Characterization and induction of antitumor lymphokine derived from human lymph node cell
人淋巴结细胞来源的抗肿瘤淋巴因子的表征和诱导
基本信息
- 批准号:02670746
- 负责人:
- 金额:$ 1.54万
- 依托单位:
- 依托单位国家:日本
- 项目类别:Grant-in-Aid for General Scientific Research (C)
- 财政年份:1990
- 资助国家:日本
- 起止时间:1990 至 1992
- 项目状态:已结题
- 来源:
- 关键词:
项目摘要
This study was designed to investigate whether lymph node cells could exert an antiproliferative activity on tumor cells that are mediated through cytokines. The soluble antitumor activity of regional lymph node cells obtained from patients with cervical cancer was investigated by using a human tumor clonogenic assay (HTCA). A significant antiproliferative activity of the lymph node cells (LNCs) against a cervical cancer cell line, HeLa cells, was demonstrated by stimulation with either phytohemagglutinin (PHA) or concanavalin A(ConA), but not with interleukin-2(IL-2). In addition, a significant antiproliferative activity of the LNCs obtained from cervical cancer and endometrial cancer patients against a endometrial cancer cell line, RL95-2, was also demonstrated when they were stimulated by PHA. This antiproliferative activity was found mainly in nonadherent cells, possibly T-cells. LNCs produced both interferon gamma(IFNgamma) and tumor necrosis factor (TNF), and it was demonstrated that HeLa cells were sensitive to recombinant human IFNgamma and RL95-2 cells were sensitive to recombinant human TNF. These findings suggested the antitumor activities were attributed to IFNgamma and TNF, respectively. Moreover, about HeLa cells, it was confirmed that IFNgamma was responsible for the activity by using neutralizing experiments. These results indicate that human LNCs were able to exert an antiproliferative activity mediated through the cytokines by appropriate stimulation. In the two studies, LNCs could not demonstrate an antiproliferative activity by stimulation with IL-2, but peripheral blood lymphocytes, in some cases, exerted antiproliferative activity by stimulation with IL-2 on HTCA (submitted paper). In addition, LNCs produced cytokines, such as TNFalpha, TNFbeta, and IFNgamma when they were stimulated by IL-2 for longer duration (ongoing study). IL-2 stimulation on LNCs might be a promising procedure, so it is presently under intensive research investigation.
本研究旨在研究淋巴结细胞是否可以通过细胞因子介导对肿瘤细胞发挥抗增殖活性。使用人类肿瘤克隆形成试验(HTCA)研究了从宫颈癌患者获得的区域淋巴结细胞的可溶性抗肿瘤活性。通过植物血凝素 (PHA) 或刀豆球蛋白 A (ConA) 刺激,而非白细胞介素 2 (IL-2) 刺激,证明淋巴结细胞 (LNC) 对宫颈癌细胞系 HeLa 细胞具有显着的抗增殖活性。 。此外,从宫颈癌和子宫内膜癌患者获得的LNC在受到PHA刺激时也表现出了针对子宫内膜癌细胞系RL95-2的显着抗增殖活性。这种抗增殖活性主要存在于非贴壁细胞(可能是 T 细胞)中。 LNCs同时产生干扰素γ(IFNγ)和肿瘤坏死因子(TNF),并且证明HeLa细胞对重组人IFNγ敏感,RL95-2细胞对重组人TNF敏感。这些发现表明抗肿瘤活性分别归因于 IFNγ 和 TNF。此外,对于HeLa细胞,通过中和实验证实了IFNγ负责其活性。这些结果表明,人类 LNC 能够通过适当的刺激发挥细胞因子介导的抗增殖活性。在这两项研究中,LNC 无法通过 IL-2 刺激表现出抗增殖活性,但在某些情况下,外周血淋巴细胞通过 IL-2 刺激 HTCA 发挥抗增殖活性(已提交的论文)。此外,当 LNC 受到 IL-2 较长时间的刺激时,它们会产生细胞因子,例如 TNFα、TNFβ 和 IFNγ(正在进行的研究)。 IL-2 刺激 LNC 可能是一种有前途的方法,因此目前正在深入研究中。
项目成果
期刊论文数量(4)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
Toshiaki Saito: "Detection of Antitumor Lymphokine Production by Peripheral Blood or Lymph Node Lymphocytes Using Modified Clonogenic Assay" Cancer Immunol.Immunotherapy.
Toshiaki Saito:“使用改良的克隆形成试验检测外周血或淋巴结淋巴细胞产生的抗肿瘤淋巴因子”癌症免疫疗法。
- DOI:
- 发表时间:
- 期刊:
- 影响因子:0
- 作者:
- 通讯作者:
Masao Okadome: "Potential of Human Lymph Node Cells for Antitumor Activity Mediated by Interferon Gamma" Cancer. 68. 2378-2383 (1991)
Masao Okadome:“人类淋巴结细胞抗干扰素伽马介导的抗肿瘤活性的潜力”癌症。
- DOI:
- 发表时间:
- 期刊:
- 影响因子:0
- 作者:
- 通讯作者:
Okadome M,Saito T,Tsukamoto N,Sano M,Kamura T,Nakano H: "Potential of human lymph node cells for antitumor activity mediated by interferon gamma" Cancer. 68. 2378-2383 (1991)
Okadome M、Saito T、Tsukamoto N、Sano M、Kamura T、Nakano H:“人类淋巴结细胞抗干扰素γ介导的抗肿瘤活性的潜力”癌症。
- DOI:
- 发表时间:
- 期刊:
- 影响因子:0
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- 通讯作者:
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SAITO Toshiaki其他文献
SAITO Toshiaki的其他文献
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{{ truncateString('SAITO Toshiaki', 18)}}的其他基金
Elucidation of nitrous oxide production mechanisms focusing on the mRNA expression related to nitric oxide production and decomposition
阐明一氧化二氮产生机制,重点关注与一氧化氮产生和分解相关的 mRNA 表达
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$ 1.54万 - 项目类别:
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Characteristics of Nitrous Oxide Production Originated from Aerobic Denitrification of Nitrite by Poly-Phosphate Accumulating Organisms
聚磷酸盐积累生物亚硝酸盐好氧反硝化产一氧化二氮的特性
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12305068 - 财政年份:2000
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The progress of failure of mining developed cavern and its influence on the ground surface
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- 批准号:
10650915 - 财政年份:1998
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Interaction if Cell Adhesion Molecules and Tumor Cells on the Production of Matrix Metalloprotease in Gynecological Cancers
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06671662 - 财政年份:1994
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CELL ADHESION MOLECULES AND INVASION OF UTERINE CANCER
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05454452 - 财政年份:1993
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02650452 - 财政年份:1990
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63850137 - 财政年份:1988
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60550443 - 财政年份:1985
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$ 1.54万 - 项目类别:
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