Standardization and problems to measure prostanoids in the biological fluid.

测量生物体液中前列腺素的标准化和问题。

• 批准号: 03557039
• 负责人: ABE Keishi
• 金额: $ 10.56万
• 依托单位: TOHOKU UNIVERSITY
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Developmental Scientific Research (B)
• 财政年份: 1991
• 资助国家: 日本
• 起止时间: 1991 至 1993
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-03557039/
• 关键词: Prostacyclin; Thromboxane A_2; 11-dehydro-thromboxane B_2; 2, 3-dinor-6-keto-prostaglandin F_1alpha; Hypertension; Toxemia of pregnancy; Inflammation; Measurement; トロンボキサン(TXA_2); プロスタサイクリン(PGI_2); 11-dehydro-TXB_2; 2,3-dinor-b-keto PGF_1α; 6,

The method to measure 11-dehydro-thromboxane A_2(TXA_2) in the biological fluid was established by the previous research project. In this following project we performed the clinical application of this method in various human diseases as well as animal models of diseases. Over productions of TXA_2 were suspected in patients with myocardial infarction, cerebral infarction, diabetes mellitus, toxemia of pregnancy and bronchial asthma who showed higher urinary excretions of 11-dehydro-TXB_2, Physiological and pathophysiological roles of TXA_2 were elucidated. Furthermore, various immunoaffinity column and HPLC separation method were developed for the clean up of eicosanoids from the biological fluids and the HPLC-laser system to detect the small amount of eicosanoids was also developed.On the other hand, EIA kit for 2, 3-dinor-6-keto-prostaglandin (PG)F_1alpha, the stable metabolite of prostacyclin (PGI_2) was developed and we shared in the work for the standardization and clinical applic … More ation of this method. The separation of 2, 3-dinor-6-keto-PGF_1alpha from biological fluid was needed by either immunoaffinity column using anti-6-keto-PGF_1alpha antibody or anti-2, 3-dinor-6-keto-PGF_1alpha antibody or by HPLC before the measurement by EIA.Urinary excretion of 2, 3-dinor-6-keto-PGF_1alpha in man was about 100-400 pg/mg creatinine measured either by EIA or by GC/SIM method. There were no sex related differences and urinary levels were affected mainly by the systemic production rate rather than the renal production rate. Thus the 2, 3-dinor-6-keto-PGF_1alpha seems suitable as the index of systemic production rate of PGI_2. This method was useful to measure the levels in the tissue culture media and in the biological fluid from inflammatory tissue. We also applied clinically to measure the urinary excretion of levels in patients with hypertension, diabetes mellitus and toxemia of pregnancy. The establishment of the measurement of major stable metabolites of TXA_2 and PGI_2, in turn, made it possible to clarify the production balance between TXA_2 and PGI_2 in various diseases. Surplus TXA_2 production than PGI_2 was found in patients with essential hypertension as well as diabetes mellitus.Thus, most important problems to measure TXA_2 and PGI_2 in the biological fluid was dissolved by these projects and these methods seems to be useful tools to clarify the pathphysiological roles of TXA_2 and PGI_2 in man. Less

先前的研究项目建立了测量生物流体中11-脱氢含量A_2（TXA_2）的方法。在以下项目中，我们在各种人类疾病以及动物模型中对这种方法进行了临床应用。怀疑心肌违规，脑违规，糖尿病，糖尿病，妊娠毒血症和支气管哮喘的患者怀疑TXA_2的过多生产，这些患者表现出较高的尿液排泄，对11-脱氢-TXB_2的尿液排泄，生理和病理生理学的尿液学，TXA__2的生理和病理学roles。此外，开发了各种免疫亲和力柱和HPLC分离方法，用于清理生物学流体的类eic烷和HPLC激光系统，以检测少量的eicosanoids。另一方面，EIA KIT，EIA KIT，2，3-Dinor-6-keto-6-Keto-Pglostabilite fg _1 perecliite f _1 sal por sal por the stat stat stat stat stat stat stat stat stat stat stat stat stat state （PGI_2）是开发的，我们在标准化和临床应用的工作中共享了更多此方法。使用抗6-酮-PGF_1ALPHA抗体或抗2、3-DINOR-6-keto-pgf_1alpha抗体或通过HPLC通过HPLC进行EIA，EIA.UIA.UIA的极端eia.2 and-Extreme，使用2，3-二酮-6-酮-6-酮-6-keto-pgf_1alpha与生物流体的分离。人类中的3-Dinor-6-Keto-PGF_1Alpha约为100-400 pg/mg肌酐通过EIA或通过GC/SIM方法测量。没有与性别有关的差异，泌尿水平主要受系统性生产率而不是肾脏生产率的影响。 2，3-Dinor-6-Keto-PGF_1Alpha似乎适合作为PGI_2系统生产率的索引。该方法可用于测量组织培养基的水平以及炎症组织中的生物液中的水平。我们还在临床上应用于高血压，糖尿病和妊娠毒血症患者的尿极端水平。反过来，建立TXA_2和PGI_2的主要稳定代谢物的测量，使得在各种疾病中阐明TXA_2和PGI_2之间的生产平衡成为可能。这些项目中的生物学液体溶解在生物学液体中，测量TXA_2和PGI_2的最重要的问题是在具有必不可少的高血压和糖尿病的患者中发现的剩余TXA_2。较少的

项目成果

H.Schweer, C.O.Meese, O.Furst et al.: Anal.Biochem.164. 156 (1987)

H.Schweer、C.O.Meese、O.Furst 等人：Anal.Biochem.164。

K.Omata, N.G.Abraham and M.L.Schwartzman.: "Differential formations of renal cytochrome P450 dependent arachidonic acid metabolism in spontaneously hypertensive rats." J.Hypertens.10. S113 (1992)

K.Omata、N.G.Abraham 和 M.L.Schwartzman.：“自发性高血压大鼠肾细胞色素 P450 依赖性花生四烯酸代谢的不同形成。”

K.Takeuchi, T.Abe, N.Takahashi and K.Abe.: "Molecular cloning and intrarenal localization of rat prostaglandin E2 receptor EP3 subtype." Biochem.Biophys.Res.Commun.194. 885-891 (1993)

K.Takeuchi、T.Abe、N.Takahashi 和 K.Abe.：“大鼠前列腺素 E2 受体 EP3 亚型的分子克隆和肾内定位。”

Masako Watanabe,Takanori Watanabe,Noriyhou Hirasawa,Suetsugu Mue,Shigeki Muramatsu,Yoko Matsushita,Hidekuni Takahagi,pierre Braquet collette Broquet,Lawrence Lcuine,KA〓: "Preparation of immunoaffinity mini-columns for the analysis of platlet activating fa

Masako Watanabe,Takanori Watanabe,Noriyhou Hirasawa,Suetsugu Mue,Shigeki Muramatsu,Yoko Matsushita,Hidekuni Takahagi,pierre Braquet collette Broquet,Lawrence Lcuine,KA〓：“用于分析血小板活化因子的免疫亲和微型柱的制备

T.Ohno, M.Katori, H.Hayashi, K.Nishiyama and K.Saigenji. (Eds.M.Tsuchiya, et al.): "A preventive mechanism of capsaisin in microcirculation against ethanol-induced gastric mucosal injury rat." Microcirculation annual. 9. 121-122 (1993)

T.Ohno、M.Katori、H.Hayashi、K.Nishiyama 和 K.Saigenji。