Development of a new method for the analysis of individual biological history of the aquatic creatures using synchrotron radiation X-ray fluorescence imaging

开发利用同步辐射X射线荧光成像分析水生生物个体生物学史的新方法

基本信息

批准号：
07556046
负责人：
TSUKAMOTO Katsumi
金额：
$ 8.38万
依托单位：
The University of Tokyo
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (A)
财政年份：
1995
资助国家：
日本
起止时间：
1995 至 1997
项目状态：
已结题

项目摘要

After the investigation for three years, the following results have been obtained.1.Sample Preparation : Methods of sample preparation for synchrotron radiation X-ray fluorescence microprobe analysis was developed. For the fish otolith preparation, sagaital plane with otolith core of 30mum thick was revealed to be suitable for the analysis.2.Investigation of finger-print elements : ICP-AES analysis of otoliths of various fishes of different origin disclosed that they contain Sr, Zn, Fe, Mg, Mn and Al as trace components, among which Sr is found to be most suitable for the indicator of the salinity change of their environmental water.3.Application of Synchrotron Radiation : Two-dimensional distributions of Ca, Sr and Fe were clarified by synchrotron radiation X-ray fluorescence microprobe. Measurements were made at Photon Factory, High Energy Accelerator Research Organization. Samples were excited by an X-ray of 17 keV generated by a Si (111) double-crystal monochromator. Two dimensional analysis of trace elemenls such as iron (average : around 100 ppm) was successfully carried out by using X-ray microbeam under the following condition : beam size, ca 100x1OO um2 and counting time of 10sec/step. Thus we have established the XRF imaging technique as a new important tool for characterization of the trace elements in the otoliths.4.Instrumentation : We have developed a new X-ray fluorescence imaging system for routine analysis of the trace elements in the hard tissues by using conventional laboratory X-ray source. This system utilizes monochromatic Mo Ka X-ray obtained by a curved Graphite monochoromator, which enables us to carry out high sensitivity analysis.5.Validation of the methods : To verify the method developed here, otoliths of the freshwater eel, masu salmon and lantern fish were analyzed. As the results, the individual migratory history was well demonstrated and thus, we conclude this method was sufficiently verified.

经过三年的研究，取得了以下成果： 1.样品制备：建立了同步辐射X射线荧光探针分析样品制备方法。对于鱼耳石制备，耳石核厚度为 30μm 的矢状平面适合分析。2.指纹元素研究：对不同来源的各种鱼类的耳石进行 ICP-AES 分析，发现它们含有 Sr、 Zn、Fe、Mg、Mn、Al为微量成分，其中Sr最适合作为其环境水体盐度变化的指示剂。3.同步加速器的应用辐射：通过同步辐射X射线荧光微探针阐明Ca、Sr和Fe的二维分布。测量是在高能加速器研究组织光子工厂进行的。样品被 Si (111) 双晶单色仪产生的 17 keV X 射线激发。使用X射线微束在以下条件下成功地对铁等痕量元素（平均：约100 ppm）进行了二维分析：光束尺寸，ca 100x100 um2，计数时间为10秒/步。因此，我们将XRF成像技术确立为表征耳石中微量元素的新的重要工具。4.仪器：我们开发了一种新的X射线荧光成像系统，用于对硬组织中的微量元素进行常规分析。使用常规实验室 X 射线源。该系统利用由弯曲石墨单色仪获得的单色莫卡X射线，这使我们能够进行高灵敏度分析。5.方法验证：为了验证这里开发的方法，淡水鳗鱼、马苏鲑鱼和灯笼的耳石对鱼进行了分析。结果，很好地证明了个体的迁徙历史，因此，我们得出结论，该方法得到了充分的验证。