A Rapid Analysis System of Protein Crystal Structures

蛋白质晶体结构快速分析系统

基本信息

项目摘要

1. After a personal IRIS graphics workstation was introduced into our laboratory, we checked its graphic and arithmetic performances at first. In result, we found that it has enough performance to realize the object of this project. Then we adapted the TOM program, a graphics fitting program for macromolecules originally coded by Cambillau (1987), to the Personal IRIS workstation. 2. Using a mainframe computer, we optimized thoroughly the stereochemically restrained least-squares refinement program PROLSQ (Hendrickson and Konnert, 1980) modified by us so that the execution time might become as short as possible. After the optimization we adapted the PROLSQ program to the Personal IRIS workstation, and improved the interface between the PROLSQ and TOM programs so that crystallographers might refine protein structures easily and quickly. 3. In order to see if this refinement system works well, we refined the crystal structure of the complex between microbial ribonuclease Ms and its inhibitor, 3'-guanylic acid, using the system. Its coarse atomic coordinates had been obtained by molecular replacement methods. The refinement system worked quite efficiently. It is the proof of the efficiency of the refinement system that for the past only one year we have finished refining seven kinds of protein structures, (1) RNase Ms*3'-GMP complex (2) Subtilisin-SSI complex (3) RNase Ms*2'-GMP complex (4) RNase Ms*GfpC complex (5) Trypsin-SSI complex (6) Interferon-beta and (7) RNase Rh, using this refinement system.
1。在将个人IRIS图形工作站引入我们的实验室之后,我们首先检查了其图形和算术性能。因此,我们发现它具有足够的性能来实现该项目的对象。然后,我们改编了Tom程序,这是一个最初由Cambillau(1987)编码的大分子的图形拟合程序,用于个人IRIS工作站。 2。使用大型计算机,我们对立体化学上最小二乘的精炼计划ProLSQ(Hendrickson and Konnert,1980)进行了彻底优化,以使执行时间尽可能短。优化后,我们将ProLSQ程序调整为个人IRIS工作站,并改进了ProLSQ和TOM程序之间的接口,以便晶体学者可以轻松,快速地完善蛋白质结构。 3。为了查看此改进系统是否效果很好,我们使用该系统完善了微生物核糖核酸酶MS及其抑制剂3'-瓜尼酸酯之间的晶体结构。它的粗原子坐标是通过分子替代方法获得的。改进系统的运作效率很高。 It is the proof of the efficiency of the refinement system that for the past only one year we have finished refining seven kinds of protein structures, (1) RNase Ms*3'-GMP complex (2) Subtilisin-SSI complex (3) RNase Ms*2'-GMP complex (4) RNase Ms*GfpC complex (5) Trypsin-SSI complex (6) Interferon-beta and (7) RNase Rh, using this改进系统。

项目成果

期刊论文数量(43)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
Yasuo Takeuchi: "Refined Crystal Strueture of the Complex of Subtilisin BPN' and Streptomyces Subtilisin Inhibitor at 1.8A^^° Resolution" J.Mol.Biol.(1991)
Yasuo Takeuchi:“枯草杆菌蛋白酶 BPN 和链霉菌枯草杆菌蛋白酶抑制剂复合物在 1.8A^^° 分辨率下的精制晶体结构”J.Mol.Biol.(1991)
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中村和郎: "結晶の分子科学入門(第5章第3節)" 講談社サイエンティフク, 236 (1989)
中村一夫:“晶体分子科学导论(第 5 章第 3 节)”讲谈社科学福,236(1989)
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中村 和郎: "タンパク質結晶構造の精密化における分子動力学の利用" 化学と工業. 43. 1286-1287 (1990)
Kazuo Nakamura:“利用分子动力学精炼蛋白质晶体结构”,化学与工业,43。1286-1287(1990)。
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    0
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Toshiya Senda: "Threeーdimensional Structure of Recombinant Murine Interferonーβ" Proceedings of the Japan Academy. 66. 77-80 (1990)
Toshiya Senda:“重组鼠干扰素-β 的三维结构”日本科学院院刊 66. 77-80 (1990)。
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    0
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Hiroyuki Kurihara: "Crystallization of a New Class of Microbial Ribonuclease from Rhizopus Niveus" J.Mol.Biol.206. 791-792 (1989)
Hiroyuki Kurihara:“来自尼维根霉的一类新微生物核糖核酸酶的结晶”J.Mol.Biol.206。
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共 39 条
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前往

NAKAMURA Kazuo其他文献

Improving Fast Ejecting System of Targeted Sample (FESTA) and Gas Calibration
改进目标样品快速喷射系统(FESTA)和气体校准
Exchangeability property of cluster structures
团簇结构的可交换性
  • DOI:
  • 发表时间:
    2020
    2020
  • 期刊:
  • 影响因子:
    0
  • 作者:
    KOJIMA Shinichiro;ELSERAFY Hatem;HANADA Kazuaki;IDEI Hiroshi;IKEZOE Ryuya;NAGASHIMA Yoshihiko;HASEGAWA Makoto;ONCHI Takumi;KURODA Kengoh;NAKAMURA Kazuo;MURAKAMI Takahiro;FUKUYAMA Masaharu;KATO Ryoya;YONEDA Ryota;ONO Masayuki;EJIRI Akira;TAKASE Yuichi;MURA;Asako Seki Hirashima;黒田卓; 近藤百世; 徳永佳晃;吉田航太;平島朝子;Gyoda Yasuaki
    KOJIMA Shinichiro;ELSERAFY Hatem;HANADA Kazuaki;IDEI Hiroshi;IKEZOE Ryuya;NAGASHIMA Yoshihiko;HASEGAWA Makoto;ONCHI Takumi;KURODA Kengoh;NAKAMURA Kazuo;MURAKAMI Takahiro;FUKUYAMA Masaharu;KATO Ryoya;YONEDA Ryota;ONO Masayuki;EJIRI Akira;TAKASE Yuichi;MURA;Asako Seki Hirashima;黒田卓; 近藤百世; 徳永佳晃;吉田航太;平島朝子;Gyoda Yasuaki
  • 通讯作者:
    Gyoda Yasuaki
    Gyoda Yasuaki
ダーク・インフラストラクチャー:インドネシア・スラバヤにおける廃棄物埋立処分場の不可視性について
黑暗基础设施:印度尼西亚泗水垃圾填埋场的隐形
  • DOI:
  • 发表时间:
    2020
    2020
  • 期刊:
  • 影响因子:
    0
  • 作者:
    KOJIMA Shinichiro;ELSERAFY Hatem;HANADA Kazuaki;IDEI Hiroshi;IKEZOE Ryuya;NAGASHIMA Yoshihiko;HASEGAWA Makoto;ONCHI Takumi;KURODA Kengoh;NAKAMURA Kazuo;MURAKAMI Takahiro;FUKUYAMA Masaharu;KATO Ryoya;YONEDA Ryota;ONO Masayuki;EJIRI Akira;TAKASE Yuichi;MURA;Asako Seki Hirashima;黒田卓; 近藤百世; 徳永佳晃;吉田航太
    KOJIMA Shinichiro;ELSERAFY Hatem;HANADA Kazuaki;IDEI Hiroshi;IKEZOE Ryuya;NAGASHIMA Yoshihiko;HASEGAWA Makoto;ONCHI Takumi;KURODA Kengoh;NAKAMURA Kazuo;MURAKAMI Takahiro;FUKUYAMA Masaharu;KATO Ryoya;YONEDA Ryota;ONO Masayuki;EJIRI Akira;TAKASE Yuichi;MURA;Asako Seki Hirashima;黒田卓; 近藤百世; 徳永佳晃;吉田航太
  • 通讯作者:
    吉田航太
    吉田航太
Quaternion Analysis of a Direct Matrix Converter Based on Space-Vector Modulation
基于空间矢量调制的直接矩阵变换器的四元数分析
  • DOI:
    10.1585/pfr.16.2405037
    10.1585/pfr.16.2405037
  • 发表时间:
    2021
    2021
  • 期刊:
  • 影响因子:
    0.8
  • 作者:
    NAKAMURA Kazuo;ZHANG Yifan;ONCHI Takumi;IDEI Hiroshi;HASEGAWA Makoto;TOKUNAGA Kazutoshi;HANADA Kazuaki;MITARAI Osamu;KAWASAKI Shoji;HIGASHIJIMA Aki;NAGATA Takahiro;SHIMABUKURO Shun
    NAKAMURA Kazuo;ZHANG Yifan;ONCHI Takumi;IDEI Hiroshi;HASEGAWA Makoto;TOKUNAGA Kazutoshi;HANADA Kazuaki;MITARAI Osamu;KAWASAKI Shoji;HIGASHIJIMA Aki;NAGATA Takahiro;SHIMABUKURO Shun
  • 通讯作者:
    SHIMABUKURO Shun
    SHIMABUKURO Shun
Initial testing of ohmic heating through double flux swing during electron cyclotron start-up in the QUEST spherical tokamak
在 QUEST 球形托卡马克电子回旋加速器启动期间通过双通量摆动进行欧姆加热的初步测试
  • DOI:
    10.1088/2058-6272/acafc2
    10.1088/2058-6272/acafc2
  • 发表时间:
    2023
    2023
  • 期刊:
  • 影响因子:
    1.7
  • 作者:
    ZHANG Yifan;ONCHI Takumi;NAKAMURA Kazuo;YUE Qilin;NAGATA Takahiro;KAWASAKI Shoji;KURODA Kengoh;HASEGAWA Makoto;IKEZOE Ryuya;IDO Takeshi;HANADA Kazuaki;IDEI Hiroshi
    ZHANG Yifan;ONCHI Takumi;NAKAMURA Kazuo;YUE Qilin;NAGATA Takahiro;KAWASAKI Shoji;KURODA Kengoh;HASEGAWA Makoto;IKEZOE Ryuya;IDO Takeshi;HANADA Kazuaki;IDEI Hiroshi
  • 通讯作者:
    IDEI Hiroshi
    IDEI Hiroshi
共 14 条
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前往

NAKAMURA Kazuo的其他基金

Investigation of the properties of a new natural cheese unique to Japan using the milk-clotting enzyme from Hericium erinaceum
使用猴头菇凝乳酶研究日本独有的新型天然奶酪的特性
  • 批准号:
    15K00780
    15K00780
  • 财政年份:
    2015
  • 资助金额:
    $ 2.94万
    $ 2.94万
  • 项目类别:
    Grant-in-Aid for Scientific Research (C)
    Grant-in-Aid for Scientific Research (C)
Sensorless Diamagnetic Measurement of Plasma Energy in Spherical Tokamak
球形托卡马克等离子体能量的无传感器抗磁测量
  • 批准号:
    25630422
    25630422
  • 财政年份:
    2013
  • 资助金额:
    $ 2.94万
    $ 2.94万
  • 项目类别:
    Grant-in-Aid for Challenging Exploratory Research
    Grant-in-Aid for Challenging Exploratory Research
Computing with Words for Understanding and Generation of Modal Human Motions affected by Mental and Physical Conditions
用文字计算来理解和生成受精神和身体状况影响的模态人体运动
  • 批准号:
    16500123
    16500123
  • 财政年份:
    2004
  • 资助金额:
    $ 2.94万
    $ 2.94万
  • 项目类别:
    Grant-in-Aid for Scientific Research (C)
    Grant-in-Aid for Scientific Research (C)
The reaction mechanism of the ribonuclease T2 family
核糖核酸酶T2家族的反应机制
  • 批准号:
    10672025
    10672025
  • 财政年份:
    1998
  • 资助金额:
    $ 2.94万
    $ 2.94万
  • 项目类别:
    Grant-in-Aid for Scientific Research (C)
    Grant-in-Aid for Scientific Research (C)
The Reaction Mechanism and Subsites of RNase T_2 family
RNase T_2家族的反应机制及亚位点
  • 批准号:
    08680663
    08680663
  • 财政年份:
    1996
  • 资助金额:
    $ 2.94万
    $ 2.94万
  • 项目类别:
    Grant-in-Aid for Scientific Research (C)
    Grant-in-Aid for Scientific Research (C)
REMOVABLE MAGNETIC KEEPER FREE FROM MRI ARTIFACT
可拆卸的磁性保持器,免受 MRI 伪影的影响
  • 批准号:
    06671946
    06671946
  • 财政年份:
    1994
  • 资助金额:
    $ 2.94万
    $ 2.94万
  • 项目类别:
    Grant-in-Aid for General Scientific Research (C)
    Grant-in-Aid for General Scientific Research (C)
The reaction mechanism and base recognition of RNase Rh
RNase Rh的反应机理及碱基识别
  • 批准号:
    06672154
    06672154
  • 财政年份:
    1994
  • 资助金额:
    $ 2.94万
    $ 2.94万
  • 项目类别:
    Grant-in-Aid for General Scientific Research (C)
    Grant-in-Aid for General Scientific Research (C)
Research on Disruption and Thermal Quench of Long-Pulse Discharge
长脉冲放电的破坏与热猝灭研究
  • 批准号:
    05680398
    05680398
  • 财政年份:
    1993
  • 资助金额:
    $ 2.94万
    $ 2.94万
  • 项目类别:
    Grant-in-Aid for General Scientific Research (C)
    Grant-in-Aid for General Scientific Research (C)
Transition of organization of living space and environmental view in Japan
日本居住空间组织与环境观的变迁
  • 批准号:
    61301087
    61301087
  • 财政年份:
    1986
  • 资助金额:
    $ 2.94万
    $ 2.94万
  • 项目类别:
    Grant-in-Aid for Co-operative Research (A)
    Grant-in-Aid for Co-operative Research (A)

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ChimeraX -- Next Generation Visualization and Analysis Software for Multiscale Modeling
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