Active Transport and Enzyme Activities in Various Metabolic Disorder of Herbivorous voles

草食田鼠各种代谢紊乱中的主动转运和酶活性

基本信息

批准号：
03660314
负责人：
OKI Yoshio
金额：
$ 0.51万
依托单位：
Nippon Veterinary and Animal Science University
依托单位国家：
日本
项目类别：
Grant-in-Aid for General Scientific Research (C)
财政年份：
1991
资助国家：
日本
起止时间：
1991 至 1992
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-03660314/
关键词：
^<42>Ar-^<42>K generator active transport Na^+, K^+-ATPase glucokinase ATP-citrate lyase acetly CoA carboxylase diabetes voles Na^+-K^+ ATPase NaーK ATPase ヘキソキナ-ゼ ATPークエン酸リア-ゼアセチルCoAカルボキシラ-ゼ

项目摘要

1. Active Transport and Na^+, K^+-ATPase in Herbivorous Voles(a)Active transport : Radioisotope ^<42>K produced by ^<42>Ar-^<42>K generator was orally injected into the voles. After 30 to 60 minutes. the ^<42>K was absorbed mainly from the forestomach and duodenum. The potassium ion was taken into the liver, kidney, heart and adipose tissues, but the uptake activities for ^<42>K were highest in the liver.(b)Na^+,K^+-ATPase : Na^+, K^+-ATPase activity was investigated in plasma membrane rich fractions from erythrocytes, liver, kidney and skeletal muscle of herbivorous voles. The activity of the ATPase in kidney was higher than those of other tissues. However, the Na^+,K^+-ATPase activities in plasma membranes of kidney in severe diabetic voles were lower than those in normal cases.2. Hepatic Glycolytic Enzyme and Lipogenic Enzyme Activities in Voles and Mice(a)Hepatic glycolytic enzyme : Hepatic glycolytic key enzyme, glucokinase in voles were considerablly lower than that in mice. In the monosodium aspartate (MSA)-treated voles, diabetes was induced. In the slight diabetic cases. the glucokinase activities increased about twice, but in the severe diabetic cases. the enzyme activities decreased about half.(b)Hepatic lipogenic enzymes : Hepatic ATP citrate lyase an acetyl-CoA Carboxylase in voles were considerably lower than those in mice. In the MSA-treated voles and mice, the plasma insulin concentrations increased significantly. The MSA-treated mice showed remarkably obesity and increase of the lipogenic enzyme activities. In the MSA-treated voles, signs of obesity were not observed and hepatic ATP citrate lyase activity increased significantly, but acetyl-CoA carboxylase activity did not increase.

1。活跃的传输和Na^+，k^+ - 在草食性田鼠中的ATPase（a）主动传输：放射性同位素^<42> k^<42> ar-^<42> k发电机被口服注入田鼠。 30至60分钟后。 ^<42> K主要从Forestomach和DuodeNum吸收。将钾离子带入肝脏，肾脏，心脏和脂肪组织中，但是^<42> k的摄取活性在肝脏中最高。 ^+ - ATPase活性在质膜，肝脏，肾脏，肾脏和草食性田鼠的骨骼肌肉中进行了研究。 ATPase在肾脏中的活性高于其他组织的活性。然而，在严重的糖尿病田鼠中肾脏质膜中的Na^+，K^+ - ATPase活性低于正常情况下的Na^+ - aTPase活性。2。田鼠和小鼠中的肝糖酵解酶和脂源性酶活性（A）肝糖酵解酶：肝糖酵解蛋白酶，田鼠中的葡萄糖酶比小鼠的葡萄糖酶要低。在单粉天冬氨酸（MSA）处理的田鼠中，诱导糖尿病。在轻微的糖尿病病例中。葡萄糖激酶的活性大约增加了两次，但在严重的糖尿病病例中。酶活性降低了大约一半。（b）肝脂肪生成酶：肝ATP柠檬酸裂解酶，田鼠中的乙酰辅酶A羧化酶低于小鼠。在MSA处理的田鼠和小鼠中，血浆胰岛素浓度显着增加。 MSA处理的小鼠表现出明显的肥胖和脂肪生物活性的增加。在经MSA处理的田鼠中，未观察到肥胖症的迹象，肝ATP柠檬酸裂解酶活性显着增加，但乙酰基-COA羧化酶活性并未增加。