The study of implantation mechanism using in vitro implantation model and embryo transfer system.

利用体外植入模型和胚胎移植系统研究植入机制。

• 批准号: 03670766
• 负责人: FUKAYA Takao
• 金额: $ 1.34万
• 依托单位: Tohoku University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (C)
• 财政年份: 1991
• 资助国家: 日本
• 起止时间: 1991 至 1993
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-03670766/
• 关键词: Early Embryo Development; 2 Cell Block; Free Radical; Scavenger; Extra Cellular Matrix; 2 cell block; free radical; 着床; 共培養; 脱落膜細胞; ファイブロネクチン; テネイシン; 胚; 胚移植

In vitro fertilization and embryo transfer(IVF-ET) program has been widely used as a final resort for the treatment of infertile women. However, the study of early embryo development and implantation mechanism is essential to improve the success rate of IVF-ET program. The purpose of this study is to reveal the relation between free radical-scavenger system and early embryo development, the interaction between the endometrial cell and early embryo development and the localization of extra cellular matrix in the human decidua.1)The development of mouse early embryo using co-cultrure system. The mouse early embryo development was promoted when embryo was cultured with epithelial cells obtained from rabbit oviduct. Further, when cell culture inserts was placed between embryo and epithelial cells the embryuo development was almost similar as without cell culture inserts, hence, not only epithelial cells but also some factors from oviduct affect the embryo development. In IGF-1 supplement e … More xperiments, the features of developed embryos were found to be normal, therefor we speculate tht IGF-1 is one of the important factors for early embryo development.2)The establishment of implantation model and attachment of mice early embryo. To establish the implantation model we used pseudo pregnant mouse decidual tissue. When the material was obtained in the peri-implantation stage, the cahracteristic features of cultured decidual cells was confirmed as endometrial stromal cell both chemically and microscopically, and 60% of blastocyst attached to the cultured decidual cells.3)The mice early embryo and free radical-scavenger system. To determine whether supplementing the culture medium with billrubin which is a strong scavenger of free radicals can help to overcome the mouse 2-cell block. On supplementing the medium with bilirubin, almost 80% of the embryos progressed to the 4-cell stage. This result suggests that bilirubin can improve early embryo development.4)The localization of extra cellular matrix in human decidua. The extra cellular matrix is an important factor for cell to cell interaction. We examined the localization of tenasin and found distinct localization in the decidua of human miscarriage materials. This result indicates that tenasin is related to re-construction of the degenerated tissue. Less

体外受精胚胎移植（IVF-ET）项目作为治疗不孕女性的最后手段已被广泛应用，但早期胚胎发育和着床机制的研究对于提高IVF-ET项目的成功率至关重要。本研究的目的是揭示自由基清除系统与早期胚胎发育的关系、子宫内膜细胞与早期胚胎发育的相互作用以及细胞外基质在人蜕膜中的定位。1）小鼠早期胚胎发育胚胎使用共培养系统，当胚胎与从兔输卵管获得的上皮细胞一起培养时，小鼠早期胚胎发育得到促进。此外，当细胞培养插入物置于胚胎和上皮细胞之间时，胚胎发育几乎与没有细胞培养插入物相似。因此，不仅是上皮细胞，而且输卵管的一些因素也会影响胚胎的发育。在实验中，发现发育的胚胎的特征是正常的，因此我们推测。 IGF-1是早期胚胎发育的重要因素之一。2)小鼠早期胚胎着床模型的建立采用假孕小鼠围周蜕膜组织作为材料。 -着床阶段，培养的蜕膜细胞的化学和显微镜特征均被证实为子宫内膜基质细胞，并且60％的囊胚附着在培养的蜕膜上3)小鼠早期胚胎和自由基清除剂系统 确定在培养基中补充胆红素（一种强自由基清除剂）是否有助于克服小鼠2细胞阻滞。几乎80%的胚胎进展到4细胞阶段。这一结果表明胆红素可以改善早期胚胎发育。4)细胞外基质在人类蜕膜中的定位是一个重要的因素。我们检查了 Tenasin 的定位，发现在人类流产材料的蜕膜中存在明显的定位，这一结果表明 Tenasin 与退化组织的重建有关。

项目成果

渡辺 正: "ヒト脱落膜におけるテネイシンの極在" 産婦人科の世界. 45. 593-596 (1993)

Tadashi Watanabe：“人类蜕膜中腱蛋白的极端存在”《妇产科世界》45. 593-596 (1993)。