Adaptations to Environmental Pressure: The Effect of Pressure on the Properties and Functions of Model Biomembranes.

对环境压力的适应：压力对模型生物膜特性和功能的影响。

• 批准号: 02455015
• 负责人: KANESHINA Shoji
• 金额: $ 4.03万
• 依托单位: The University of Tokushima
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (B)
• 财政年份: 1990
• 资助国家: 日本
• 起止时间: 1990 至 1992
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-02455015/
• 关键词: Liposome; Bilayer; Phase transition; High pressure; Micelle; Ion-selective electrode; Local anesthetics; Mitochondria; リポソ-ム; ベシクル; 吸入麻酔薬; 圧拮抗

1. Phospholipid bilayers of dipalmitoylphosphatidylcholine (DPPC) have been used as a model for biomembranes. The temperature-pressure phase diagrams of DPPC multilamellar vesicles were constructed, which included four states, i.e., liquid crystal, ripple gel, lamellar gel and interdigitated gel phases. The interdigitated gel phase was induced by pressure and also by the addition of ethanol. Pressure facilitates, rather than antagonizes, the effect of ethanol on the occurrence of interdigitated gel phase in lipid bilayers.2. The mitochondrial functions (oxygen consumption and ATP synthesis) were used as a measure of adaptation to biological environment. Potentiometry using ion-selective electrode sensitive to lipid-soluble cations such as tetraphenylphosphonium (TPP) ion as a probe is applicable to the estimation of the membrane potential of mitochondria.3. Coated wire electrodes sensitive to local anesthetic cations were developed, which showed linear responses with Nernstian slopes over a concentration range of 10^<-5> - 10^<-2> M. These electrodes are available for measurement of the local anesthetic concentration under high pressure and in vivo.4. Surface tension, density and DSC of mixed surfactant solutions were measured. Miscibility in micelles and the changes of volume, enthalpy and entropy of mixing were discussed thermodynamically.

1. 二棕榈酰磷脂酰胆碱 (DPPC) 的磷脂双层已被用作生物膜的模型。构建了DPPC多层囊泡的温度-压力相图，包括液晶相、波纹凝胶相、层状凝胶相和叉指状凝胶相四种状态。通过压力以及添加乙醇诱导交叉指状凝胶相。压力促进而不是拮抗乙醇对脂质双层中交叉凝胶相的影响。2．线粒体功能（耗氧量和 ATP 合成）被用作适应生物环境的指标。采用对脂溶性阳离子如四苯基鏻(TPP)离子敏感的离子选择电极作为探针的电位测定法适用于线粒体膜电位的估计。 3．开发了对局麻药阳离子敏感的涂层线电极，其在 10^<-5> - 10^<-2> M 的浓度范围内显示出能斯特斜率的线性响应。这些电极可用于测量高压和体内。4．测量了混合表面活性剂溶液的表面张力、密度和DSC。从热力学角度讨论了胶束的混溶性以及混合的体积、热函和熵的变化。

项目成果

Shoji Kaneshina: "Functions of Model Biomembranes under High Pressure" J. Soc. Mat. Sci. Japan. 41. 336-341 (1992)

Shoji Kaneshina：“模型生物膜在高压下的功能”J. Soc。

KANESHINA Shoji,TAMURA Katsuhiro,KAWAKAMI Hitoshi,MATSUKI Hitoshi: "Effects of pressure and ethanol on the phase behavior of dipalmitoylphosphatidylcholine multilamellar vesicles." Chem.Lett.1992. 1963-1966 (1992)

KANESHINA Shoji、TAMURA Katsuhiro、KAWAKAMI Hitoshi、MATSUKI Hitoshi：“压力和乙醇对二棕榈酰磷脂酰胆碱多层囊泡相行为的影响。”

Hiroshi Terada: "Action Mechanism of Anesthetics on Biomembranes" Protein, Nucleic Acid, and Enzyme. 36. 1770-1778 (1991)

寺田浩：“麻醉剂对生物膜的作用机制”蛋白质、核酸和酶。

SATAKE Hiromu;MATSUKI Hitoshi;KANESHINA Shoji: "Colloidal Properties of Aqueous Local Anesthetic Tetracaine Solutions" Colloids and Surfaces.

SATAKE Hiromu；MATSUKI Hitoshi；KANESHINA Shoji：“局部麻醉丁卡因水溶液的胶体特性”胶体和表面。

Hiromu Satake: "Coated Wire Electrodes Sensitive to Local Anesthetic Cations and Their Application to Potentiometric Determination" Bull. Chem. Soc. Japan. 64. 3029-3034 (1991)

Hiromu Satake：“对局部麻醉阳离子敏感的涂层线电极及其在电位测定中的应用”公牛。