Role of locus coeruleus in cerebral circulation and catecholamine metabolism

蓝斑在脑循环和儿茶酚胺代谢中的作用

基本信息

批准号：
03670423
负责人：
ARAKI Nobuo
金额：
$ 1.28万
依托单位：
Keio University
依托单位国家：
日本
项目类别：
Grant-in-Aid for General Scientific Research (C)
财政年份：
1991
资助国家：
日本
起止时间：
1991 至 1993
项目状态：
已结题

项目摘要

(1)Neuronal activities in locus ceruleus respond to cerebral homodynamic changes induced by hypercapnia.When CO2 inhalation was applied, the significant increase in blood flow of the cerebral cortical tissue ipsilateral to the locus ceruleus was observed. The frequency of action potentials showed a gradual increase following the start of CO2 inhalation and reached a significant level by 40 seconds with maximum of 147 (〕SY.+-.〔) 22 % of the control value at 50 seconds. The cessation of CO2 inhalation induced a gradual recovery in the frequency of the action potential to the control level.(2)Cerebral intraparenchymal vasoconstriction induced by locus ceruleus stimulation.Cerebral blood volume(CBV) on the stimulated side gradually decreased following the start of 20Hz stimulation and reached a significant level by 80 seconds with a maximum of -0.15 (〕SY.+-.〔) 0.05vol% of the control value(p<0.05) at 140 seconds. CBV on the contralateral side was also reduced significantly. 50 Hz stimulation also resulted in a reduction in CBV bilaterally.(3)Locus ceruleus alters intraparenchymal vasoconrtrictive activities following subarachnoid hemorrhage.The CBV deresased significantly during the stimulation of locus ceruleus ; the decrease from the control value(6.3 vol%) was 0.14 (〕SY.+-.〔) 0.04 vol% at 80 seconds(p<0.05), 0.15 (〕SY.+-.〔) 0.03 vol% at 120 seconds. After the cessation of the stimulation, CBV showed a gradual recovery.(4)Catecholamine analysis by microdialysis technique.Perfusate from the cerebral cortex using microdialysis was analyzed by using high pressure liquid chromatography. We succeed in detectig norepinephrine, dopamine, DOPAC, HVA, serotonin, and 5-HIAA in same sample.

（1）Ceruleus基因座中的神经元活性应对高碳酸血症引起的脑同源性变化。当二氧化碳吸入时，观察到脑皮质组织的血液流动显着增加，观察到Ceruleus ceruleus的脑皮质组织的血流显着增加。动作电位的频率显示在二氧化碳吸入开始后的等级增加，并在50秒时最大的控制值达到40秒，最大为147（> SY+ - 。〔）22％。二氧化碳吸入的停止导致动作潜力的频率恢复到控制水平。（2）在20Hz刺激开始后，由刺激的侧面逐渐减少了刺激的侧面逐渐减少，并以80秒的速度达到-15（25）。 140秒时，控制值的0.05Vol％（p <0.05）。对比侧的CBV也显着降低了。 50 Hz刺激也导致双侧CBV的降低。（3）ceruleus座基因座在蛛网膜下腔出血后改变了核内的血管结合活性。在刺激Ceruleus座基因座期间，CBV显着衍生酶。在80秒（p <0.05），0.15（>sy。+ - 。〔）0.03 vol％时，对照值（6.3 vol％）的减少为0.14（SY。+ - 。〔）0.04 vol％在120秒时。 After the cessation of the stimulation, CBV showed a grade recovery.(4)Catecholamine analysis by microdialysis technique.Perfusate from the cerebral cortex using microdialysis was analyzed by using high pressure liquid chromatography.We succeeded in detectig norepinephrine, dopamine, DOPAC, HVA, serotonin, and 5-HIAA in same sample.