Mechanism of controlling blood-cell passage between the sinus endothelial cells

控制窦内皮细胞之间血细胞通道的机制

• 批准号: 07807005
• 负责人: UEHARA Kiyoko
• 金额: $ 1.28万
• 依托单位: Fukuoka University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 1995
• 资助国家: 日本
• 起止时间: 1995 至 1996
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-07807005/
• 关键词: spleen; Sinus epithelial cell; Adherens junction; Tight junction; Stress fiber; Actin filaments; アクチンフイラメント

Junctions between the sinus endothelial cells of rat spleen were examined by electron microscopy, using both freeze-fracture and detergent-extraction techniques. Adherens and tight junctions were observed. Adherens junctions were the predominant junctional structures between endothelial cells and were located on basolateral and lateral surfaces. At the basolateral adherens junctions, actin filaments were associated with the junctional membrane and were continuous with actin filaments in stress fibers. Cross-bridges were present in the interspaces of the adherens junctions, and spacings of the bridges were fairly regular. A form of tight junction, macula occludens, was also observed between the endothelial cells, it was not observed at every cellular apposition. Electron-dense material, adjoiningthe cytoplasmic surfaces of membranes in the tight junctions, separated the junctional membranes from masses of thin filaments. At basolateral tight junctions, the actin filaments were continuous with those in the stress fibers. Based on these observations, these two intercellular junctions were considered to play important roles in sinus functions.

使用冻结和清洁剂 - 萃取技术，通过电子显微镜检查了大鼠脾内皮细胞之间的鼻窦内皮细胞之间的连接。观察到粘附和紧密连接。粘附连接是内皮细胞之间主要的连接结构，位于基底外侧和侧面。在基底外侧的粘附连接处，肌动蛋白丝与连接膜相关，并且与胁迫纤维中的肌动蛋白丝连续相关。跨桥存在于粘附连接的空间中，桥梁的间距相当规律。在内皮细胞之间也观察到了一种紧密连接的大黄斑闭合剂，在每个细胞伴奏中都没有观察到。电子致密的材料，与紧密连接处的膜的细胞质表面相邻，将膜膜与细丝质量分开。在基底外侧紧密连接处，肌动蛋白丝与应力纤维中的丝是连续的。基于这些观察结果，这两个细胞间连接被认为在窦功能中起重要作用。

项目成果

K.Uehara & M.Miyoshi: "Junctions between the sinus endothelial cells of rat spleen" Cell and Tissue Research. 287,1. 187-192 (1997)

上原健