Structural analysis of digitalis-like substance for the promoter of essential hypertention

原发性高血压促进剂类洋地黄物质的结构分析

• 批准号: 07457618
• 负责人: ITOH Hajime
• 金额: $ 0.58万
• 依托单位: Kitasato University School of Allied Health Sciences
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 1995
• 资助国家: 日本
• 起止时间: 1995 至 1997
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-07457618/
• 关键词: essential hypertention; the third factor; digitalis-like substance; Na^+, K^+-ATPase

A sepecific inhibitor of sodium-potassium-activatedadenosine 5'-triphosphatase (Na^+/K^+-ATPase) in urines from the patients with hyperpiesia was purified and the biochemical properties of the inhibitor was characterized. It was found that the inhibitor was directly purified from human urine with various HPLC systems including the column for sugar separation. The purified inhibitor was eluted as a single peak by ion-exchage chromatography in the sugar analytical system. The inhibitor in the peak fraction was thought to be an inducer of essential hypertension, because the time-dependent decrease of the specific peak was exactly correlated with its inhibitory activity. The inhibitory activity of the inhibitor was reduced in a time-dependent manner. Under the conditions, there were no detectable a specific peak of the inhibitor, but four peaks were observd when the compounds were generated from the inhibitor by spontaneous alteration.This analytical system revealed the existence of sugars and amino groups in the inhibitor. Only a peak of these four compounds didn't passed through an amino-trap column. These results suggest that the Na^+/K^+-ATPase inhibitor has a sugar chain consisted of at least three sugars including amino groups, which may be responsible for the recognition of the specific receptor for Na^+/K^+-ATPase on the membrane of both smooth musle and adrenal basal cells.

纯化了高次尿症患者的尿液中尿液中钠磷酸钠激活腺苷（Na^+/k^+ - ATPase）的二种抑制剂，并表征了抑制剂的生化特性。发现抑制剂是从人类尿液中直接纯化的，其中包括各种HPLC系统，包括糖分离柱。纯化的抑制剂通过糖分析系统中的离子 - 售出色谱法洗脱为单个峰。峰分数中的抑制剂被认为是必需高血压的诱导剂，因为特定峰的时间依赖性降低与其抑制活性完全相关。抑制剂的抑制活性以时间依赖的方式降低。在条件下，没有可检测到的抑制剂的特定峰，但是当通过自发改变从抑制剂产生化合物时，观察到四个峰。这种分析系统揭示了抑制剂中糖和氨基的存在。这四种化合物的峰值没有通过氨基陷阱列。这些结果表明Na^+/k^+ - ATPase抑制剂的糖链由至少三个糖组成，包括氨基组，这可能是识别Na^+/K^+ATPase的特定受体的识别。在光滑的肌肉和肾上腺基底细胞的膜上。