Structure, Behavior, and Gene Functions of Avian Sex Chromosomes

鸟类性染色体的结构、行为和基因功能

基本信息

批准号：
07044175
负责人：
MIZUNO Shigeki
金额：
$ 5.25万
依托单位：
Tohoku University
依托单位国家：
日本
项目类别：
Grant-in-Aid for international Scientific Research
财政年份：
1995
资助国家：
日本
起止时间：
1995 至 1997
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-07044175/
关键词：
chicken Oriental white stork Ratitae W chromosome Z chromosome lampbrush chromosome chromomere fluorescence in situ hybridization 性判別プローブ DNAメチル化性染色体ヘテロクロマチンゲノムライブラリー FISH マクロサテライト配列

项目摘要

In 1997, the last year of the 3 years long joint research, Professor H.Macgregor organized a symposium on Molecular Cytogenetics of Avian Chromosomes, which was held on the 1st and 2nd October at the University of Leicester, U.K., where about 20 scientists actively working in this field attended from Japan, U.K., Russia, France and Germany and presented up-to-date results of their research. S.Mizuno and co-investigators (A.Ogawa, T.Hori, M.Teranishi and Y.Itoh) presented following results : 1) chromosome walking on the chicken Z and W chromosomes by screening clones from the BAC library of the female chicken genomic DNA,starting with genomic clones located near the premeiotic pairing ends of each chromosome, search for genes by the exon-trapping procedure, and location of clones by fluorescence in situ hybridization (FISH) on the lampbrush Z-W bivalent from a chicken oocyte, 2) male hypermethylated (MHM) region found at the middle of the short arm of the chicken Z chromosome and female … More specific transcription of this region into heterogeneous, high molecular weight RANA localized in nuclei, 3) determination of the gender of Oriental white stork by PCR using primers from the sex chromosome-specific sequences which are related to the EE0.6 sequence on the chicken W chromosome, and 4) a common origin of Carinatae and Ratitae sex chromosome pairs as demonstrated by FISH with three genomic clones ; EE0.6 from W chromosome, ZOV3 and IREBP from Z chromosome of chickens.I.Solovei presented the results of joint experiments with A.Ogawa and S.Mizuno that Xhol- and EcoRI-family repetitive sequences on the chicken W chromosome forms individually specific chromomeres on the premeiotic lampbruch W chromosome and that chromomere sizes vary extensively in accordance with changes of their repetition frequencies. S.Mizuno and co-investigators visited Roslin Institute at Edinburgh before the Symposium to talk with David Burt on the chicken gene mapping project. They conducted joint experiments with I.Solovei at the laboratry of H.Macgregor, after the Symposium, and could demonstrate that the MHM region was located and transcribed in a particular pair of loops on the lampbrush W chromosome of chickens. Less

In 1997, the last year of the 3 years long joint research, Professor H. Macgregor organized a symposium on Molecular Cytogenetics of Avian Chromosomes, which was held on the 1st and 2nd October at the University of Leicester, U.K., where about 20 scientists actively working in this field attended from Japan, U.K., Russia, France and Germany and presented up-to-date results of their research. S.Mizuno and co-investigators (A.Ogawa, T.Hori, M.Teranishi and Y.Itoh) presented following results: 1) chromosome walking on the chicken Z and W chromosomes by screening clones from the BAC library of the female chicken genomic DNA,starting with genomic clones located near the premeiotic pairing ends of each chromosome, search for genes by the exon-trapping procedure, and location of通过荧光原位杂交（FIS）在鸡蛋细胞的lampbrush z-w二价上进行克隆，2）男性在鸡Z染色体和女性短臂的中间发现的男性高甲基（MHM）区域……在该区域更具体地转录该区域，以异质，高分子体重从核rana中定位于核rana，核能固定在核能上，3）是由核rana定位于核能，3）是由核能定位的，3）染色体特异性序列与鸡W染色体上的EE0.6序列相关，而4）与三个基因组克隆的鱼相证明，Carinatae和Carinatae和Ratitae性染色体对的共同起源； ee0.6来自鸡的Z染色体W染色体，ZOV3和IREBP。根据其重复频率的变化。 S.Mizuno和共同研究人员在研讨会之前访问了爱丁堡的Roslin Institute，与David Burt谈论鸡基因地图项目。他们在研讨会之后与H.MacGregor实验室进行了与i.solovei进行联合实验，并可以证明MHM区域位于鸡的Lampbrush W染色体上的一对特定环中并转录。较少的