Metabolism of oxidative phospholipids in peroxidized human erythrocytes membranes

过氧化人红细胞膜中氧化磷脂的代谢

• 批准号: 07672376
• 负责人: NAKAGAWA Yasuhito
• 金额: $ 1.34万
• 依托单位: Kitasato University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 1995
• 资助国家: 日本
• 起止时间: 1995 至 1996
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-07672376/
• 关键词: oxidative stress; lipid peroxide; oxidative phospholipid; aldehydic phospholipid; ageing; 生成膜; 生体膜

The aim of this project was to examine the metabolism of oxidized phospholipids in biological membranes peroxidized with different kinds of radicals which were alkyl radical produced with AAPH and hydroxy radical with xanthin/xanthin oxidase. CGP hydroperoxide as a primary product of peroxidation significantly formed in RBC membrane peroxidized with AAPH.No consisitent generation of CGP hydroperoxide was evident in XO/XOD/Fe peroxidation, indicating that CGP hydroperoxide is instable in this system. Aldehydic CGP as a secondary product of hydroperoxide decomposition also detected in AAPH-peroxidized RBC membrane. AAPH mainly oxidized CGP to hydroperoxide and aldehydic phospholipids and the production of thiobarbityric acid-reactive substance (TBARS) was relatively low. On the other hand, XO/XOD/Fe peroxidation caused the potential decompositon of phospholipids to eliminate large amounts of TBARS in conrast to generate a trace amounts of oxidized phopholipids. The present study indicates that the process of peroxidation of phospholipids in biomembrane greatly depends on their strucure and the peroxidation condition.The amounts of oxidized phospholipids were also determined in various tissues of young and old rats. Phospholipid hydroperoxides and aldehydic phospholipids in liver, brain heart and kidney of old rats were significantly higher than those of young rats.The present studt demonstrated the productions of oxidazed phospholipids, especially aldehydic phospholipids in biological samples suffered with oxidative stress at the first time.

该项目的目的是检查用不同种类的自由基过氧化的生物膜中氧化磷脂的代谢，这些自由基是用Aaph产生的烷基自由基和羟基蛋白酶/Xanthin氧化酶产生的烷基自由基。 CGP氢过氧化物作为过氧化的主要产物，在用AAPH过氧化的RBC膜中显着形成。在XO/XOD/FE过氧化中，CGP氢过氧化物的同一同氧化产生，表明CGP氢过氧化物在该系统中很不起。在AAPH过敏的RBC膜中也检测到醛CGP作为氢过氧分解的次级产品。 AAPH主要将CGP氧化为氢过氧化物和醛磷脂，并产生硫巴酸反应性物质（TBARS）的产生相对较低。另一方面，XO/XOD/Fe过氧化导致磷脂的潜在分解可消除大量的TBAR，以产生痕量的氧化磷脂。本研究表明，生物膜中磷脂的过氧化过程很大程度上取决于其诱导和过氧化条件。在各种老年大鼠的组织中，还确定了氧化磷脂的量。老鼠的肝脏，脑心脏和肾脏的磷脂氢过氧化物和醛明显高于年轻大鼠的磷脂。目前的螺柱表明，在第一次氧化胁迫下，生物学样本中氧化磷脂的产生，尤其是在氧化胁迫中的氧化磷脂的产生。