Development of Novel Synthetic Bilayer System Possessing Chemical Information Transduction and Amplfication Ability

具有化学信息传导和放大能力的新型合成双层系统的开发

基本信息

批准号：
01550579
负责人：
NAKASHIMA Naotoshi
金额：
$ 1.47万
依托单位：
Nagasaki University
依托单位国家：
日本
项目类别：
Grant-in-Aid for General Scientific Research (C)
财政年份：
1989
资助国家：
日本
起止时间：
1989 至 1990
项目状态：
已结题

项目摘要

Biological membranes are composed of lipid bilayer and membrane proteins and act as transducers which convert chemical signals to electrical signals. The aim/purpose of this study is to develop novel molecular recognition and information transduction systems mimic to biological membranes. The summaries are as follows.(1) A mercaptan-containing phosphate lipid was designed and prepared. Monolayer of the lipid was fabricated on gold electrodes via chemisorption from ethanol and their properties were examined by cyclic voltametry. The permeation or diffusion of ferricyanide and ferrocene ammonium into the monolayer on the electrode could be controlled by changing pH of the solution. The electrodes were found to response to alkali earth metal ions.(2) Fundamental examinations on design and fabrication of bilayer devices which transduce molecular information to electric or electrochemical signals were conducted. Quaternary ammonium lipids on patterned electrodes showed drastic change in impedance and capacitance upon heating above phase transition of bilayers. However, primary ammonium lipids exhibited no such phase transition dependence. Despite the very small transition enthalpy, single chain quaternary ammonium film showed the same Tc dependence in impedance measurements as those with double chain lipids. Impedance characteristics of biological lipid films were also investigated. Molecular mechanisms for the obtained results were analyzed by cole-cole plot analyses of impedance.

生物膜由脂质双层和膜蛋白组成，充当将化学信号转换为电信号的传感器。本研究的目的/目的是开发模仿生物膜的新型分子识别和信息转导系统。主要研究内容如下： (1)设计并制备了含硫醇的磷酸酯。通过乙醇的化学吸附在金电极上制备单层脂质，并通过循环伏安法检查其性质。铁氰化物和二茂铁铵渗透或扩散到电极上的单层中可以通过改变溶液的pH值来控制。发现该电极对碱土金属离子有响应。(2)对将分子信息转换为电或电化学信号的双层器件的设计和制造进行了基础研究。图案化电极上的季铵脂质在加热至双层相变以上时表现出阻抗和电容的剧烈变化。然而，伯铵脂质没有表现出这种相变依赖性。尽管转变焓非常小，但单链季铵薄膜在阻抗测量中表现出与双链脂质相同的 Tc 依赖性。还研究了生物脂质膜的阻抗特性。通过阻抗的科尔-科尔图分析来分析所获得结果的分子机制。