Role of locus coeruleus in cerebral circulation and catecholamine metabolism

蓝斑在脑循环和儿茶酚胺代谢中的作用

基本信息

批准号：
03670423
负责人：
ARAKI Nobuo
金额：
$ 1.28万
依托单位：
Keio University
依托单位国家：
日本
项目类别：
Grant-in-Aid for General Scientific Research (C)
财政年份：
1991
资助国家：
日本
起止时间：
1991 至 1993
项目状态：
已结题

项目摘要

(1)Neuronal activities in locus ceruleus respond to cerebral homodynamic changes induced by hypercapnia.When CO2 inhalation was applied, the significant increase in blood flow of the cerebral cortical tissue ipsilateral to the locus ceruleus was observed. The frequency of action potentials showed a gradual increase following the start of CO2 inhalation and reached a significant level by 40 seconds with maximum of 147 (〕SY.+-.〔) 22 % of the control value at 50 seconds. The cessation of CO2 inhalation induced a gradual recovery in the frequency of the action potential to the control level.(2)Cerebral intraparenchymal vasoconstriction induced by locus ceruleus stimulation.Cerebral blood volume(CBV) on the stimulated side gradually decreased following the start of 20Hz stimulation and reached a significant level by 80 seconds with a maximum of -0.15 (〕SY.+-.〔) 0.05vol% of the control value(p<0.05) at 140 seconds. CBV on the contralateral side was also reduced significantly. 50 Hz stimulation also resulted in a reduction in CBV bilaterally.(3)Locus ceruleus alters intraparenchymal vasoconrtrictive activities following subarachnoid hemorrhage.The CBV deresased significantly during the stimulation of locus ceruleus ; the decrease from the control value(6.3 vol%) was 0.14 (〕SY.+-.〔) 0.04 vol% at 80 seconds(p<0.05), 0.15 (〕SY.+-.〔) 0.03 vol% at 120 seconds. After the cessation of the stimulation, CBV showed a gradual recovery.(4)Catecholamine analysis by microdialysis technique.Perfusate from the cerebral cortex using microdialysis was analyzed by using high pressure liquid chromatography. We succeed in detectig norepinephrine, dopamine, DOPAC, HVA, serotonin, and 5-HIAA in same sample.

(1)蓝斑神经活动对高碳酸血症引起的脑同动力变化有反应。吸入CO2时，蓝斑同侧大脑皮层组织血流量显着增加，动作电位频率呈逐渐变化。开始吸入CO2后增加，并在40秒时达到显着水平，最大为147（〕SY.+-.〔）22％ 50秒时停止吸入CO2引起动作电位频率逐渐恢复至对照水平。(2)蓝斑刺激引起脑实质内血管收缩。刺激侧脑血容量(CBV)逐渐恢复。 20Hz 刺激开始后下降，并在 80 秒时达到显着水平，最大值为 -0.15 (〕SY.+-.〔) 140 秒时对照值的 0.05vol%（p<0.05）也显着降低，50 Hz 刺激也导致双侧 CBV 降低。蓝斑改变蛛网膜下腔出血后实质内血管收缩活性。刺激蓝斑时CBV显着下降蓝藻；80 秒时相对于对照值（6.3 vol%）的降低为 0.14 (〕SY.+-.〔) 0.04 vol%(p<0.05)，80 秒时为 0.15 (〕SY.+-.〔) 0.03 vol%刺激停止后120秒，CBV逐渐恢复。(4)儿茶酚胺分析。微透析技术。通过高压液相色谱法对来自大脑皮层的灌流液进行分析，成功地检测到同一样品中的去甲肾上腺素、多巴胺、DOPAC、HVA、血清素和5-HIAA。