Design, structures and functions of stabilized artificial ion channels

稳定人工离子通道的设计、结构和功能

• 批准号: 07680629
• 负责人: IIDA Akira
• 金额: $ 1.28万
• 依托单位: KYOTO UNIVERSITY
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 1995
• 资助国家: 日本
• 起止时间: 1995 至 1996
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-07680629/
• 关键词: artificial ion channel; peptide bunndle; Trichoderma polysporum; trichosporin; peptaibol; voltage-dependent channel; imperfect fungus; barrel stave

20-residue peptaibols, trichosporins-B produced by the fungus Trichoderma polysporum, forms voltage-dependent ion channels in planar lipid bilayr membranes. To clarify primary factors for channel stabilization and to develop new functional materials such as artificial ion channels, trichosporin derivatives including a template-assembled dimer and tetramer and two disulfide-linked dimers were synthesized and their channel-forming properties were investigated.The results obtained in this study were summarized as followes ;1.The chennels induced by truncated and elongated derivertives were unstable, suggesting that peptide length fitting the membrane thickness is quite important.2.The Pro-kinked structure is not essential for voltage-dependent channel formation and very important for forming stable channels with large pore size.3.Peptide lipophilicity promotes peptide aggregation and this is not in accordance with channel stability.4.Stability of channels is influenced by the states of supermolecules consisting of pepides, lipids and water.5.All chemically assembled peptides (helix bundle) form very long-lasting channels, supporting that peptaibol channels can be explained through the barrel stave model.6.Success in formation of the stable channels may lead to developing novel functional materials.

由真菌trichoderma polysporum产生的20个残留peptaibols，形成平面脂质bilayr膜中的电压依赖性离子通道。为了阐明通道稳定的主要因素并开发新的功能材料，例如人造离子通道，合成了包括模板组装的二聚体和四聚体以及两个二硫键连接的二聚体，并研究了其通道组合的特性。在此研究中，该研究的结果是一致； suggesting that peptide length fitting the membrane thickness is quite important.2.The Pro-kinked structure is not essential for voltage-dependent channel formation and very important for forming stable channels with large pore size.3.Peptide lipophilicity promotes peptide aggregation and this is not in accordance with channel stability.4.Stability of channels is influenced by the states of supermolecules consisting of pepides, lipids and水。5。所有化学组装的肽（螺旋束）形成非常持久的通道，支持Peptaibol通道可以通过枪管座模型来解释。6.Success在形成稳定通道的过程中可能导致开发新型功能材料。

项目成果

Yasuo Nagaoka: "Ion-Channel-Forming and Carecholamine-releasing Activities of Elongated and Truncuted Aualogues of Trichosporin-B" J. Chem. Soc., Chem. Commun.1995. 2203-2204 (1995)

Yasuo Nagaoka：“丝孢菌素 B 的延长和截短类似物的离子通道形成和羧酚胺释放活性”J. Chem。

Shun-ichi Wada: "ION CHANNNEL-FORMING PROPERTY OF TRICHOROVIN-XII,AN 11-RESIDUE PEPTAIBOL FROM THE FUNGUS TRICHODERMA VIRIDE,IN PLANAR LIPID BILAYER MEMBRANES" Bioong.Med Chem.Lett.6. 2275-2278 (1996)

Shun-ichi Wada：“Trichorovin-XII 的离子通道形成特性，来自真菌绿色木霉的 11 残基 PEPTAIBOL，在平面脂质双层膜中”Bioong.Med Chem.Lett.6。

Yasuo Nagaoka: "Role of Gln^7 in the ion-channel-forming properties of the trichosporin-B-Vla" J. Chem Soc. , Chem. Commu.1996. 1079-1080 (1996)

Yasuo Nagaoka：“Gln^7 在毛孢菌素-B-Vla 离子通道形成特性中的作用”J. Chem Soc。

Shun-ichi Wada: "ION CHANNNEL-FORMING PROPERTY OF TRICHOROVIN-XII, AN II-RESIDUE PEPTAIBOL FROM THE FUNGUS TRICHODERMA VIRIDE, IN PLANAR LIPID BILAYER MEMBRANES" Bioorg. Med. Chem. Lett.6. 2275-2278 (1996)

Shun-ichi Wada：“Trichorovin-XII 的离子通道形成特性，它是来自真菌绿色木霉的 II 残留 PEPTAIBOL，在平面脂质双层膜中”Bioorg。

Yasuo Nagaoka: "Ion-channel-forming and Catecholamine-releasing Activities of Elongated and Truncated Analogues of Trichosporin-B" J.Chem Soc.,Chem.Commu.1995. 2203-2204 (1995)

Yasuo Nagaoka：“丝孢菌素-B 的延长和截短类似物的离子通道形成和儿茶酚胺释放活性”J.Chem Soc.，Chem.Commu.1995。