A study on detoxication of dietary products of lipid peroxidation in digestive tract.

膳食制品对消化道脂质过氧化的解毒作用研究

• 批准号: 02660095
• 负责人: KANAZAWA Kazuki
• 金额: $ 1.6万
• 依托单位: Kobe University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (C)
• 财政年份: 1990
• 资助国家: 日本
• 起止时间: 1990 至 1992
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-02660095/
• 关键词: Lipid Peroxidation Products; Detoxify in Digestive Tract; Detection of Peroxidation Products; Nutrition; Linoleic Acid Hydroperoxide; Trilinolein Hydroperoxide; Toxicity of Lipoperoxide; Incorporation into Body; ペルオキシドの分解; 過酸化物のHPLC; 胃内解毒; 食餌脂質

Detoxifying ability of digestive tract for dietary products of lipid peroxidation was investigated in rats. The method for quantitative detection of peroxidation products from the animal stomach and intestines was estimated using a HPLC equipped with an amperometer and a UV detector. Linoleic acid hydroperoxides dosed orally easily disappeared in the stomach. A part of them was reduced to hydroxy linoleic acid or decomposed to aldehydes, and then transferred to jejunum and incorporated into body. The others were changed to keto-epoxy-octadecaenoic acid and remained in the gastric membrane. When trilinolein hydroperoxides were dosed orally, they disappeared in the stomach more slowly than linoleic acid hydroperoxides. Because trilinolein hydroperoxides were hydrolyzed to linoleic acid hydroperoxides in the stomach by lipase, it was considered that they were also decomposed to aldehydes, and then transferred to jejunum and incorporated into body. The disappearing rate of trilinolein hydroperoxides in the stomach was not affected by the nutritional conditions of rats on such a diet as high-lipid, high-protein, or high-carbohydrate. Thus, lipid hydroperoxides were decomposed in the stomach to aldehydes, which were incorporated into the body. It was therefore concluded that the toxicity of dietary products of lipid peroxidation must be due to their aldehydic products. I have already been made clear the hepatotoxicity by dietary aldehydes in the rats. By this project, a major components of the aldehydic products, 9-oxononanoic acid, was determined to be incorporated into liver. Then, another study on a role of 9-oxononanoic acid as one of bio-signals in the body is in progress.

研究了大鼠消化道对脂质过氧化膳食产物的解毒能力。采用配有安培计和紫外检测器的高效液相色谱仪对动物胃和肠中过氧化产物的定量检测方法进行了评估。口服亚油酸氢过氧化物很容易在胃中消失。其中一部分被还原为羟基亚油酸或分解为醛类，然后转移至空肠并融入体内。其他的则转变为酮环氧十八烯酸并保留在胃膜中。当口服三亚油酸氢过氧化物时，它们在胃中的消失速度比亚油酸氢过氧化物慢。由于三亚油酸氢过氧化物在胃中被脂肪酶水解为亚油酸氢过氧化物，因此认为它们也分解为醛，然后转移到空肠并进入体内。氢过氧化三亚油酸酯在胃中的消失速度不受高脂、高蛋白、高碳水化合物饮食等营养条件的影响。因此，脂质氢过氧化物在胃中分解成醛，并进入体内。因此得出结论，脂质过氧化膳食产品的毒性一定是由于其醛类产物造成的。我已经弄清楚膳食醛对大鼠的肝毒性。通过该项目，醛类产品的主要成分9-氧壬酸被确定会进入肝脏。然后，另一项关于9-氧代壬酸作为体内生物信号之一的作用的研究正在进行中。

项目成果

Kazuki KANAZAWA: "Effects and Bioactivity of lipid peroxidation products." Medical View. 4. (1993)

Kazuki KANAZAWA：“脂质过氧化产物的效果和生物活性。”

金沢 和樹: "Tissue injury induced by dietary products of lipid peroxidation" Free Radical and Antioxidants in Nutrition,Richelieu Frec Radical Series:Reheliae Press,London. (1993)

Kazuki Kanazawa：“脂质过氧化膳食产品引起的组织损伤”营养中的自由基和抗氧化剂，黎塞留 Frec Radical 系列：Reheliae Press，伦敦（1993 年）。

金沢 和樹: "Gastrical protection to lipoperoxides" Journal of Nutrition.

Kazuki Kanazawa：“对脂质过氧化物的胃保护”营养杂志。

Kazuki KANAZAWA: "Hepatotoxicity caused by dietary secondary products originating from lipid peroxidation." Nutritional and Toxicological Consequences of Food Processing(1991)ed. by M.Friedman, Plenum Press, New York. 237-253

Kazuki KANAZAWA：“源自脂质过氧化的膳食副产物引起的肝毒性。”

金沢 和樹: "Linoleic acid hydropercxides in digestive tract" Free Radical Biology a Medicine. 9. 108 (1990)

Kazuki Kanazawa：“消化道中的亚油酸氢过氧化物”自由基生物学和医学。 9. 108 (1990)