Seroepidemiological study on Ascaris suum infection in humans, an emerging zoonosis causing visceral larva migrans

人类猪蛔虫感染的血清流行病学研究，一种引起内脏幼虫移行症的新出现的人畜共患病

• 批准号: 09470070
• 负责人: NAWA Yukifumi
• 金额: $ 7.49万
• 依托单位: Miyazaki Medical College
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 1997
• 资助国家: 日本
• 起止时间: 1997 至 1999
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-09470070/
• 关键词: Ascaris suum; visceral larva migrans; zoonosis; pigs; epidemiology; immunodiagnosis; allergy; pollinosis; 幼虫移行症; 新興感染症; 好酸球; 疫学調査; 免疫血清診断; 人獣共通寄生虫症; 土壌媒介寄生虫症; 食品媒介寄生虫症; 新興・再興感染症; IgE

Ascaris suum is naturally an intestinal parasite of pigs. Viscerallarva migrans (VLM) characterized as pneumonitis and/or liver dysfunction with marked eosinophilia has been known to occur after experimental or accidental ingestion of A. suum embryonated eggs. However, natural occurrence of VLM due to A. suum has never been reported until our recent discovery of the presence of this disease in the pig-farming area in Kyushu. By the end of 1999, we have found 128 cases of immunoserologically diagnosed VLM probably caused by infection with A. suum. These cases included 2 ocular and 5 CNS involvement. After the informed concent has obtained, seroepidemiological survey was conducted in the pig-farming area in Kyushu; 6 towns in Miyazaki Prefecture and 2 towns in Kagoshima prefecture. The results show that 20-30% of the residents living in pig-farming area were seropositive against A. suum antigen by IgG-ELISA, while those living in non-pig-farming area showed less than 10% seropositivity against A. suum. Because parasitic infection has been supposed to affect and modulate allergic status, we examined the relationship between A. suum infection and allergy in the pig-farming area in Kyushu. For this purpose, specific IgE antibody against cedar pollen and/or mite allergen was measured as well as specific IgG/IgE antibodies against A. suum. The results show that the prevalence of allergic individuals having anti-cedar pollen and/or anti-mite IgE antibody was far greater in A. suum-infected group compared to that in parasite-free group. These results suggest that parasite infection is one of the important risk factor for allergic disease.

猪蛔虫是猪的天然肠道寄生虫。实验性或意外摄入猪蛔虫胚卵后，已知会发生内脏幼虫移行症 (VLM)，其特征为肺炎和/或肝功能障碍并伴有明显的嗜酸性粒细胞增多。然而，直到我们最近在九州养猪区发现这种疾病的存在之前，从未报道过由猪曲霉引起的 VLM 的自然发生。到1999年底，我们已发现128例经免疫血清学诊断可能由猪曲霉感染引起的VLM。这些病例包括 2 例眼部受累和 5 例中枢神经系统受累。掌握通报情况后，对九州地区生猪养殖区进行了血清流行病学调查；宫崎县6町、鹿儿岛县2町。结果显示，生猪养殖区居民中20%～30%的猪曲霉抗原血清IgG-ELISA阳性，而非养猪区居民猪曲霉血清阳性率不足10%。由于寄生虫感染被认为会影响和调节过敏状态，因此我们研究了九州养猪地区猪曲霉感染与过敏之间的关系。为此，测量了针对雪松花粉和/或螨过敏原的特异性 IgE 抗体以及针对猪曲霉的特异性 IgG/IgE 抗体。结果表明，与无寄生虫组相比，猪曲霉感染组中具有抗雪松花粉和/或抗螨IgE抗体的过敏个体的患病率要高得多。这些结果表明寄生虫感染是过敏性疾病的重要危险因素之一。

项目成果

名和行文: "寄生虫疾患とアレルギーの合併"アレルギーの臨床. 19. 40-44 (1999)

Yukibumi Nawa：“寄生虫病和过敏的并发症”过敏临床。 19. 40-44 (1999)

名和行文: "寄生虫とアレルギーの関係"臨床免疫. 33. 202-207 (2000)

Yukibumi Nawa：“寄生虫与过敏之间的关系”临床免疫学 33. 202-207 (2000)。

Maruyama H, Noda S, Choi W-Y, Ohto N, Nawa Y: "Fine blinding specificities to Ascaris lumbricoides antigens of the sera from patients of probable visceral larva migrans due to Ascaris suum"Parasitol. International.. 46. 181-188 (1997)

Maruyama H、Noda S、Choi W-Y、Ohto N、Nawa Y：“对可能由猪蛔虫引起的内脏幼虫移行症患者血清中的蛔虫抗原具有良好的致盲特异性”Parasitol。

Y.Inatomi.,T.Murakami.,et al.: "Encephalopathy caused by visceral larva migrans due to Ascaris suum"Journal of the Neurological Sciences. 164. 195-199 (1999)

Y.Inatomi.，T.Murakami.，et al.：“猪蛔虫引起的内脏幼虫移行性脑病”神经科学杂志。

松下隆司、田原良博、山本章二郎、永田賢治、駒田真人、堀剛、井戸章雄、弘野修一、林克裕、坪内博仁、名和行文: "肝内に多発性結節病変を認めたブタ蛔虫による内臓幼虫移行症の一例"肝臓. 38. 730-734 (1997)

Takashi Matsushita、Yoshihiro Tahara、Shojiro Yamamoto、Kenji Nagata、Masato Komada、Tsuyoshi Hori、Akio Ido、Shuichi Hirono、Katsuhiro Hayashi、Hirohito Tsubouchi、Yukifumi Nawa：“由猪蠕虫引起的肝脏内有多个结节性病变的内部幼虫。一个例子过渡性疾病“Liver. 38. 730-734 (1997)