嗅覚受容器細胞におけるG蛋白質介在性情報変換の分子機構

G蛋白介导的嗅觉受体细胞信息转导的分子机制

• 批准号: 07458224
• 负责人: KURAHASHI Takashi
• 金额: $ 3.52万
• 依托单位: Osaka University (1997)Okazaki National Research Institutes (1995-1996)
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 1995
• 资助国家: 日本
• 起止时间: 1995 至 1997
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-07458224/
• 关键词: cAMP; olfactory cell; patch clamp; olfaction; suppression; adenylyl cyclase; phosphodiesterase; enzyme activity; フォスフォディエステラーゼ; イオンチャネル; eAMP; ファスホディエステラーゼ; 酵素活性測度

In the present study, we investigated the chemo-electrical transduction mechanism in the olfactory receptor cell, Following is the list of the specific subjects focused in the present study.(1) Mechanism of odorant adaptation : It was found that odorant adaptation in the olfactory receptor cell in regulated by a Ca feedback. The main target of the cytoplasmic Ca is found to be the transduction channel.(2) Contribution of T-type Ca channel on action potential generation : It has been generally believed that in olfactory receptor cell action potentials are triggered by Na channel. In the present study, membrane currents were investigated in detail. It was found that, in addition to Na channel, opening of T-type Ca channels play an important role for the generation of action potential.(3) Non-selective suppression by odorants of voltage-gated ionic channels(4) Modification of odorant perception by adrenaline : In this study adrenaline, a well-known soluble hormone, can modulate activity of olfactory receptor cell. This may explain how olfactory perception is influenced by emotional or physical states.

在本研究中，我们研究了嗅觉受体细胞中的化学电型转导机制，遵循本研究集中的特定受试者的列表。（1）气味适应的机制：发现嗅觉中的气味适应性在嗅觉中。受到CA反馈调节的受体细胞。发现细胞质Ca的主要靶标是转导通道。（2）T型Ca通道对动作电位产生的贡献：通常认为在嗅觉受体细胞动作电位中，Na通道触发了Na通道。在本研究中，详细研究了膜电流。已经发现，除了NA通道外，T型Ca通道的打开对于产生动作电位起着重要作用。（3）电压门控离子通道的气味抑制（4）修改odorant的气味抑制（4）肾上腺素的感知：在这项研究中，肾上腺素是一种众所周知的可溶性激素，可以调节嗅觉受体细胞的活性。这可以解释嗅觉如何受到情感或身体状态的影响。

项目成果

Kawai,Kurahashi & Kaneko: "Nor selective Suppression of Voltage-gated Cuvrents by Oderants in the Newt Olfactory Receptor cell" Journal of General Physiology. 109. 265-272 (1997)

仓桥河合

Kawai F,Kurahashi T & Kaneko A: "T-type Ca2+ Channel Lowers the Threshold of Spike Generation in the Newt" Olfactory Receptor Cell. 108. 525-535 (1996)

河合F、仓桥T

河合房夫・倉橋 隆: "嗅覚器" 生体の科学. 47. 482-485 (1996)

Fusao Kawai 和 Takashi Kurahashi：“嗅觉器官”生物体科学 47. 482-485 (1996)。

Kurahashi T & Menini A: "Mechanism of Odorant Adaptation in the Olfactory cell" Nature. 385. 725-729 (1997)

仓桥T

Kawai,Kurahashi & Kaneko: "Non selective Suppression of Voltage-gated carreuts by Odorants in the newt olfactory Receptor cells" Journal of General Physiology. 109. 265-272 (1997)

仓桥河合