signal transduction of small GTP binding proteins

小 GTP 结合蛋白的信号转导

基本信息

批准号：
03404022
负责人：
TAKAI Yoshimi
金额：
$ 20.22万
依托单位：
Kode University
依托单位国家：
日本
项目类别：
Grant-in-Aid for General Scientific Research (A)
财政年份：
1991
资助国家：
日本
起止时间：
1991 至 1993
项目状态：
已结题

项目摘要

There is the small GTP- binding protein (G protein) superfamily which consists of over fiftu members. All members have monomeric proteins with Mrs between 20,000 and 30,000 which exhibit both GDP/GTO-binding and GTP ase activities. In the present research project, we studied the functions and modes of activation of small G proteins. Ras is the representative of this su perfamily and is well known to regulate cell proliferation, differentiation, and transformation, but its modes of activation and action remain to be clarified. We characterized three GDP/GTP exchange proteins for Ras including Smq GDS, CDC25Mm, and msos. We identified a target protein for Ras and named it REKS, which phosphorylated MAP kinase and thereby activated it in a Ras-dependent manner. Accumulating evidence suggests that Rho regulates certain functions through an actomyosin system. By use of C3 exoenzyme and Rho GDI, we showed that Rho regulated cell morphogy, cell motility, membrane ruffling, cy-tokinesis, and smooth muscle contraction through an actomyosin system. Red family is known to regulate intracellular vesicle transport, such as exocytosis, endocytosis, and transcytosis. Rab3A is a member of Rab family which is particularly implicated in neurotransmitter release from the synapse. We isolated the cDNA encoding a target protein for Rab3A, which selectively in-teracted with the GTP-bound form of Rab3A, and named it rabphilin-3A.

有一个小的GTP结合蛋白（G蛋白）超家族，由过度的fiftu成员组成。所有成员的单体蛋白质具有20,000至30,000之间的MRS蛋白质，这些蛋白均表现出GDP/GTO结合和GTP ASE活动。在本研究项目中，我们研究了小G蛋白激活的功能和模式。 RAS是该SU的代表性的，众所周知可以调节细胞增殖，分化和转化，但其激活和作用方式仍然待阐明。我们表征了三种用于RA的GDP/GTP交换蛋白，包括SMQ GDS，CDC25MM和MSO。我们确定了RAS的靶蛋白，并将其命名为REK，该蛋白将MAP激酶磷酸化，从而以Ras依赖性方式激活它。积累的证据表明，Rho通过肌动球蛋白系统调节某些功能。通过使用C3外酶和Rho GDI，我们表明RHO调节细胞形态，细胞运动，膜荷叶皱纹，cy骨和平滑肌收缩，并通过肌动蛋白系统进行了平滑肌肉收缩。已知红色家族会调节细胞内囊泡的转运，例如胞吞作用，内吞作用和跨胞菌病。 Rab3a是Rab家族的成员，它特别涉及突触中的神经递质释放。我们分离了编码Rab3a的靶蛋白的cDNA，该cDNA与rab3a的GTP结合形式有选择地分离，并将其命名为Rabphilin-3a。