect.Immun.65. 3180-3185 (1997)

Kazuhide Kaneda、Toshiyuki Masuzawa、Kitsuzo Yasugami、Takashi Suzuki、Yasuo Suzuki 和 Yasutake Yanagihara：“伯氏疏螺旋体的糖鞘脂结合蛋白”。

Toshiyuki Masuzawa et al.: "Genetic diversity of Borrelia burgdorferi sensu lato isolated in far eastern Russia." Microbiol.Immunol.41. 595-600 (1997)

Toshiyuki Masuzawa 等人：“俄罗斯远东地区分离出的伯氏疏螺旋体的遗传多样性。”