The ovarian function and its local segulation

卵巢功能及其局部调节

基本信息

批准号：
06454462
负责人：
FUKAYA Takao
金额：
$ 3.84万
依托单位：
Tohoku University
依托单位国家：
日本
项目类别：
Grant-in-Aid for General Scientific Research (B)
财政年份：
1994
资助国家：
日本
起止时间：
1994 至 1995
项目状态：
已结题

项目摘要

In this study, we focused on the local regulation of steroidogenesis, growth factors and cell proliferation to evaluate the human follicular development in normal cycling ovary and polycystic ovary (PCO) using the methods of molecular endocrinology and biology.We found the sporadical expression of transcription factor (Ad4BP) of steroidgenic enzymes in preantral follicles. The localizations of scc, 3betaHSD and 17alpha were observed in preantral follicles and the mRNA expressions of these enzymes were confirmed in the theca cell. Expression of aromatase was generally observed in only one follicle (antral or mature follicle) per case in mid proliferative to premenstrual phase. The localization of androgen and estrogen receptor was observed in the antral follicle granulosa cells, and estrogen receptor was detected only in aromatase positive follicles. These results suggested that Ad4BP initially controls the function of steroidogenic enzymes and steroidogenic enzymes gradually express fr … More om primary follicles to mature follicles and we speculate that the expression of aromatase and estrogen receptor have an important role for the selection of dominant follicle.As regard growth factors, the localization and expression of TGFalpha was confirmed in theca cells and EGFR in granulosa cells at antral stage. This finding suggested that TGFalpha locally synthesized from theca cell and act as a paracrine fashion through EGFR.Ki67, PCNA and AgNORs which are the maker of cell proliferation increased significantly from antral to dominant follicles. Moreover, in antral follicle of luteal phase, immunostaning manner were similar as in follicular phase. These results suggest that the antral folicle in luteal phase has a potency to develop as dominant follicle.The local environment in PCO including steroidogenic enzymes, steroid receptors and Ki67 were similar as antral follicle of normally cycling ovary. These results suggest that follicles in PCO have a capability to develop for dominant follicle. Also this evidence may be one of the major cause for ovarian hyperstimulation syndrome (OHSS) by human menopausal gonadotropin treatment. In clinical study, we found that the endoscopic laser vaporization for PCO results in reduced OHSS which supports our molecular study for PCO. Less

在本研究中，我们重点关注类固醇生成、生长因子和细胞增殖的局部调节，利用分子内分泌学和生物学的方法来评估正常周期卵巢和多囊卵巢（PCO）中人类卵泡的发育。我们发现转录的零星表达腔前卵泡中类固醇生成酶的因子（Ad4BP）在腔前观察到 scc、3betaHSD 和 17α 的定位。在增殖中期至经前期，每个病例仅在一个卵泡（窦状或成熟卵泡）中观察到芳香酶的表达，并且在卵泡膜细胞中观察到了这些酶的 mRNA 表达。窦卵泡颗粒细胞中，雌激素受体仅在芳香酶阳性卵泡中检测到。这些结果表明，Ad4BP 最初控制类固醇生成酶的功能。类固醇生成酶从初级卵泡到成熟卵泡逐渐表达，我们推测芳香酶和雌激素受体的表达对于优势卵泡的选择具有重要作用。在生长因子方面，TGFα在卵泡膜中的定位和表达得到证实。细胞和胃窦期颗粒细胞中的 EGFR。这一发现表明 TGFα 由卵泡膜细胞局部合成，并通过 EGFR.Ki67 发挥旁分泌作用。细胞增殖标志物PCNA和AgNORs从窦卵泡到优势卵泡显着增加，而且在黄体期的窦卵泡中，免疫染色方式与卵泡期相似。这些结果表明，黄体期的窦卵泡具有抑制细胞增殖的能力。发育为优势卵泡。PCO的局部环境包括类固醇生成酶、类固醇受体和Ki67，与正常周期的窦卵泡相似这些结果表明，PCO 中的卵泡具有发育为优势卵泡的能力，并且这一证据可能是人类绝经期促性腺激素治疗中卵巢过度刺激综合征（OHSS）的主要原因之一。 PCO 激光汽化可减少 OHSS，这支持了我们对 PCO Less 的分子研究。