CELL ADHESION MOLECULES AND INVASION OF UTERINE CANCER

细胞粘附分子与子宫癌的侵袭

基本信息

批准号：
05454452
负责人：
SAITO Toshiaki
金额：
$ 2.43万
依托单位：
Kyushu University
依托单位国家：
日本
项目类别：
Grant-in-Aid for General Scientific Research (B)
财政年份：
1993
资助国家：
日本
起止时间：
1993 至 1994
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-05454452/
关键词：
Cell adhesion molecule fibronectin laminin invasion migration uterine cancer 接着因子 fibroblast

项目摘要

(1) An in vitro migration and invasion assay was used as the model system to study the effect of human fibronectin and laminin on the invasion of cervical carcinoma cells. Either purified fibronection or laminin enhanced the migration and invasion in a dose-dependent manner for all cervical carcinoma cell lines, HeLa, CAC-1 and TMCC.The pretreatment of cells with cell binding aminosequences, GRGDSP and/or YIGSR blocked the enhancement of cell invasion. Laminin enhanced the production of type IV collagenase by cell lines, HeLa and SiSo in a dose dependent manner. Effect of fibroblast derived from uterus was also evaluated. Fibroblasts in the lower chamber of the migration assay and invasion assay significantly enhanced the migration and invasion of cervical cancer cell lines. This effect was blocked by anti-fibronectin antibody. Furthermore, this effect was enhanced by the pretreatment of fibroblasts by vitamin-Cor TGF-b1.(2) Effect of lymphocytes on the growth and invasion of cervical cancer was investigated. Lymph node lymphocytes and peripheral blood lymphocytes exerted antiproferative effect against cervical cancer growth mediated through the production of interferon-gamma. The lymphocytes showed the promotion of invasion of cervical cancer.These results suggest the important role of cell adhesion molecules in the process of invasion of uterine cancer.

(1)以体外迁移和侵袭实验为模型系统，研究人纤连蛋白和层粘连蛋白对宫颈癌细胞侵袭的影响。纯化的纤连蛋白或层粘连蛋白以剂量依赖性方式增强所有宫颈癌细胞系 HeLa、CAC-1 和 TMCC 的迁移和侵袭。用细胞结合氨基酸序列、GRGDSP 和/或 YIGSR 预处理细胞可阻断细胞的迁移和侵袭增强作用。入侵。层粘连蛋白以剂量依赖性方式增强细胞系 HeLa 和 SiSo 的 IV 型胶原酶的产生。还评估了来自子宫的成纤维细胞的作用。迁移实验和侵袭实验中下室的成纤维细胞显着增强了宫颈癌细胞系的迁移和侵袭。这种作用被抗纤连蛋白抗体阻断。此外，用维生素Cor TGF-b1预处理成纤维细胞可增强这种作用。(2)研究淋巴细胞对宫颈癌生长和侵袭的影响。淋巴结淋巴细胞和外周血淋巴细胞通过产生干扰素-γ对宫颈癌生长发挥抗增殖作用。淋巴细胞表现出对宫颈癌侵袭的促进作用。这些结果提示了细胞粘附分子在子宫癌侵袭过程中的重要作用。