Developement of rotating annular chromatographic reactor for continuous production of pectine oligosaccharide

连续生产果胶寡糖的旋转环形色谱反应器的研制

基本信息

批准号：
07555548
负责人：
YONEMOTO Toshikuni
金额：
$ 0.96万
依托单位：
Tohoku University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (B)
财政年份：
1995
资助国家：
日本
起止时间：
1995 至 1996
项目状态：
已结题

项目摘要

In order to develope a rotating annular chromatographic reactor for continuous production of pectin oligosaccharide, it is necessary to clarify the separation performance of the rotating annular chromatography and the kinetics of the consecutive depolymerization using the immobilized enzyme. The hydrolysis experiments of oligosaccharide using both suspended and immobilized enzymes were carried out under various conditions of the initial substrate and enzyme concentrations. Isomaltohexaose, the oligosaccharide of the polymerization degree of 6, was chosen as a substrate, because the mechanism of consecutive depolymerization was comparatively simple and the concentration could be easily analyzed. Endo-dextranase was used as a enzyme. A novel kinetic model for the suspended enzyme system was developed by introducing the selectivity of the enzyme for cleaving each alpha-1,6-glycosidic bond of the substrate. The selectivity coefficient was estimated at 0.411 and almost the same as the exper … More imental values. The model was extended for the immobilized enzyme system by taking into account the intraparticle mass transfer resistance. The model constants were estimated by fitting the extended model with the experimental data obtained under the various conditions.The kinetic constants for the immobilized enzyme system are much larger than those for the suspended enzyme system. The estimated values of effective diffusivities in the support particle were a few order of magnitude smaller than those in aqueous solution. A column reactor packed with the immoblized enzyme was designed and prepared. The experiments of the continuous hydrolysis were carried out using the column reactor under the various conditions of the flow rate, the substrate concentration and the enzyme concentration. A mathematical model for the continuous process was constructed on the basis of the kinetic model for the immobilized enzyme system. The simulated results by the model using the estimated values of the model constants in the immobilized enzyme system were in good agreement with the experimental results. Less

为了开发旋转的环形色谱反应器来连续产生果胶寡糖，有必要使用固定酶阐明旋转环形色谱法的分离性能和保守沉积的动力学。在初始底物和酶浓度的各种条件下，使用悬浮和固定酶进行寡糖的水解实验。选择6的聚合度的寡糖作为底物，因为连续沉积的机理相对简单，并且可以轻松分析浓度，因此选择了6的寡糖。内氧化物酶用作酶。通过引入酶清洁底物的每种α-1,6-糖苷键的选择性，开发了一种悬浮酶系统的新型动力学模型。选择性系数的估计为0.411，几乎与专家……更多的基值相同。通过考虑颗粒内传质电阻，该模型扩展了固定的酶系统。通过将扩展模型与在各种条件下获得的实验数据拟合来估算模型常数。固定酶系统的动力学常数比悬浮酶系统的动力学常数大得多。在支撑粒子中有效扩散的估计值比水溶液中的估计值小几个数量级。设计和制备了装有卸载酶的柱反应器。在流速，底物浓度和酶浓度的各种条件下，使用柱反应器进行连续水解的实验。根据固定酶系统的动力学模型构建了连续过程的数学模型。模型使用固定酶系统中模型常数的估计值进行的模拟结果与实验结果非常吻合。较少的