ケージド化合物励起用UV照射・調光装置の開発的研究

笼状化合物激发紫外辐照及调光装置的进展研究

• 批准号: 07558112
• 负责人: KURAHASHI Takashi
• 金额: $ 6.91万
• 依托单位: Osaka University (1996-1997)Okazaki National Research Institutes (1995)
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (A)
• 财政年份: 1995
• 资助国家: 日本
• 起止时间: 1995 至 1997
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-07558112/
• 关键词: Caged compound; cAMP; ionic channel; Patch clamp; UV flash; Olfactory cell; Retina; computer program; 味細胞; コンピュータプログラム

Olfactory transduction is mediated by the adenylyl cyclase-cAMP system, and their adaptation is regulated by Ca-feedback within the cAMP metabolism. In a qualitative manner, outline of olfactory transduction system is now fairly well understood. However, in the view of quantification, there are still many unresolved questions, since in the field of olfaction research, it is so difficult to control the stimulus delivery In the present study, for response quantification, we aimed to quantify the stimulation to the olfactory receptor cell. To do so, we used caged cAMP and developed a new UV-flash source which can control UV intensity freely and rapidly. Using the whole-cell patch clamp method, we monitored membrane currents sensitive to cytoplasmic cAMP.Photolysis of cytoplasmic caged-cAMP caused rapid activation of cAMP-gated channels. Using this new system, we are planning to quantify the response shape and Ca-dependence of the adaptation in the olfactory receptor cell. In future, the same protocol will be used for the understanding of wide variety of cell functions.

嗅觉转导由腺苷酸环化酶-cAMP 系统介导，其适应由 cAMP 代谢内的 Ca 反馈调节。从定性的角度来看，嗅觉转导系统的轮廓现在已经被很好地理解了。然而，从量化的角度来看，仍然存在许多未解决的问题，因为在嗅觉研究领域，控制刺激传递非常困难。在本研究中，对于反应量化，我们的目标是量化对嗅觉的刺激受体细胞。为此，我们使用笼式 cAMP 并开发了一种新的紫外闪光源，可以自由、快速地控制紫外强度。使用全细胞膜片钳方法，我们监测了对细胞质cAMP敏感的膜电流。细胞质笼状cAMP的光解引起cAMP门控通道的快速激活。使用这个新系统，我们计划量化嗅觉受体细胞适应的响应形状和 Ca 依赖性。将来，相同的协议将用于理解各种细胞功能。

项目成果

倉橋 隆(共著): "「脳と神経」章 視覚と嗅覚の情報変換機構" 共立出版,

仓桥隆（合著者）：《‘大脑和神经’篇视觉和嗅觉的信息转换机制》Kyoritsu Shuppan，

Kurahashi T & Menini A: "Mechanism of Odorant adaptation in the afactry receptor cell" Nature. 385. 725-729 (1997)

仓桥T

Kurahashi T & Menini A: "Mechanism of Odorant Adaptation in the Olfactory Receptor Cell" Nature. 385. 725-729 (1997)

仓桥T

倉橋 隆(共著): "シリーズ・ニューバイオフィジックス6.生物のスーパーセンサー「匂いのセンサー」" 共立出版, 44-53 (1997)

Takashi Kurahashi（合著者）：“系列新生物物理学 6. 生物超级传感器‘气味传感器’”Kyoritsu Shuppan，44-53 (1997)