A study on detoxication of dietary products of lipid peroxidation in digestive tract.

膳食制品对消化道脂质过氧化的解毒作用研究

• 批准号: 02660095
• 负责人: KANAZAWA Kazuki
• 金额: $ 1.6万
• 依托单位: Kobe University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (C)
• 财政年份: 1990
• 资助国家: 日本
• 起止时间: 1990 至 1992
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-02660095/
• 关键词: Lipid Peroxidation Products; Detoxify in Digestive Tract; Detection of Peroxidation Products; Nutrition; Linoleic Acid Hydroperoxide; Trilinolein Hydroperoxide; Toxicity of Lipoperoxide; Incorporation into Body; ペルオキシドの分解; 過酸化物のHPLC; 胃内解毒; 食餌脂質

Detoxifying ability of digestive tract for dietary products of lipid peroxidation was investigated in rats. The method for quantitative detection of peroxidation products from the animal stomach and intestines was estimated using a HPLC equipped with an amperometer and a UV detector. Linoleic acid hydroperoxides dosed orally easily disappeared in the stomach. A part of them was reduced to hydroxy linoleic acid or decomposed to aldehydes, and then transferred to jejunum and incorporated into body. The others were changed to keto-epoxy-octadecaenoic acid and remained in the gastric membrane. When trilinolein hydroperoxides were dosed orally, they disappeared in the stomach more slowly than linoleic acid hydroperoxides. Because trilinolein hydroperoxides were hydrolyzed to linoleic acid hydroperoxides in the stomach by lipase, it was considered that they were also decomposed to aldehydes, and then transferred to jejunum and incorporated into body. The disappearing rate of trilinolein hydroperoxides in the stomach was not affected by the nutritional conditions of rats on such a diet as high-lipid, high-protein, or high-carbohydrate. Thus, lipid hydroperoxides were decomposed in the stomach to aldehydes, which were incorporated into the body. It was therefore concluded that the toxicity of dietary products of lipid peroxidation must be due to their aldehydic products. I have already been made clear the hepatotoxicity by dietary aldehydes in the rats. By this project, a major components of the aldehydic products, 9-oxononanoic acid, was determined to be incorporated into liver. Then, another study on a role of 9-oxononanoic acid as one of bio-signals in the body is in progress.

在大鼠中研究了消化道对脂质过氧化饮食产物的解毒能力。使用配备有安培计和紫外线检测器的HPLC估算了从动物胃和肠中定量检测过氧化产物的方法。口服剂量的亚油酸氢过氧化物在胃中很容易消失。其中一部分被还原为羟基亚油酸或分解为醛，然后转移到空肠并掺入体内。其他的变成了酮环氧 - 二十二烯酸，并留在胃膜中。当三琳氢过氧化物口服给药时，它们在胃中消失的速度比亚油酸氢过氧化物更慢。由于三琳盐水氢过氧化物通过脂肪酶在胃中的亚油酸氢过氧化物中水解，因此认为它们也被分解为醛，然后转移到空肠并掺入体内。胃中三琳盐水过氧化物的消失率不受大鼠在高脂质，高蛋白或高碳水化合物等饮食中的营养状况的影响。因此，将脂质氢过氧化物分解在胃中，并掺入体内。因此，得出的结论是，脂质过氧化的饮食产物的毒性必须是由于其醛产物引起的。我已经通过大鼠的饮食醛来清楚地清楚地表明了肝毒性。通过该项目，确定醛产物的主要成分，即9-氧酮酸酸，被确定为掺入肝脏中。然后，正在进行另一项关于9-氧支持者作为体内生物信号之一的作用的研究。

项目成果

金沢 和樹: "Tissue injury induced by dietary products of lipid peroxidation" Free Radical and Antioxidants in Nutrition,Richelieu Frec Radical Series:Reheliae Press,London. (1993)

Kazuki Kanazawa：“脂质过氧化膳食产品引起的组织损伤”营养中的自由基和抗氧化剂，黎塞留 Frec Radical 系列：Reheliae Press，伦敦（1993 年）。

Kazuki KANAZAWA: "Effects and Bioactivity of lipid peroxidation products." Medical View. 4. (1993)

Kazuki KANAZAWA：“脂质过氧化产物的效果和生物活性。”

金沢 和樹: "Gastrical protection to lipoperoxides" Journal of Nutrition.

Kazuki Kanazawa：“对脂质过氧化物的胃保护”营养杂志。

Kazuki KANAZAWA: "Hepatotoxicity caused by dietary secondary products originating from lipid peroxidation." Nutritional and Toxicological Consequences of Food Processing(1991)ed. by M.Friedman, Plenum Press, New York. 237-253

Kazuki KANAZAWA：“源自脂质过氧化的膳食副产物引起的肝毒性。”

金沢 和樹: "Zinoleic acid hydroperoxide in digestive tract" Free Radical Biology & Medicin. 9. 108 (1990)

Kazuki Kanazawa：“消化道中的亚油酸过氧化氢”自由基生物学与医学，9. 108 (1990)。