Quantitative analysis of nm23 gene product in human cancers

人类癌症中 nm23 基因产物的定量分析

基本信息

批准号：
07670220
负责人：
SATO Yuichi
金额：
$ 1.47万
依托单位：
KITASATO UNIVERSITY
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
1995
资助国家：
日本
起止时间：
1995 至 1996
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-07670220/
关键词：
nm23 gene product H1, H2 isoform immunoblotting immunohistochemistry densitometry nomal human tissues lung cancer tissues endometrial cancer tissues nm23 転移抑制遺伝子肺癌子宮体部癌

项目摘要

The total amount of nm23 protein, relative rations of H1 and H2 isoform (H2/H1) in human normal tissues, lung cancer tissues, endometrial cancer tissues were studied in combination with semiquantitative immunoblot technique followed densitometry and immunohistochemistry using monoclonal antibody against nm23 protein (Pan-242). All tissues including cancer tissues contained both isoform recognized in 20.5 kD as H1 and 18kD as H2 isoforms. In normal tissues, the level of total nm23 protein was higher in liver, kidney and adrenal gland, and lesser in heart and muscle tissues. H2 levels were higher than H1 levels in all tissues, examined, and their H2/H1 rations were varied from tissue to tissue. The immunohistochemistry revealed that the parenchymal cells were homogeneously stained in liver, pancreas and adrenal gland tissues and heterogeneously stained in gastric mucosa and kidney tissues. On the other hand, in lung cancers, both H1 and H2 levels in cancer tissues were higher than non-neoplastic lung tissues, and tumor H2/H1 ratio were lower than non-neoplastic ones in adenocarcinoma and squamous cell carcinoma of the lung. Our study indicate that nm23 protein levels in adenocarcinoma of the lung were significantly correlated with histologic differentiation of tumors, whereas they were not correlated with metastasis potentials and tumor stage. Furthermore, in endometrial cancers, the expression of nm23-H1 and H2 was not correlated with stage, metastasis, tumor size, myometrial invasion, estrogen receptor or menopause. It follows from the findings presented that the high expression o nm23-H1 and -H2 is positively correlated with histological differentiation.

NM23蛋白的总量，H1和H2亚型（H2/H1）在人正常组织中，肺癌组织，子宫内膜癌组织的总量与使用单核抗体抗体抗体抗体抗体（PAN）抗体（PAM23）的蛋白质（）24242242相结合，研究了肺癌组织，子宫内膜癌组织。包括癌症组织在内的所有组织都包含在20.5 kD中识别为H1和18KD的同工型，为H2同工型。在正常组织中，肝脏，肾脏和肾上腺的总NM23蛋白水平较高，心脏和肌肉组织的水平较小。在所有组织中，H2水平高于H1水平，并且其H2/H1的分量因组织而异。免疫组织化学表明，实质细胞在肝脏，胰腺和肾上腺组织中均匀染色，并在胃粘膜和肾脏组织中染色。另一方面，在肺癌中，癌症组织中的H1和H2水平都高于非肿瘤性肺组织，肿瘤H2/H1比低于腺癌和肺部鳞状细胞癌中的非肿瘤性肺部比。我们的研究表明，肺腺癌中的NM23蛋白水平与肿瘤的组织学分化显着相关，而它们与转移电位和肿瘤期无关。此外，在子宫内膜癌中，NM23-H1和H2的表达与阶段，转移，肿瘤大小，肌层浸润，雌激素受体或更年期无关。得出的结果表明，高表达O NM23 -H1和-H2与组织学分化正相关。