Investigation of stam cells in experimental salivary gland carcinogenesis. (Immunohistochemical evaruation for oncogene product)

实验性唾液腺癌发生中干细胞的研究。

• 批准号: 07672064
• 负责人: SUMITOMO Shinichiro
• 金额: $ 1.47万
• 依托单位: Asahi University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 1995
• 资助国家: 日本
• 起止时间: 1995 至 1996
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-07672064/
• 关键词: Submandibular gland; Carcinogenesis; Immunohistochemistry; Oncogene; c-erbB-2; Cell Proliferation; PCNA; EGF; c-erbB-2; 細胞増殖; FGF; Submandibular Gland; Carcinogenesis; Immunohistochemistry; Oncogene; Cell proliferation

Proliferating cells and its immunohistochemical properties were evaluated during experimental carcinogenesis induced by DMBA containing sponge-pellet implantation and after duct-ligation of rat submandibular glands (SMGs).In the normal rat SMGs, proliferating cells were very very few, epidermal growth factor (EGF) was confined to the granules in granular convoluted tubules (GCTs), S-100 alpha was found in pillar and transition cells in GCT and striated duct (SD) and excretory duct (ED), K8.12 keratin, basic fibroblast growth factor (bFGF), and c-erbB-2 oncoprotein were found in SD and ED,and laminin existed at basement membrane of glandular epithelia and blood vessels.Two to three weeks after DMBA/sponge implantation, small clusters of proliferating cells were observed in necrotic oedematous area surrounding DMBA/sponge and duct-like structures and dilated ductal segments with small numbers of proliferating cells (10-25%) were found in inflammatory area surrounding necrotic oedematous … More area. These components showed positive for K8.12 keratin, S-100 alpha protein, bFGF,and c-erbB-2 oncoprotein and negative for EGF.Four to six week after DMBA/sponge implantation, keratinized epithelium extended surrounding DMBA/sponge like a cyst and some proliferating cells were observed in basal and parabasal area of cystic epithelium (* 27%). Histological and immunohistochemical features of cystic epithelium were similar to those of oral leucoplakias and c-erbB-2 oncoprotein was observed in this lesion. Squamous cell carcinomas (SCC) arose 8 to 12 weeks after DMBA/sponge implantation. Proliferating cells were observed mainly in basal and parabasal area of SCC and the frequency of proliferating cells were more than 30%. C-erbB-2 oncoprotein was observed also in the SCC.Invasive growth feature of tumor cells with breakages of basement membrane were some times found in after 12 weeks speciments.In the duct-ligated SMG,proliferating cells were increased in duct-like structures and the peak of the PCNA labeling was about 50% at the third day and then gradually decreased into 10% at the 21st day of experiment. Histological and immunohistochemical findings of duct-like structures in duct-ligated SMG were similar to those of duct-like structures which appeared during carcinogenesis. In unligated gland of experimental animals showed proliferation activity in acinar cells and its peak was at the second day (* 20%) and rapidly decrease into normal level at the 10th day of experiment.These results suggested that in case of acceleration of secretory function of SMG,secretory cells may proliferate as for the functional adaptation. However, in case of damage, ductal segments have more potential for proliferation and these cells may be the major participant cells during glandular regeneration and possibly source in the histogenesis of the majority of salivary gland tumors. Furthermore, apperance of c-erbB-2 oncoprotein in ductal segment and tumor cells, and EGF is accumulated in GCT,these findings may influences of rapidly carcinogenesis compared to another organs. Less

在含有赞助者 - 细胞植入的DMBA诱导的实验性癌作用中评估了增殖细胞及其免疫组织化学特性，并在大鼠下颌下网（SMGS）的导管结合后。 （GCT）在GCT和横纹管（SD）和极端导管（ED），K8.12角蛋白，基本成纤维细胞生长因子（BFGFF）和C-ERBB-2癌蛋白中发现基本成纤维细胞生长因子（BFGF），在SD和ED中发现了基底型lamin和Lamin noder的基础，在GCT和横纹管（SD）和极端导管（ED）中发现了S-100α DMBA/海绵植入后，在DMBA/SPONGE和类似导管样结构的坏死卵形区域中观察到了少量的增殖细胞，并在周围的炎症区域中发现了少量增殖细胞（10-25％），在炎症区域周围的坏死区域中发现了较少的细胞。这些成分在K8.12角蛋白，S-100α蛋白，BFGF和C-ERBB-2癌蛋白中呈阳性，而EGF.4到dmba/Sponge植入后六个星期，角膜植入，角化化的上皮扩展，如DMBA/Sponge和某些细胞，并且在某些细胞中占据了基本范围，并在某些细胞中进行了繁殖，并占据了基础上的繁殖，并占据了繁殖的范围。 （* 27％）。在此病变中观察到囊性上皮的组织学和免疫组织化学特征与口服白叶藻和C-ERBB-2癌蛋白相似。 DMBA/SPONGE植入后8至12周出现方形细胞癌（SCC）。在SCC的碱性和旁巴面积中观察到增殖细胞，增殖细胞的频率超过30％。在SCC中也观察到C-ERBB-2癌蛋白。在12周的规格后，有时发现了肿瘤细胞的肿瘤细胞的染色性生长特征。导管绑扎的SMG中管道样结构的组织学和免疫组织化学发现与癌变期间出现的管道样结构相似。在实验动物的无原始腺体中，腺泡细胞的增殖活性在第二天（* 20％），在实验的第10天迅速降低到正常水平。这些结果表明，在加速SMG的分泌功能的情况下，分泌细胞可能会随着功能适应而增殖。但是，如果损坏，导管片段具有更大的增殖潜力，这些细胞可能是腺体再生过程中的主要参与者细胞，并且可能是大多数唾液腺肿瘤组织发生的可能来源。此外，导管片段和肿瘤细胞中C-ERBB-2癌蛋白以及EGF的表现在GCT中积累，与另一个器官相比，这些发现可能会影响快速致癌作用。较少的

项目成果

Sumitomo S, et. al.: "Immunohistochemical evaluation of experimental squamous cell carcinoma in rat submandibular glands" Oral Oncology. 4B. 92-95 (1995)

住友 S 等。

S. Sumitomo, K. Hashimura, P. Shrestha, M. Mori: "Immunohistochemical evalution of experimental squamous cell carcinima in rat submandibular glands." Oral Oncology. 4B. 92-95 (1995)

S. Sumitomo、K. Hashimura、P. Shrestha、M. Mori：“大鼠颌下腺实验性鳞状细胞癌的免疫组织化学评估。”

Shinichiro SUMITOMO,Mayuko KUNIKATA- SUMITOMO,Junji HASHIMOTO,Miyako NAMBA,Prashanta SHRESTHA,Shuji KURENUMA,Neeta JAYASHINGHE,Masahiko MORI: "Cell proliferation activity in duct-ligated submandibular gland" Acta histochem cytochem. 28-1. 1-9 (1995)

Shinichiro SUMITOMO，Mayuko KUNIKATA-SUMITOMO，Junji Hashimoto，Miyako NAMBA，Prashanta SHRESTHA，Shuji KURENUMA，Neeta JAYASHINGHE，Masahiko MORI：“导管结扎下颌下腺中的细胞增殖活性”组织化学细胞化学。

S.Sumitomo,K.Hashimura,M.Mori: "Growth pattern of experimental squamous cell carcinoma in rat submandibular glands -An immunohistochemical evaluation-." Oral Oncol Eur J Cancer. 32. 97-105 (1996)

S.Sumitomo、K.Hashimura、M.Mori：“大鼠颌下腺实验性鳞状细胞癌的生长模式 - 免疫组织化学评估 -”。

S. Sumitomo, K. Hashimura, M. Mor: "Growth pattern of experimental squamous cell carcinoma in rat submandibular glands-An immunohistochemical evaluation-." Oral Oncol Eur J Cancer. 32. 97-105 (1996)

S. Sumitomo、K. Hashimura、M. Mor：“大鼠颌下腺实验性鳞状细胞癌的生长模式 - 免疫组织化学评估 -”。