New Development in glycobiology

糖生物学的新进展

• 批准号: 03304050
• 负责人: MURAMATSU Takashi
• 金额: $ 12.35万
• 依托单位: Nagoya University School of Medicine (1993)Kagoshima University (1991-1992)
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Co-operative Research (A)
• 财政年份: 1991
• 资助国家: 日本
• 起止时间: 1991 至 1993
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-03304050/
• 关键词: Glycoprotein; Glycolipid; Ganglioside; Cell adhesion; Glycosyltransferase; Lectin; Mucin; Carbohydrate deficience; 統鎖生物学; プロチオグリカン; 発生分化; 統転移酵素; マクロファ-ジ; エンドサイト-シス; 癌転移; ガラクト-ス転移酵素; エンドグリコシダ-ゼ

Transfection and forced expression of alpha-1, 3-fucosyltransferase resulted in increased cell-substratum adhesion. Glycosyltransferases regulating glycolipid biosyntesis in human blood cells were identified. Structure of glycans bearing Le^a antigenic epitope was elucidated in the human colon. Regional difference was found in sulfation paltern of mucin carbohydrates isolated from the human stomach. Protein structure was determined for beta-1, 6-N-acetylglucosamine transferase, which is involved in the synthesis of Gsl-5. Mutation leading to Lewis blood group-negative blood type was elucidated at the DNA sequence level. A new hybrid glycosaminoglycan was produced by utilizing transglycosylation activity of hyaluronidase. Tyrosine residue(try-5) of macrophage lection was required for internalization of the lectin. Conagglutinin, a C-type lectin, was cDNA cloned and the genome structure was elucidated. Protein-carbohydrate linkage region of chondroitin sulfate was synthesized by organic synthesis.

α-1, 3-岩藻糖基转移酶的转染和强制表达导致细胞-基质粘附力增加。鉴定了调节人血细胞中糖脂生物合成的糖基转移酶。在人结肠中阐明了带有Le 1a 抗原表位的聚糖的结构。从人胃中分离的粘蛋白碳水化合物的硫酸化模式发现了区域差异。确定了参与 Gsl-5 合成的 β-1, 6-N-乙酰葡糖胺转移酶的蛋白质结构。在 DNA 序列水平上阐明了导致 Lewis 血型阴性血型的突变。利用透明质酸酶的转糖基活性产生了一种新的杂合糖胺聚糖。巨噬细胞凝集素的酪氨酸残基（try-5）是凝集素内化所必需的。结合凝集素（一种 C 型凝集素）的 cDNA 被克隆并阐明了其基因组结构。通过有机合成合成了硫酸软骨素的蛋白质-碳水化合物连接区域。

项目成果

Nakao, T., Kon, S., Ando, S., Miyatake, T., Yuki, N., Li Y.-T., Furuya, S.and Hirabayashi, Y.: "Novel lacto-ganglio type gangliosides with GM2-epitope in bovine brain which are the possible antigens responsible for inducing the amyotrophic lateral scleros

Nakao, T.、Kon, S.、Ando, S.、Miyatake, T.、Yuki, N.、Li Y.-T.、Furuya, S. 和 Hirabayashi, Y.：“新型乳神经节型神经节苷脂，

Muramatsu,T.: "Carbohydrate signals in metastasis and prognosis of human carcinomas" Glycobiology. 3. 291-296 (1993)

Muramatsu,T.：“人类癌症转移和预后中的碳水化合物信号”糖生物学。

Inagaki,F.: "Structural analysis of oligosaccharide chain of glyco-lipids by two-dimensional NMR" Mag.Res.Chem.30. 125-133 (1992)

Inagaki,F.：“通过二维 NMR 对糖脂寡糖链进行结构分析”Mag.Res.Chem.30。

Nishihara,H.: "Human α-1,3fucosyltransferase(Fuc-T-VI)gene is located at only 13 kb 3' to the Lewis type fucosyltransferase(Fuc-T-III)gene on chromosome 19" Biochem.Biophys.Res.Commun.190. 43-46 (1993)

Nishihara, H.：“人类 α-1,3 岩藻糖基转移酶 (Fuc-T-VI) 基因位于 19 号染色体上 Lewis 型岩藻糖基转移酶 (Fuc-T-III) 基因仅 13 kb 3 处” Biochem.Biophys.Res .通讯.190。43-46 (1993)

Sugiura,N.,Sakurai,K.,Hori,Y.,Karasawa,K.,Suzuki,S.and Kimata,K.: "Preparation of lipid-derivatized glycosaminoglycans to probe a regulatory function of the carbohydrate moieties of proteoglycans in cell-matrix interaction" J.Biol.Chem.268. 15779-15787 (1

Sugiura,N.、Sakurai,K.、Hori,Y.、Karasawa,K.、Suzuki,S. 和 Kimata,K.：“制备脂质衍生糖胺聚糖以探测细胞中蛋白聚糖碳水化合物部分的调节功能