The role glycosaminoglycans in controlling in vivo chemokine gradient formation, receptor activation and directed migration

糖胺聚糖在控制体内趋化因子梯度形成、受体激活和定向迁移中的作用

• 批准号: 128941087
• 负责人: Professor Dr. Erez Raz
• 金额: --
• 依托单位: Institut für Zellbiologie
• 依托单位国家: 德国
• 项目类别: Research Grants
• 财政年份: 2009
• 资助国家: 德国
• 起止时间: 2008-12-31 至 2013-12-31
• 项目状态: 已结题
• 来源: https://gepris.dfg.de/gepris/projekt/128941087?language=en
• 关键词: role; glycosaminoglycans; controlling; vivo; chemokine

Primordial germ cell (PGC) migration in zebrafish is guided by the chemokine SDF-1a that activates its receptor CXCR4b. This developmental process serves as an accessible model for chemokine-guided cell migration in the context of the live embryo. Despite the relatively detailed knowledge concerning the translation of SDF- 1a mediated receptor activation into directed migration, the molecular mechanisms controlling the distribution of the chemoattractant in vivo and the significance of the interaction with glycosaminoglycans (GAGs) for receptor activation are largely unknown. The research proposed here is aimed at defining the role glycosaminoglycans play in controlling germ cell migration through their effect on the distribution of SDF-1a and on receptor activation by this chemokine. The functional significance of these findings will be examined by in vitro and in vivo experiments in which the effect of the interaction between SDF-1a and GAGs on directing cell migration will be tested.

斑马鱼的原始生殖细胞 (PGC) 迁移受到趋化因子 SDF-1a 的引导，该趋化因子可激活其受体 CXCR4b。这一发育过程可作为活胚胎中趋化因子引导的细胞迁移的可用模型。尽管有关 SDF-1a 介导的受体激活转化为定向迁移的知识相对详细，但控制体内趋化剂分布的分子机制以及与糖胺聚糖 (GAG) 相互作用对受体激活的重要性尚不清楚。本文提出的研究旨在通过糖胺聚糖对 SDF-1a 分布和趋化因子受体激活的影响来确定糖胺聚糖在控制生殖细胞迁移中的作用。这些发现的功能意义将通过体外和体内实验进行检验，其中将测试 SDF-1a 和 GAG 之间的相互作用对指导细胞迁​​移的影响。