Analysis of the T-cells for the specific adoptive immunotherapy.

分析用于特定过继免疫疗法的 T 细胞。

• 批准号: 03670613
• 负责人: MASAKI Tadahiko
• 金额: $ 1.34万
• 依托单位: University of Tokyo
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (C)
• 财政年份: 1991
• 资助国家: 日本
• 起止时间: 1991 至 1993
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-03670613/
• 关键词: Tumor-draining lymphnode; Cytotoxicity; CD-8; Adoptive immunotherapy; Anti-CD3 antibody; Interleukin-2; Interferon-gamma; tumor draining; 養子免疫; 細胞障害性試験; インターロイキン2; インターフェロンガンマ; サイトカイン; T細胞; CTL; 腫瘍免疫; IL-2; 抗CD-3抗体; ILー2; CDー3

Tumor-draining lymphnode (DLN) cells, obtained from C57BL/6 mice with fibrosarcoma (MC-1), were activated and expanded with anti-CD3 monoclonal antibody followed by the culture with recombinant interleukin-2 These cells were non-cytolytic in vitro. However, they showed the specific anti-tumor efficacy to the pulmonary micrometastases of MC-1. The supernatant, obtained by co-culturing the activated DLN cells with MC-1, exhibited the specific production of interferon-gamma(IFN-gamma). The therapeutic effect correlated with the specific IFN-gamma production better than the cytolytic activity.

肿瘤引流淋巴结 (DLN) 细胞取自患有纤维肉瘤 (MC-1) 的 C57BL/6 小鼠，用抗 CD3 单克隆抗体激活并扩增，然后用重组白细胞介素 2 培养。这些细胞在体外是非溶细胞性的。然而，它们对MC-1的肺微转移表现出特异性的抗肿瘤功效。将活化的DLN细胞与MC-1共培养获得的上清液显示出干扰素-γ(IFN-gamma)的特异性产生。治疗效果与特异性 IFN-γ 产生的相关性优于与细胞溶解活性的相关性。

项目成果

鮫島 伸一 外7名: "Tumor-draining lymphnocytoを用いた養子免疫療法の検討" BIOTHERAPY. 8. 424-425 (1994)

Shinichi Samejima 和其他 7 人：“使用肿瘤引流淋巴细胞的过继性免疫疗法的研究”BIOTHERAPY 8. 424-425 (1994)。

S.Sameshima et al: "IFN-gamma) production of the tumor-draining lymphnode cell" BIOTHERAPY. 9. 708 (1995)

S.Sameshima 等人：“肿瘤引流淋巴结细胞的 IFN-γ 产生”生物疗法。

HIROHISA YOSHIZAWA,KEISUKE SAKAI,ALFRED E.CHANG,SUYU SHU: "Actication by AntiーCD3 of TumorーDraining Lymph Node Cells for Specific Adoptive Immunotherapy" Cellular Immunology. 134. 473-479 (1991)

HIROHISA YOSHIZAWA、KEISUKE SAKAI、ALFRED E. CHANG、SUYU SHU：“肿瘤引流淋巴结细胞的抗 CD3 对特异性过继免疫治疗的作用”细胞免疫学 134. 473-479 (1991)。