Functional analysis of genes controlling germ-cell migration in zebrafish

控制斑马鱼生殖细胞迁移的基因的功能分析

• 批准号: 5242224
• 负责人: Professor Dr. Erez Raz
• 金额: --
• 依托单位: Institut für Zellbiologie
• 依托单位国家: 德国
• 项目类别: Priority Programmes
• 财政年份: 2000
• 资助国家: 德国
• 起止时间: 1999-12-31 至 2007-12-31
• 项目状态: 已结题
• 来源: https://gepris.dfg.de/gepris/projekt/5242224?language=en
• 关键词: Functional; analysis; genes; controlling; germ

In many organisms the primordial germ cells have to migrate from the position where they are specified towards the developing gonad where they generate gametes. Extensive studies of the migration of primordial germ cells in Drosophila, mouse, chick and Xenopus have identified somatic tissues important for this process and demonstrated a role for specific molecules in directing the cells towards their target. In preliminary studies we have characterized the migration process of the PGCs in wild type zebrafish embryos. To obtain information on the origin of the positional cues provided to the germ cells by somatic tissues during their migration, we analyzed the migration pattern in mutant zebrafish embryos. These early studies point at importance of the lateral border of the mesoderm as an intermediate target for the PGCs and identify a putative attraction center located at a specific dorsal-ventral and anteriorposterior position in early embryos. This proposal is aimed at identifying the molecular cues directing the PGCs towards their intermediate target at the lateral border of the mesoderm as well as identifying signaling molecules attracting the PGCs towards specific clustering positions during early somitogenesis stages. Towards this end a cDNA library enriched for transcripts expressed in the target tissue and in the germ cells themselves will be constructed. This library will be screened for cDNAs that are expressed in a pattern suggesting a role in PGC migration. These clones will be molecularly characterized and will be tested functionally for a role in directing the migration of the PGCs.

在许多生物体中，原始生殖细胞必须从它们指定的位置迁移到发育中的性腺，在那里它们产生配子。对果蝇、小鼠、小鸡和非洲爪蟾原始生殖细胞迁移的广泛研究已经确定了对该过程重要的体细胞组织，并证明了特定分子在引导细胞朝向其目标的过程中的作用。在初步研究中，我们描述了野生型斑马鱼胚胎中 PGC 的迁移过程。为了获得有关体细胞迁移过程中体细胞向生殖细胞提供的位置线索起源的信息，我们分析了突变斑马鱼胚胎的迁移模式。这些早期研究指出中胚层侧缘作为 PGC 中间靶点的重要性，并确定了位于早期胚胎特定背腹和前后位置的假定吸引中心。该提案旨在确定引导 PGC 朝向中胚层侧缘中间靶标的分子线索，以及确定在早期体细胞发生阶段将 PGC 吸引至特定聚集位置的信号分子。为此，将构建富含在靶组织和生殖细胞本身中表达的转录物的cDNA文库。将筛选该文库中的 cDNA，这些 cDNA 的表达模式表明在 PGC 迁移中发挥作用。这些克隆将进行分子表征，并对其在指导 PGC 迁移中的作用进行功能测试。