Natural Life Cycle of Gnathostoma Doloresi in Miyazaki Prefecture, with Emphasis on the Route of Infection to the Patients.

宫崎县多洛雷氏颚口虫的自然生命周期，重点是患者的感染途径。

基本信息

批准号：
01570217
负责人：
NAWA Yukifumi
金额：
$ 1.41万
依托单位：
Miyazaki Medical College
依托单位国家：
日本
项目类别：
Grant-in-Aid for General Scientific Research (C)
财政年份：
1989
资助国家：
日本
起止时间：
1989 至 1990
项目状态：
已结题

项目摘要

In 1988, we found the first confirmed human case of Gnathostoma doloresi infection in Miyazaki Prefecture. In addition, two more confirmed cases were found by the retrospective survey of paraffin-embedded biopsied skin of 7 suspected cases. Up to now, a total of 14 human gnathostomiasis doloresi cases including 4 confirmed cases were found in the central part of Miyazaki Prefecture. Among these 14 cases, 13 cases were anglers or their wife having a common past history of eating raw slice meat of freshwater fishes, mainly Oncorhynchus masou. One case had a past history of eating raw slice meat meat of a snake, Agkistrodon halys. Based on the history of the patients, we conducted a survey to elucidate the natural life cycle of G. doloresi and to identify the source of infection to the patients. The wild boars, Sus scrofa leucomystax, captured in the mountainous areas of Miyazaki Pref. Were infected with G. doloresi with 100% incidence. Artificially hatched L_1 from the intrauterine eggs … More from the adult female worms could grew up into L_3 in the first intermediate host. cold water cyclops. As the second intermediate host and/or the paratenic host, the poisonous snakes, Agkistrodon halys, captured in the mountainous areas of Miyazaki Pref. were infected with G. doloresi L_3 with 100% incidence. The L_3 recovered from A. halys could develop into mature adult worms by an experimental infection in a pig. In addition, a nonpoisonous snake, Elaphe quadrivergata, also harbored G. doloresi L_3. These results suggest that snakes are important in the natural life cycle of this parasite. From the anamnes is of the patients, freshwater fishes are most likely source of infection to the patients. Therefore, we examined two kinds of freshwater fishes, common Japanese name "Yamame", Oncorhynchus masou, and common name "Blue gill" , Lepomis macrochirus, both captured at Hitotsuse River. Although more than 200 each were examined, G. doloresi larvae were not yet found from these fishes. Further extensive survey is necessary for the identification of the source of infection. Less

1988年，宫崎县发现首例人类感染颚口线虫感染病例，此外，对7例疑似病例进行石蜡包埋活检皮肤回顾性调查，又发现2例确诊病例，目前共计14例。宫崎县中部发现人类多洛雷丝虫病病例，其中确诊病例4例，其中14例。 13 例钓鱼者或其妻子有共同食用淡水鱼生片肉的历史，其中 1 例有生吃蛇片肉的历史。我们对患者进行了一项调查，以阐明 G. doloresi 的自然生命周期，并确定捕获的野猪 Sus scrofa leucomystax 的感染源。宫崎县山区感染G. doloresi的几率为100%，从宫内卵中人工孵出的L_1成虫可在冷水独眼虫中成长为L_3。第二中间宿主和/或副宿主，在宫崎县山区捕获的毒蛇Agkistrodon halys被感染。 G. doloresi L_3 的发生率为 100%。从 A. halys 中回收的 L_3 可以通过在猪中进行实验感染而发育成成熟的成虫。此外，无毒蛇 Elaphe quadrivergata 也含有 G. doloresi L_3。表明蛇在这种寄生虫的自然生命周期中很重要，从患者的病史来看，淡水鱼最有可能是患者的感染源。因此，我们检查了两种淡水鱼，日本通用名“Yamame”，Oncorhynchus masou，和通用名“Blue gill”，Lepomis Macrochirus，两者都是在一濑川捕获的，虽然每种都检测了 200 多条，但 G. doloresi 幼虫却被检测到。尚未从这些鱼类中发现，需要进一步广泛调查以确定感染源。