Mechanism of genetic regulation of rice to salt atress

水稻对盐胁迫的基因调控机制

• 批准号: 07456003
• 负责人: UCHIMIYA Hirofumi
• 金额: $ 4.93万
• 依托单位: Institute of Molecular and Cellular Biosciences, University of Tokyo
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 1995
• 资助国家: 日本
• 起止时间: 1995 至 1996
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-07456003/
• 关键词: rice; stress; salt; gene expression; mRMA; nucleotide; homeostasis; DNA; 塩ストレス; ストレス耐性; エネルギー代謝; 通気組織; プロテアソーム; タンパク質分解

Developmental and some biochemical responses of rice (Oryza sativa L.) seedlings to salt stress were studied. Shoot and more critically root growth were limited when the seedlings were exposed to moderate levels of salinity (NaCl). Anatomical observations revealed that under normal growth conditions, aerenchyma formation in rice roots occurred 3 days after seed germination. Development of aerenchyma was also accompanied by well developed sclerenchyma and vascular tissues in roots. In contrast, under salt stress aerenchyma formation was not observed in roots of comparable growth stages. Detailed histological observations showed the developmental retardation of sclerenchyma and vascular elements by salt stress. Salinity stress also caused several other anatomical changes of root tissues with regard to secondary wall formation in vascular tissues and some UV-fluorescent substances observed exclusively associated with sclerenchyma and vascular tissues.Comparison of protein profiles from salt-treated and untreated root tissues indicated that the synthesis of several polypeptides was significantly suppressed by salt stress. Appearance of these in normal tissues could be correlated with aerenchyma development. Data on further analysis using antibodies of several proteins and electron microscopy are presented.

研究了水稻（Oryza sativa L.）对盐胁迫的发育和某些生化反应。当幼苗暴露于中等水平的盐度（NaCl）时，芽和更重要的根生长受到限制。解剖学观察结果表明，在正常生长条件下，水稻根部发生在种子发芽后3天。气氛的发育还伴随着发育良好的硬核和根部的血管组织。相比之下，在可比生长阶段的根部未观察到在盐应激下形成的气氛。详细的组织学观察结果表明，盐胁迫通过盐胁迫和血管元素的发育迟缓。盐度胁迫还引起了根组织的其他几种解剖学变化，在血管组织中的次要壁形成方面，某些与硬核和血管组织仅相关的紫外线荧光物质。这些在正常组织中的外观可能与空气教育的发育相关。使用了几种蛋白质和电子显微镜的抗体进行进一步分析的数据。

项目成果

Yano,A.,M.Umeda and H.Uchimiya: "Expression of functional proteins of cDNA encoding rice nucleoside diphosphate kinase (NDK) in Escherichia coli and organ-related alteration of NDK activities during rice seed germination (Oryza sativa L.)" Plant Molecular

Yano,A.、M.Umeda 和 H.Uchimiya：“编码水稻核苷二磷酸激酶 (NDK) 的 cDNA 的功能蛋白在大肠杆菌中的表达以及水稻种子萌发过程中 NDK 活性的器官相关改变 (Oryza sativa L.)”

Samarajeewa,P.K., et al.: "Sodium Chloride Stimulates Adenylate Kinase Level in seedlings of salt-sensitive rice varieties." J.Plant Physiol.147. 277-280 (1995)

Samarajeewa,P.K. 等人：“氯化钠刺激盐敏感水稻品种幼苗中的腺苷酸激酶水平。”

Kawai, M.and H.Uchimiya: "Tissue specific localization of adenylate kinase in rice (Oryza sativa L.) plants" J.Plant Physiol.146. 239-242 (1995)

Kawai, M. 和 H.Uchimiya：“水稻 (Oryza sativa L.) 植物中腺苷酸激酶的组织特异性定位”J.Plant Physiol.146。

Kawai,M.: "Tissue specific localization of adenylate kinase in rice (Oryza sativa L.) plants." Journal of Plant Physiology. 146. 239-243 (1995)

Kawai,M.：“水稻 (Oryza sativa L.) 植物中腺苷酸激酶的组织特异性定位。”

Liu,J.: "Analysis of randomly isolated cDNAs from developing endosperm of rice (Oryza sativa L.) : Evaluation of expressed sequence tags,and expression levels of mRNAs." Plant Molecular Biology. 29. 685-689 (1995)

Liu, J.：“从水稻 (Oryza sativa L.) 发育中的胚乳中随机分离的 cDNA 的分析：表达序列标签和 mRNA 表达水平的评估。”