Functional analysis of genes controlling germ-cell migration in zebrafish

控制斑马鱼生殖细胞迁移的基因的功能分析

• 批准号: 5242224
• 负责人: Professor Dr. Erez Raz
• 金额: --
• 依托单位: Institut für Zellbiologie
• 依托单位国家: 德国
• 项目类别: Priority Programmes
• 财政年份: 2000
• 资助国家: 德国
• 起止时间: 1999-12-31 至 2007-12-31
• 项目状态: 已结题
• 来源: https://gepris.dfg.de/gepris/projekt/5242224?language=en
• 关键词: Functional; analysis; genes; controlling; germ

In many organisms the primordial germ cells have to migrate from the position where they are specified towards the developing gonad where they generate gametes. Extensive studies of the migration of primordial germ cells in Drosophila, mouse, chick and Xenopus have identified somatic tissues important for this process and demonstrated a role for specific molecules in directing the cells towards their target. In preliminary studies we have characterized the migration process of the PGCs in wild type zebrafish embryos. To obtain information on the origin of the positional cues provided to the germ cells by somatic tissues during their migration, we analyzed the migration pattern in mutant zebrafish embryos. These early studies point at importance of the lateral border of the mesoderm as an intermediate target for the PGCs and identify a putative attraction center located at a specific dorsal-ventral and anteriorposterior position in early embryos. This proposal is aimed at identifying the molecular cues directing the PGCs towards their intermediate target at the lateral border of the mesoderm as well as identifying signaling molecules attracting the PGCs towards specific clustering positions during early somitogenesis stages. Towards this end a cDNA library enriched for transcripts expressed in the target tissue and in the germ cells themselves will be constructed. This library will be screened for cDNAs that are expressed in a pattern suggesting a role in PGC migration. These clones will be molecularly characterized and will be tested functionally for a role in directing the migration of the PGCs.

在许多生物体中，原始生殖细胞必须从指定的位置迁移到产生配子的发育中的性腺。对果蝇，小鼠，雏鸡和爪蟾中原始生殖细胞迁移的迁移的广泛研究已经确定了对这一过程重要的体细胞组织，并证明了特定分子在将细胞指向其靶标中的作用。在初步研究中，我们表征了野生型斑马鱼胚胎中PGC的迁移过程。为了获取有关细胞在迁移过程中通过体细胞组织提供给生殖细胞的位置提示的起源的信息，我们分析了突变斑马鱼胚胎中的迁移模式。这些早期研究指出，中胚层的侧边界是PGC的中间靶标，并确定位于早期胚胎中特定的背腹侧和前部位置的推定吸引力中心。该建议旨在确定将PGC引导到中胚层的侧边界的中间靶标的分子提示，并识别在早期发生物质发生期间吸引PGC朝着特定聚类位置吸引PGC的信号分子。为此，将构建一个富含在目标组织和生殖细胞本身中表达的转录本的cDNA文库。该库将以表明在PGC迁移中作用的模式表达的cDNA进行筛选。这些克隆将是分子表征的，并将在功能上测试，以指导PGC的迁移。