CELL ADHESION MOLECULES AND INVASION OF UTERINE CANCER

细胞粘附分子与子宫癌的侵袭

• 批准号: 05454452
• 负责人: SAITO Toshiaki
• 金额: $ 2.43万
• 依托单位: Kyushu University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (B)
• 财政年份: 1993
• 资助国家: 日本
• 起止时间: 1993 至 1994
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-05454452/
• 关键词: Cell adhesion molecule; fibronectin; laminin; invasion; migration; uterine cancer; 接着因子; fibroblast

(1) An in vitro migration and invasion assay was used as the model system to study the effect of human fibronectin and laminin on the invasion of cervical carcinoma cells. Either purified fibronection or laminin enhanced the migration and invasion in a dose-dependent manner for all cervical carcinoma cell lines, HeLa, CAC-1 and TMCC.The pretreatment of cells with cell binding aminosequences, GRGDSP and/or YIGSR blocked the enhancement of cell invasion. Laminin enhanced the production of type IV collagenase by cell lines, HeLa and SiSo in a dose dependent manner. Effect of fibroblast derived from uterus was also evaluated. Fibroblasts in the lower chamber of the migration assay and invasion assay significantly enhanced the migration and invasion of cervical cancer cell lines. This effect was blocked by anti-fibronectin antibody. Furthermore, this effect was enhanced by the pretreatment of fibroblasts by vitamin-Cor TGF-b1.(2) Effect of lymphocytes on the growth and invasion of cervical cancer was investigated. Lymph node lymphocytes and peripheral blood lymphocytes exerted antiproferative effect against cervical cancer growth mediated through the production of interferon-gamma. The lymphocytes showed the promotion of invasion of cervical cancer.These results suggest the important role of cell adhesion molecules in the process of invasion of uterine cancer.

（1）将体外迁移和侵袭测定用作模型系统，以研究人纤连蛋白和层粘连蛋白对宫颈癌细胞侵袭的影响。纯化的纤维纤维纤维蛋白或层粘连蛋白都以剂量依赖性的方式增强了迁移和侵袭，用于所有宫颈癌细胞，HELA，CAC-1和TMCC。用细胞结合Aminosequences，GRGDSP和/或YIGSR的细胞预处理，使细胞的增强细胞的侵袭。层粘连蛋白以剂量依赖性的方式通过细胞系，HeLa和SISO增强了IV型胶原酶的产生。还评估了源自子宫的成纤维细胞的影响。迁移测定和入侵测定的下腔中的成纤维细胞显着增强了宫颈癌细胞系的迁移和侵袭。这种作用被抗原纤维蛋白抗体阻塞。此外，通过维生素-COR TGF-B1对成纤维细胞的预处理来增强这种作用。（2）研究了淋巴细胞对宫颈癌生长和侵袭的影响。淋巴结淋巴细胞和外周血淋巴细胞施加了针对宫颈癌生长的抗副作用作用，这是通过产生干扰素 - 伽马介导的。淋巴细胞表明促进宫颈癌的侵袭。这些结果表明细胞粘附分子在子宫癌侵袭过程中的重要作用。

项目成果

K.Sugihara,T.Saito,M.Okadome,et al.: "The promotion of invasion through the basement membrane of cervical carcinoma cells by fibronectin as a chemoattractant." Cancer Letter. 79. 167-173 (1994)

K.Sugihara、T.Saito、M.Okadome 等人：“纤连蛋白作为化学引诱剂促进宫颈癌细胞基底膜的侵袭。”

T.Kaku,N.Tsuruchi,N.Tsukamoto,et al.: "Reassessment of myometrial invasion in endometrial carcinoma." Obstetrics and Gynecology. 84. 979-982 (1994)

T.Kaku、N.Tsuruchi、N.Tsukamoto 等人：“子宫内膜癌肌层浸润的重新评估”。

Kenzo Sonoda, et al.: "Establishment of a new human uterine cervical adenocarcinoma cell line, SiSo, and its reactivity to anti-cancer reagents" International Journal of Oncology. 6. 1099-1104 (1995)

Kenzo Sonoda 等人：“新型人宫颈腺癌细胞系 SiSo 的建立及其对抗癌试剂的反应性”国际肿瘤学杂志。

Toshiaki Saito, et al.: "Antiproliferative lymphokine production by human peripheral blood lymphocytes and lymph node lymphocytes detected by a modified double layr soft agarose clonogenic assay" Lymphokine and Cytokine Research. 13. 55-62 (1994)

Toshiaki Saito 等人：“通过改良的双层软琼脂糖克隆形成试验检测人外周血淋巴细胞和淋巴结淋巴细胞产生的抗增殖淋巴因子”淋巴因子和细胞因子研究。

T.Saito,M.Okadome,K.Sugihara,et al.: "Antiproliterative lymphokine production by human peripheral blood lymphocytes and lymph node lymphocytes detected by a modified double laver soft aqarose clonogenic assay" Lymphokine and Cytokine Research. 13. 55-62 (

T.Saito、M.Okadome、K.Sugihara 等人：“通过改良的双紫菜软水凝胶克隆形成试验检测人外周血淋巴细胞和淋巴结淋巴细胞产生的抗增殖淋巴因子”淋巴因子和细胞因子研究。