Research on genetic variation and gene conservation of the tolerant poplar species for drought stress.

耐旱杨树种遗传变异及基因保护研究.

• 批准号: 05454077
• 负责人: IDE Yuji
• 金额: $ 4.22万
• 依托单位: The University of Tokyo
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (B)
• 财政年份: 1993
• 资助国家: 日本
• 起止时间: 1993 至 1995
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-05454077/
• 关键词: Arid tolerance; Populus spp.; Tissue culture; Gene conservation; 耐乾燥性

Methods for clonal propagation by tissue culture, callus culture and protoplast culture were established in this research project. The reactions of poplar clones for the stresses of low water potential, salinity and low pH conditions were also investigated.At first clonal propagation techniques of the poplars growing at the arid district in China was established. This made possible to conserve genetic resources of Chinese poplars which cannot grow in the field condition in Japan in vitro. This made the genetic conservation of temperate poplars possible too. The tolerance abilities for the stresses of low osmotic pressure and salinity and low pH of Populus euphratica, Populus tomentosa, Populus alba cv.pyramidalis X Populus tomentosa and Populus maximowiczii X Populus plantierensis were tested by using in vitro cultured shoots. Populus euphratica showed the highest tolerant for these three types of stress among test poplar clones. I think Populus euphratica has high genetic potential for the breeding of new cultivers for arid region. Finally protoplast culture was tested for these poplars. Successful colony formation was observed and the possibility of the genetic manipulation using protoplast culture was shown.

在该研究项目中确定了组织培养，愈伤组织培养和原生质体培养的克隆传播方法。还研究了杨树克隆对低水位，盐度和低pH状况的压力的反应。在中国干旱地区种植的杨树的首次克隆传播技术。这使得在日本体外无法在日本的田间状况下生长的中国杨树的遗传资源成为可能。这也使温带杨树的遗传保护也成为可能。低渗透压和盐度应力的耐受能力以及populus euphratica，populus tomentosa，populus alba cv.pyramidalis x populus tomentosa和populus maximowiczii x populus x populus X poppulus X poppul X poppul X poppullamidalis X Poppullamidalis X Poppullamidalis X Poppulensis的耐受能力。 Populus Euphratica在测试杨树中表现出对这三种压力的耐受性。我认为Populus euphratica对干旱地区的新培养基繁殖具有很高的遗传潜力。最后，对这些杨树测试了原生质体培养。观察到成功的菌落形成，并显示了使用原生质体培养的遗传操作的可能性。

项目成果

Yuji Ide: "In vitro Plantlet Regeneration from Petiole Culture of Poplar Hybrids and their competence for Adventitious Bud Formation" Bull.Tokyo Univ.For.91(印刷中). (1994)

Yuji Ide：“杨杂种叶柄培养的体外植株再生及其不定芽形成能力”Bull.Tokyo Univ.For.91（出版中）。

Kang,J.-M.: "Growth responce to the stress of low water potential,salinity and high pH in cultured shoot of Chinese popkars." J.For.Res.1(in print). (1996)

Kang，J.-M.：“中国杨树栽培芽的生长对低水势、盐度和高 pH 胁迫的反应。”

Ide,Y.: "In vitro plantlet regenration from petiole culture of Poplar hybrids and their competence for adventitious bud formation." Bull.Tokyo Univ.For.91. 127-135 (1994)

Ide，Y.：“杨杂种叶柄培养的体外植株再生及其不定芽形成的能力。”

Kang, J.-M.: "Growth response to the stress of low water potential, salinity and high pH in cultured shoot of Chinese poplars." J.For.Res.1 (in print). (1996)

Kang, J.-M.：“中国杨树培养芽对低水势、盐度和高 pH 胁迫的生长反应。”

Ide, Y.: "In vitro plantlet regeneration from petiole culture of Poplar hybrids and their competence for adventitious bud formation." Bull.Tokyo Univ.For.91. 127-135 (1994)

Ide，Y.：“杨杂种叶柄培养的体外植株再生及其不定芽形成的能力。”