Experimental transmission of hantavirus infection in laboratory rats

实验室大鼠汉坦病毒感染的实验传播

• 批准号: 03680040
• 负责人: DOHMAE Kayoko
• 金额: $ 1.41万
• 依托单位: Osaka University Rrsearch Institute for Microbial Siseases, Vrant-in-Aid for Scientific Research (c)
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (C)
• 财政年份: 1991
• 资助国家: 日本
• 起止时间: 1991 至 1993
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-03680040/
• 关键词: HFRS virus; Seoul type B-1 strain; maternal immunity; natural transmission; antibody titer; T-cill deficient nude rat

1. In order to study the mechanism of prevalence of Hantavirus infection, protecting effect of antibodies transferred form mother to infant was assessed. antibodys to hantavirus Seolul type B-1 strain. maternally transferred to neonates prevented lethal infection. However, the antibodies were not necessarily active enough to protect against persistent virus infection in neonates, Establishment of persisstent infection depends upon the level of virus titers inoculated,Neonates completely protected form active virus infection by maternal immunity do not show immune response to a secondary challenge of the same virus.2. We have developed a new natural transmission system of hantavirus in laboratory rats between immunologically intact (rnu/+) and their congenitally T-cell deficient nude (rnu/rnu) littermates.Nude rats dicharge more of active infectious viruses to cause prevalence of infection and die when infected. Furthermore, immunologically normal serpositive rats never cause infection to cagemate nude rats. Thus, nude rats are highly susceptible to the hantavirus infection.These laboratory animals and experimental system are useful models to study the mechanism of hantavirus infection.

1.为了研究汉坦病毒感染的流行机制，评估了母婴抗体的保护作用。汉坦病毒 Seolul 型 B-1 株抗体。母体转移给新生儿可预防致命感染。然而，抗体的活性不一定足以防止新生儿持续性病毒感染，持续性感染的建立取决于接种的病毒滴度水平，完全受到母体免疫保护免受活性病毒感染的新生儿不会表现出对二次攻击的免疫反应相同的病毒。2。我们在免疫完整的实验鼠（rnu/+）和先天性 T 细胞缺陷的裸鼠（rnu/rnu）同窝实验鼠中开发了一种新的汉坦病毒自然传播系统。裸鼠排出更多的活性传染性病毒，导致感染流行，感染后死亡。此外，免疫学正常的血清阳性大鼠不会引起笼养裸鼠的感染。因此，裸鼠对汉坦病毒感染高度敏感。这些实验动物和实验系统是研究汉坦病毒感染机制的有用模型。

项目成果

Yoshiki Niwa,Tsutomu Kasugai,et al: "Anemia and Mast Cell Depletion in Mutant Rats Are Homozygous at“white Spotting(Ws)"Locus" Bood. 78. 1936-1941 (1991)

Yoshiki Niwa、Tsutomu Kasugai 等人：“突变大鼠的贫血和肥大细胞耗竭在“白色斑点 (Ws)”基因座”Bood 处是纯合的。78。1936-1941 (1991)