Reversibility of Functional Transformation of Microbodies

微体功能转变的可逆性

基本信息

批准号：
07456053
负责人：
NISHIMURA Mikio
金额：
$ 4.93万
依托单位：
Okazaki National Rsearch Institutes
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (B)
财政年份：
1995
资助国家：
日本
起止时间：
1995 至 1996
项目状态：
已结题

项目摘要

1. In germinating fatty seedlings, microbodies are functionally transformed to leaf peroxisomes from glyoxysomes, and during greening to glyoxysomes from leaf peroxisomes during senesecence.Immunocytochemical studies have shown that glyoxysomes can exchange directly into leaf peroxisomes during greening and leaf peroxisomes are once again converted directly to glyoxysomes during senescence. To crarify the regulation underlying the microbody tranformation, transgenic Arabidopsis plants that expressed a fusion protein composed of the N-terminal region of and glyoxysomal citrate synthase (gCS) and beta-glucuromidase (GUS), were generated and their localization and processing were characterized by immunological and immunocytochemical methods. The fusion protein was transported into functionally different microbodies, such as glyoxysomes, leaf peroxisomes and unspecialized microbodies and was subsequently processed. These observations indicated that the transport of gCS is mediated by its a … More minoterminal presequence and that the transport system is functional in all plant microbodies. Therefore, it is unlikely that glyoxysomes and leaf peroxisomes possess different targeting machineries.2. Hydroxpyruvate reductase belongs to leaf-peroxisome specific enzymes and is accumulated in microbodies during greening. Two different hydroxypyruvate redutase (HPR1 and HPR2) are expressed in pumpkin. cDNA and genomic DNA cloning for HPR strongly suggests that HPR1 and HPR2 are produced by alternative splicing. Two different hydroxypryuvate reductase, HPR1 and HPR2, are shown to be localized in microbodies and in the cytosol respectively, suggesting the possibility that the microbody transition may be regulated by alternative splicing.3. Two proteins in glyoxysomal membranes with molecular masses of 31kDa and 28kDa were purified and characterized. The former was shown to be a novel ascorbate peroxidase. It was found that the amounts of these membrance proteins decreased during the microbody transition from glyoxysomes to leaf peroxisomes and that the large one was retained in leaf peroxisomes, whereas the small one could not be found in leaf peroxisomes after completion of the microbody transition. The results clearly showed that membrane proteins in glyoxysomes change dramatically during the microbody transition, as do the enzymes in the matrix. Less

1。在发芽的脂肪幼苗中，微型生物在表现为叶氧化物中的叶氧化酶体的叶片过氧化物中的叶片过氧化物中被扔掉的叶状体可以直接换成过氧化物体，并且在叶中再次将叶子过氧化物组合在叶中通过免疫学和免疫细胞化学方法，对乙二醇柠檬酸盐合酶（GC）和β-葡萄糖酶（GUS）（GUS）的N末端和处理。通过一个……在所有植物微型机构中都可以使用。 HPR1和HPR2在南瓜中表达。 .3。在微生物转变完成后，在叶氧气体中的膜蛋白结束后无法在叶片过氧化物中发现。