Research of Bioavailability of Dietary Polyphenols in the Cells

膳食多酚在细胞内生物利用度的研究

基本信息

批准号：
14360076
负责人：
KANAZAWA Kazuki
金额：
$ 9.6万
依托单位：
Kobe University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (B)
财政年份：
2002
资助国家：
日本
起止时间：
2002 至 2004
项目状态：
已结题

项目摘要

Bioavailability of dietary polyphenols was investigated using intestinal Caco-2, hepatic HepG2 and colonic WiDr cells.1.Apigenin-O-glucoside was incorporated into Caco-2 cells after hydrolyzed to aglycone, but its C-glucoside was not. The aglycone was released to the basolateral phase by 0.07% of the closed and its conjugated forms was by 1.1%.2.Various flavonoids were added to HepG2 cells and determined in the incorporated amounts into nuclei. They gave the maximum incorporation at 30 and 60 min after incubation. When the nuclei were exposed to active oxygens, flavonoids that possessed catechol structure in B ring were available to suppress formation of a DNA-oxidized products 8-OHdG.3.Flavonol type of flavonoids was specifically transformed to a novel metabolite, 3-amino forms, at the absorption step. The amino forms maintained the potencies for antioxidative activity and for protein fluction-modulating and additionally were refractory to be conjugated. Amino forms were considered to be an active form of flavonoids in the body.4.When a mixture of various polyphenols was added to Caco-2 cells, a prenyl phenol artepillin C was easily incorporated and released to the basolateral phase without receiving any conjugations.5.The artepillin C was also incorporated into HepG2 cells unchanged in form and suppressed the oxidation of intracellular DNA in a dose-dependent manner. Oral dose of artepillin C significantly prevent a formation of aberrant crypt foci in mouse colon. The dose of artepillin C to WiDr induced the cell arrest at G_0/G_1 phase through stimulating expression of p21.Thus, flavonols and prenyl phenol were found to be bioavailable since the former was metabolized to more active forms and the latter was refractory to be conjugated in the absorption prpcess.

利用肠道Caco-2、肝脏HepG2和结肠WiDr细胞研究了膳食多酚的生物利用度。1.芹菜素-O-葡萄糖苷水解为苷元后进入Caco-2细胞，但其C-葡萄糖苷则没有。糖苷配基以闭合时的0.07%释放到基底外侧相，其结合形式为1.1%。2.将各种黄酮类化合物添加到HepG2细胞中并测定进入细胞核的掺入量。他们在孵育后 30 和 60 分钟时达到最大掺入量。当细胞核暴露于活性氧时，B环中具有儿茶酚结构的黄酮类化合物可抑制DNA氧化产物8-OHdG的形成。3.黄酮醇型黄酮类化合物被特异性转化为新型代谢物3-氨基形式，在吸收步骤。氨基形式保持了抗氧化活性和蛋白质波动调节的效力，并且另外难以缀合。氨基形式被认为是体内黄酮类化合物的活性形式。4.当将各种多酚的混合物添加到Caco-2细胞中时，异戊二烯基苯酚artepillin C很容易掺入并释放到基底外侧相，而不接受任何结合。 5.artepillin C也以不变的形式掺入HepG2细胞中，并以剂量依赖的方式抑制细胞内DNA的氧化。口服阿替匹林 C 可以显着防止小鼠结肠中异常隐窝病灶的形成。 Artepillin C 到 WiDr 的剂量通过刺激 p21 的表达诱导细胞停滞在 G_0/G_1 期。因此，发现黄酮醇和异戊烯基苯酚具有生物利用度，因为前者被代谢为更活跃的形式，而后者难以缀合在吸收过程中。

项目成果

期刊论文数量（122）

专著数量（0）

科研奖励数量（0）

会议论文数量（0）

专利数量（0）

T.Hashimoto et al.: "Preventive effects of food components on caspase-8-mediated apoptosis induced by dietary carcinogen, Trp-P1, in rat mononuclear cells"ACS Symposium Series. 851. 128-140 (2003)

T.Hashimoto 等人：“食物成分对饮食致癌物 Trp-P1 诱导的大鼠单核细胞中 caspase-8 介导的细胞凋亡的预防作用”ACS 研讨会系列。

DOI：
发表时间：
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影响因子：
0
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Suppression of cytochrome P4501 a subfamily in mouse liver by oral intake of polysaccharaides from mushroom, Lentinus edodes and Agaricus blazei.

通过口服蘑菇、香菇和姬松茸中的多糖抑制小鼠肝脏中的细胞色素 P4501 亚家族。

DOI：
发表时间：
2003
期刊：
American Chemical Society, ACS Symposium Series 851
影响因子：
0
作者：
Hitoshi Ashida;Takashi Hashimoto;Yuji Nonaka;Itsuko Fukuda;Kazuki Kanazawa;Gen-ichi Danno;Ken-ichiro Minato;Sachiko Kawakami;Masashi Mizuno
通讯作者：
Masashi Mizuno

A tryptophan pyrolysis product, 3-amino-1,4-dimethyl-5H-pyrido [4,3-b]indole (Trp-P-1) but not its metabolite induces apoptosis in primary cultured rat heaptocytes.

色氨酸热解产物 3-氨基-1,4-二甲基-5H-吡啶并[4,3-b]吲哚 (Trp-P-1) 但其代谢物不会诱导原代培养的大鼠肝细胞凋亡。

DOI：
发表时间：
2003
期刊：
American Chemical Society, ACS Symposium Series 851
影响因子：
0
作者：
Bunsyo Shiotani;Hitoshi Ashida;Yuji Nonaka;Takashi Hashimoto;Kazuki Kanazawa;Gen-ichi Danno
通讯作者：
Gen-ichi Danno

Equol a metabolite of daizein, ihibits bone loss in ovariectomized mice.

雌马酚是大豆甙元的代谢物，可抑制卵巢切除小鼠的骨质流失。

DOI：
发表时间：
2004
期刊：
Journal of Nutrition 134
影响因子：
0
作者：
Maiko Fujioka;Mariko Uehara;Jian Wu;Herman Adlercreutz;Kazuharu Suzuki;Kazuki Kanazawa;Ken Takeda;Kazuhiko Yamada;Yoshiko Ishimi
通讯作者：
Yoshiko Ishimi

Suppressive effect of polysaccharides from the edible and medicinal mushrooms, Lentinus edodes and Agaricus blazei, on the expression of the cytochrome P450s in mice

食药用蘑菇香菇、姬松茸多糖对小鼠细胞色素P450s表达的抑制作用