Studies on Reproductive Physiology and Artificial Reproduction in the Cheetah (Acinonyx jubatus)

猎豹（Acinonyx jubatus）生殖生理与人工繁殖的研究

基本信息

批准号：
07660380
负责人：
KATO Seishiro
金额：
$ 1.34万
依托单位：
KOBE UNIVERSITY
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
1995
资助国家：
日本
起止时间：
1995 至 1997
项目状态：
已结题

项目摘要

The Cheetah (Acinonyx jubatus) is listed on Appendix I of the CITES and as "Vulnerable" by the IUCN.Breeding this species in captivity traditionally has been difficult. Our long-term goal is to investigate reproductive physiology and to develop artificially reproductive techniques in the cheetah. This study is conducted in cooperation with Himeji Central Park, Himeji Municipal Zoo, Gunma Safari World, Nanki Shirahama Adventure World and Hamamatsu Zoological Garden. Thirty eight cheetahs (nineteen males and nineteen females) were used in this study, and the following results were obtained. 1. Semen characteristics were markedly inferior, particularly with respect to the incidence of pleiomorphic spermatozoa. 2. The naphthol yellow S and erythrosin B staining gave good definition of the sperm acrosome. 4. TEST-yolk was more effective diluent for sperm cryopreservation than the lactose solution (PDV), conventionally used in the U.S.A.4. In non-pregnant females, concentrations of the plasma steroid hormones, especially progesterone, repeated a rhythmic pattern, which ranged from 9 to 12 weeks. 5. Vaginal cytology could be available for pregnancy diagnosis. 6. Laparoscopic observation revealed that PMSG-hCG treatment was efficacious against follicular development and ovulation induction. 7. For reasons of anatomical feature of the uterine cervix, nonsurgically artificial insemination technique was not adequate. 8. Although the maturation rate was low, immature (germinal vesicle stage) ovarian oocytes could be stimulate to mature to telophase I in vitro.

Cheetah（cacinonyx jubatus）在Cites的附录I上列出，并被IUCN列为“脆弱的”。传统上，在圈养中breed在圈养中占据了这种物种。我们的长期目标是研究生殖生理学，并在猎豹中开发人为的生殖技术。这项研究是与Himeji Central Park，Himeji Municipal动物园，Gunma Safari World，Nanki Shirahama Adventure World和Hamamatsu Zoological Garden合作进行的。在这项研究中使用了三十八个猎豹（十九位男性和十九个女性），并获得了以下结果。 1。精液特征显着较低，特别是在多形精子的发生率方面。 2。萘酚黄色S和红细胞素B染色给出了精子属性体的良好定义。 4.测试叶果比乳糖溶液（PDV）更有效，该溶液（PDV）通常在美国使用。在非怀孕的女性中，血浆类固醇激素的浓度，尤其是孕酮，重复了一种节奏模式，范围为9至12周。 5。阴道细胞学可用于妊娠诊断。 6。腹腔镜观察表明，PMSG-HCG治疗有效地抵抗卵泡发育和排卵诱导。 7。出于子宫宫颈的解剖学特征的原因，非外部人工授精技术不足。 8。尽管成熟速率低，但可能会刺激卵巢卵母细胞（生发囊泡阶段）在体外刺激到末期I。