Cell-Biological Study of Degenerative Structures (Rosenthal Fibers) in Gliomas.

神经胶质瘤退行性结构（罗森塔尔纤维）的细胞生物学研究。

基本信息

批准号：
01570825
负责人：
KOBAYASHI Shu
金额：
$ 1.34万
依托单位：
Tokyo Metropolitan Institute of Gerontology
依托单位国家：
日本
项目类别：
Grant-in-Aid for General Scientific Research (C)
财政年份：
1989
资助国家：
日本
起止时间：
1989 至 1990
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-01570825/
关键词：
Rosenthal fiber Glioma Degeneration Ubiquitin GFAP Crystallin

项目摘要

The aim of this project was to evaluate the degenerative process occuring in the cytoplasms of low-grade gliomas, such as Rosenthal fibers (RFs), and to clarify the biological characteristics of gliomas. Pertinent findings are described as below ; (1) Immunohistochemically we investigated RFs on specimen surgically removed from patients with qliomas (three cerebellar astrocytomas, three optic gliomas, two spinal cord astrocytomas, one spinal ganglioglioma).We utilized sections from the formalin-fixed paraffin-embedded tissues and stained them with anti-GFAP antibody (Ab) and two anti-ubiquitin Abs... anti-PHF (paired helical filament) monoclonal Ab (DF2) which recognizes ubiquitin and anti-ubiquitin polyconal Ab provided by Dr. Haas. RFs were eosinophilic (brightred on H&E), purply-stained with PTAH (metachromasia), black with Heidemhein's iron-hematoxylin, and negative with PAS. The peripheral parts of RFs were intensely stained with anti-GFAP. DF2 showed intense staining on the ring … More around negative central core of most RFs in every specimen. Anti-ubiquitin polyclonal Ab gave the similar results as DF2. From the study on the serial sections, the peripheral halo of RFs was positive with both anti-GFAP and anti-ubiquitin Abs.Both GFAP and ubiquitin were considered to exist in the periphery of RFs and they might play an important role to produce the amorphous core RFs. (2) We recognized granular bodies (Gb) in the cystoplasm of the glioma tissues among several cases described above, which are also considered to be degenerative change. Whole parts of Gbs were dark-blue on PTAH and positively stained with both GFAP and ubiquitin (a different finding from RFs). (3) We also showed the positive staining with GFAP and ubiguitin in the peripheral parts of RFs which appeared on the cyst wall of cerebellar hemangioblastoma in an autopsied case with massive cerebral hemorrhage. Recently, T. Iwaki reported that alphaB crystallin is the major constituent of RFs from the brain of Alexander disease. In progress is the investigation to evaluate what kind of role alphaB crystallin is playing in the glia and glioma cells by making a specific anti-alphaB crystallin antibody. Less

该项目的目的是评估低级别胶质瘤（例如罗森塔尔纤维（RF））细胞质中发生的退行性过程，并阐明胶质瘤的生物学特征，相关发现如下：（1）免疫组织化学研究。研究了从 Qliomas 患者手术切除的标本（三个小脑星形细胞瘤、三个视神经胶质瘤、两个脊髓星形细胞瘤、一个脊髓神经节胶质瘤）。我们利用福尔马林固定石蜡包埋组织的切片，并用抗 GFAP 抗体 (Ab) 和两种抗泛素抗体…抗 PHF（配对螺旋丝）单克隆抗体 (DF2) 进行染色，其中识别 Haas 博士提供的泛素和抗泛素多聚抗体呈嗜酸性（H&E 上呈亮红色），用 PTAH（异色症）染色，用海德姆海因铁苏木精染色，用 PAS 染色，RF 的外围部分用抗 GFAP 强烈染色，在大多数 RF 的阴性中心核心周围显示强烈染色。从连续切片的研究来看，抗泛素多克隆抗体的结果与 DF2 相似。抗 GFAP 和抗泛素抗体均呈阳性。GFAP 和泛素均被认为存在于 RF 的外围，并且它们可能在产生无定形核心 RF 中发挥重要作用（2）我们在中识别出颗粒体（Gb）。上述几例胶质瘤组织的胞浆也被认为是退行性改变，PTAH 上整个 Gbs 呈深蓝色，两者均呈阳性染色。 GFAP 和泛素（与 RFs 不同的发现） (3) 最近，我们在大面积脑出血的尸检病例中，在小脑血管母细胞瘤的囊壁上出现了 GFAP 和泛素的阳性染色。 T. Iwaki 报道称，αB 晶状体蛋白是亚历山大病大脑 RF 的主要成分，目前正在研究评估 αB 的作用。晶状体蛋白通过产生特异性抗 αB 晶状体蛋白抗体而在神经胶质细胞和神经胶质瘤细胞中发挥作用。