Research on isolation and culture of forest tree species

森林树种分离培养研究

• 批准号: 02660154
• 负责人: IDE Yuji
• 金额: $ 1.41万
• 依托单位: The University of Tokyo
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (C)
• 财政年份: 1990
• 资助国家: 日本
• 起止时间: 1990 至 1992
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-02660154/
• 关键词: Protoplasts; Betula platyphylla; Quercus acutissima; Acacia mangium; Betula grossa; isolation; Colony formation; Callus formation; アカシア・マンギウム; ケヤキ; オノオレカンバ; コロニー; カルス再生; コロニ-; チョウセンヤマナラシ; マイクロカルス; サスペンションカルチャ-

In this research project, isolation and culture of protoplasts from forest tree species were investigated.Tree species belonging to Fagaceae and Betulaceae which are major broad leaved tree species in Japanese forests and Acacia mangium which is most promising tree species for tropical afforestration were used for research materials.As the results, protoplasts were successfully isolated from Quercus acutissima, Betula platyphylla var japonica, Betula grossa, Betula schmidtii, Zelkova serrata and Acacia mangium.And successful callus regeneration was induced by the culture of protoplasts isolated from somatic embryos of Quercus acutissima.By this research, basic components of enzymes solution and protocols for protoplast isolation from woody plants.

In this research project, isolation and culture of protoplasts from forest tree species were investigated.Tree species belonging to Fagaceae and Betulaceae which are major broad leaved tree species in Japanese forests and Acacia mangium which is most promising tree species for tropical afforestration were used for research materials.As the results, protoplasts were successfully isolated from Quercus acutissima, Betula platyphylla var japonica, Betula Grossa，Betula Schmidtii，Zelkova s​​errata和acacia杂种。成功的愈伤组织再生是由从Quercus acutissima的体细胞中分离出的原生质体培养的。

项目成果

Yuji IDE: "Isolation and culture of mesophyll protoplasts from in vitro subcultured Japanese white birch" Bull.Tokyo Univ. For.84. 53-58 (1991)

Yuji IDE：“体外传代培养的日本白桦叶肉原生质体的分离和培养”Bull.Tokyo Univ.

原口 雅人: "クヌギ不定胚からのプロトプラストの単離" 第102回日本林学会大会論文集. (1991)

Masato Haraguchi：“从锯齿体细胞胚胎中分离原生质体”日本林业学会第 102 届年会论文集（1991 年）。

Yuji Ide,: "Isolation and culture of protoplasts from Quercus acutissima shoot cultures." Bull. Tokyo Univ. For.85. 43-48 (1991)

Yuji Ide，：“麻栎芽培养物中原生质体的分离和培养。”

Yuji IDE: "Isolation and culture of protoplasts from Quercus acutissima shoot cultures" Bull.Tokyo Univ. For.85. 43-48 (1991)

Yuji IDE：“麻栎芽培养物中原生质体的分离和培养”Bull.Tokyo Univ.

原口 雅人: "クヌギ不定胚からのプロトプラストの単離" 102回日林論. 383-384 (1991)

原口正人：“从锯齿体细胞胚胎中分离原生质体”，第 102 期 Nihon Hayashiron，383-384（1991 年）。