Glycobiological Study of Malignant Tumors in Ophthalmology

眼科恶性肿瘤的糖生物学研究

基本信息

批准号：
07671928
负责人：
UEHARA Fumiyuki
金额：
$ 1.54万
依托单位：
Kagoshima University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
1995
资助国家：
日本
起止时间：
1995 至 1996
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-07671928/
关键词：
Tumor Glycobiology Lectin Sialyltransferase Mucin Ki 67 Keratin

项目摘要

The glycoconjugates in various malignant tumors in eyelids and orbita were analyzed by lectin histochemistry and in situ hybridization histochemistry using cRNA probes for sialiltransferases (ST). We considered that the cells, which expressed both cytoplasmic distribution of ST-mRNA and binding of lectins, specific for sialic acids, to the cell surfaces, were actively producing sialoglycans. We also considered that the cells, whose surfaces were stained with the lectins without cytoplasmic distribution of ST-mRNA,have completed the synthesis of sialoglycans. Based on these view points, this glycohistochemical technique is useful for dynamic analysis of the glycoconjugates in individual tumor cells. This technique revealed that O-linked sialoglycan, whose turnover-rate is slow, may be distributed over the cells of thickened spinocellular layr in the tumor of seborrheic keratosis and involved in its pathomechanism. We also analyzed the basal cell calcinoma in eyelids and the lacrimal pleomorphic adenoma using this method.MUC 1, one of the O-linked glycoconjugates, was immunohistochemically detected in the tumor cells of all cases of squamous cell carcinoma and sebaceous carcinoma in eyelids whereas it was absent in those of all cases of basal cell carcinoma and benigh tumors in eyelids. These findings suggested that MUC 1 may be involved in expression of malignant features including metastasis. On the other hand, the Ki 67 antigen-expression rates of malignant eyelid tumors were higher than those of benigh tumor, suggesting that Ki 67 can be used to distinguish between malignant and benigh tumors. Immunohistochemical distribution of kearatin revealed that the basal cell carcinoma in eyelids is consisted of uniform tumor cells, all of which express the same keratin, while the squamous cell carcinoma and sebaceous carcinoma are composed of various differentiatiating tumor cells.

通过凝集素组织化学和使用唾液酸转移酶（ST）cRNA探针的原位杂交组织化学分析眼睑和眼眶各种恶性肿瘤中的糖缀合物。我们认为，表达 ST-mRNA 胞质分布和与细胞表面特异性结合唾液酸的凝集素的细胞正在积极产生唾液聚糖。我们还认为，表面被凝集素染色而胞浆内没有ST-mRNA分布的细胞已经完成了唾液聚糖的合成。基于这些观点，这种糖组织化学技术可用于动态分析单个肿瘤细胞中的糖缀合物。该技术揭示了周转速度缓慢的O-连接唾液聚糖可能分布在脂溢性角化病肿瘤增厚的棘细胞层细胞上，并参与其病理机制。我们还利用该方法分析了眼睑基底细胞钙癌和泪道多形性腺瘤。在所有眼睑鳞状细胞癌和皮脂腺癌病例的肿瘤细胞中，免疫组化检测到了 O 连接糖缀合物之一 MUC 1，而在所有基底细胞癌和眼睑良性肿瘤病例中均不存在。这些发现表明 MUC 1 可能参与包括转移在内的恶性特征的表达。另一方面，眼睑恶性肿瘤的Ki 67 抗原表达率高于良性肿瘤，提示Ki 67 可用于区分恶性和良性肿瘤。角蛋白的免疫组织化学分布显示，眼睑基底细胞癌由均一的肿瘤细胞组成，均表达相同的角蛋白，而鳞状细胞癌和皮脂腺癌则由多种分化的肿瘤细胞组成。