Control of final gonadal maturation in the hybrid sturgeon, bester.

控制杂交鲟的最终性腺成熟，效果更好。

基本信息

批准号：
05556032
负责人：
YAMAUCHI Kohei
金额：
$ 0.96万
依托单位：
HOKKAIDO UNIVERSITY
依托单位国家：
日本
项目类别：
Grant-in-Aid for Developmental Scientific Research (B)
财政年份：
1993
资助国家：
日本
起止时间：
1993 至 1994
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-05556032/
关键词：
sturgeon spermatogenesis oogenesis sex steroid reproductive cycle vitellogenin ベステル LHRH-a ラジオイムノアッセイ法

项目摘要

Testicular development in the adult male F1 sturgeon hybrid, the bester (Huso huso L.female x Acipenser ruthenus L.male) was examined monthly in relation to serum sex steroid levels. Spermatogenesis lasted for 1 year, with meiosis generally starting September and spermatogenesis in November, although there was considerable variation in testiculal developmental stages between fish sampled monthly. Testicular development continued, slowly, during the winter months until April. Fish did not exhibit spontaneous spermiation, and phagocytotic cavity of Sertoli cells became prominent from May onwards. Androgen levels increased during spermatogenesis and remained high throughout the pre-spermiation period. In the degeneration stage, 11-ketotestosterone concentrations declined to low levels, b while testosterone (T) levels remained high. The serum concentration of 17alpha, 20beta-dihydroxy-4-pregnen-3-one (DHP) was low throughout the reproductive cycle. Based on these resultss, it is suggested … More that the time appropriate for induction of final maturation would be from November-December to April when the testes are in the late stage of development.Changes in serum levels of estradiol-17beta (E_2)T,DHP and vitellogenin (VTG), in cultured adult female were examined in relation to ovarian development during a 1-year sampling period. Considerable variationsin oocyte development were found among fish. Oocytes at previtellogeenic ssatge generally started to develop conconitantly with the degeneration of the first batch ofoocyte. In vitellogenic individuals, ovaries were comprised of more advanced oocytes with diameter ranging from 0.6 to 2.6 mm and in the post-vitellogenic class, oocytes attained their largest size while the geminal vesicle was migrating towards the animal pole. Oocytes with a migrating nucleus were maintained during the winter period and massive degeneration started in April-May without germinal vesicle breakdown (GVBD) or ovulation occurring. Seasonal changes in E2, T and VTG levels were well correlated with the advancement of oogenesis. Their levels increased during vitellogenesis, whereas in the posstvitellogenic (migratory nucleus) stage the levels of E2 declined from 2-4 ng/ml to 1-2 ng/ml and VTG from 4-10 ng/ml to 0.2-0.5 ng/ml while T levels remained high. In contrast, serum levels of DHP were constantly low throughout the reproductive cycle. These results indicate that the time appropriate for induction of artificial reproduction would be from October-November to April-May when the oocytes are in the late stages of the development. Less

每月检查成年雄性 F1 鲟鱼杂交体中最好的睾丸发育（Huso huso L.female x Acipenser ruthenus L.male）与血清性类固醇水平的关系，精子发生持续 1 年，减数分裂通常从 9 月开始，精子发生在 10 月。 11 月，尽管每月取样的鱼的睾丸发育阶段存在相当大的差异，但在冬季，直到 4 月，鱼的睾丸发育仍在缓慢进行。从 5 月起，支持细胞的吞噬腔变得突出，雄激素水平在精子发生过程中增加，并在整个精子形成阶段保持较高水平，在变性阶段，11-酮睾酮浓度下降至较低水平，而睾酮 (T) 水平仍然较高。根据这些结果，整个生殖周期中 17α, 20β-二羟基-4-孕烯-3-酮 (DHP) 的血清浓度较低。建议诱导最终成熟的时间为 11 月至 12 月至次年 4 月，此时测试处于开发后期。血清雌二醇-17β (E_2)T、DHP 和卵黄蛋白原水平的变化（ VTG），在一年的采样期内对培养的成年雌性鱼的卵母细胞发育进行了检查，发现卵母细胞在卵黄发生前存在相当大的变化。 ssatge 通常随着第一批卵母细胞的退化而开始发育，在卵黄发生个体中，卵巢由直径范围为 0.6 至 2.6 毫米的更先进的卵母细胞组成，在卵黄发生后类别中，卵母细胞达到最大尺寸，而胚芽则达到最大。冬季期间，囊泡向动物极迁移，并保留了带有迁移核的卵母细胞，并在四月至五月开始大规模退化，没有生发。囊泡破裂 (GVBD) 或排卵发生的 E2、T 和 VTG 水平的季节性变化与卵子发生的进展密切相关，它们的水平在卵黄发生期间增加，而在卵黄发生后（迁移核）阶段，E2 的水平从 2- 下降。 4 ng/ml 至 1-2 ng/ml，VTG 从 4-10 ng/ml 至 0.2-0.5 ng/ml，同时 T 水平保持较高水平。相比之下，在整个生殖周期中，血清DHP水平持续较低。这些结果表明，适宜诱导人工生殖的时间是10月至11月至4月至5月，此时卵母细胞处于发育后期。