HLA-DR Antigen Positive Cells in the RA Synovium. -Their Significant Role Concerning in the Continuation of Chronic Inflammation of RA.

RA 滑膜中的 HLA-DR 抗原阳性细胞。

• 批准号: 63570138
• 负责人: MURAKAMI Kazuhiro
• 金额: $ 1.41万
• 依托单位: TOHOKU UNIVERSITY
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (C)
• 财政年份: 1988
• 资助国家: 日本
• 起止时间: 1988 至 1990
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-63570138/
• 关键词: Rheumatoid arthritis; Synovial cell; HLA-DR antigen; 清膜細胞; リウマトイド結節; HLAーDR陽性細胞; HLA; DR抗原; 螢光抗体法

1 Characteristics of RA synovial cells The sublining D cells which were remarkably proliferating in the rheumatoid synovial membrane were divided into two type cells, D(F) cells and D(M) cells immunohistochemically. In culture, D(F) cells showed high proliferative activity and easily transformed their morphological and functional character under the effects of variable cytokines. On the other hand, D(M) cells proliferated very slowly, and they contiguously and strongly expressed HLA-DR antigen on their surface. These findings suggest that D(F) cell plays an important role in formation of histopathological variety of RA synovitis and D(M) cell is largely concerned in the immunological activity of RA. 2 Histopathological study of early stage of synovial lesion We investigated the early stage of RA synovitis immunohistochemically and from this study it become clear that in very early of RA, HLA-DR positive cells were found in pericapillary region and following them T lymphocytes appeared and B lymphocytes appeared to aggregate in later. 3 The effects of drugs on RA synovitis Some patients with early stage RA showed improvement of condition of disease after treatment with immunomodulating drug (CCA), but no apparent qualitative and quantitative changes of HLA-DR positive cells in synovium were observed. These findings suggest the complexity of cellular interaction in development of RA synovitis. 4 Comparison of synovial cells with cells in rheumatoid nodule The characteristics of majority of cells in rheumatoid nodule were quite similar to that of D cells in rheumatoid synovitis. Clinically rheumatoid synovitis is characterized by its progressive and destructive nature in contrast to rheumatoid nodule. Histopathologically in the rheumatoid nodule the lymphocyte infiltration was less conspicuous. These findings suggest that lymphocyte infiltration is essential for the continuation of chronic destructive inflammation of RA synovitis.

1 RA滑膜细胞的特征将在类风湿滑膜中显着增殖的升华D细胞分为两个类型的细胞D（F）细胞和D（M）细胞免疫组织化学。在培养物中，D（F）细胞在可变细胞因子的作用下显示出较高的增殖活性，并很容易转化其形态和功能特征。另一方面，D（M）细胞非常缓慢增殖，它们在表面上连续且强烈表达HLA-DR抗原。这些发现表明，D（f）细胞在RA滑膜炎的组织病理学品种和D（M）细胞的形成中起重要作用，这在很大程度上与RA的免疫学活性有关。 2对滑膜病变的早期阶段的组织病理学研究我们在从本研究上研究了RA滑膜炎免疫组织化学的早期阶段，很明显，在RA的早期，在乳腺污染区域中发现了HLA-DR阳性细胞，并且在后来出现了淋巴细胞，而淋巴细胞似乎在后来聚集。 3药物对RA滑膜炎的作用一些早期RA患者在治疗免疫调节药物（CCA）治疗后的疾病状况改善，但没有观察到Synovium中HLA-DR阳性细胞的明显定性和定量变化。这些发现表明RA滑膜炎发育中细胞相互作用的复杂性。 4滑膜细胞与类风湿结节中细胞的比较。类风湿结节中大多数细胞的特征与类风湿滑膜炎中D细胞的特征非常相似。与类风湿结节相比，临床上类风湿滑膜炎的特征是其进行性和破坏性性质。在类风湿结节中，组织病理学淋巴细胞浸润的明显不太明显。这些发现表明，淋巴细胞浸润对于继续慢性破坏性炎症至关重要。

项目成果

沢井 高志: "慢性関節リウマチ滑膜初期病変の免疫組識学的検討 ーモノクロナル抗体を用いた炎症性細胞の定性ならびに定量的解折ー" リウマチ. 30. 247-254 (1990)

Takashi Sawai：“类风湿性关节炎早期滑膜病变的免疫组织学研究 - 使用单克隆抗体对炎症细胞进行定性和定量分析”Rheumatology 30. 247-254 (1990)。

Sawai T, Murakami K, et al.: "Stromelysin synthesizing cells in the synovial tissue of rheumatoid arthritis demonstrated by in situ hybridization and immunohistochemical methods" Tohoku J. Exp. Med. 160. 285-286 (1990)

Sawai T、Murakami K 等人：“通过原位杂交和免疫组织化学方法证明类风湿性关节炎滑膜组织中溶质素合成细胞”Tohoku J. Exp。

沢井 高志: "慢性関節リウマチ最近の話題ーMetalloproteinaseとマトリックスの観点からー" 病理と臨床. 8. 1478-1484 (1990)

Takashi Sawai：“类风湿性关节炎的当前主题 - 从金属蛋白酶和基质的角度”病理学和临床研究 8. 1478-1484 (1990)。

Murakami Kazuhiro: "Morphological and functional characteristics of cultured human synovial cells obtained from rheumatoid synovium"

Murakami Kazuhiro：“从类风湿滑膜中获得的培养人类滑膜细胞的形态和功能特征”

村上一宏他: "臨床VisnalMook〓慢性関節リウマチ〓" 全日本病院出版会,

Kazuhiro Murakami等人：《临床视觉Mook〓慢性类风湿性关节炎〓》全日本医院出版协会，