Systematic Studies on Host and Endosymbiont of a Green Dinoflagellate

绿色甲藻宿主与内共生体的系统研究

• 批准号: 63540554
• 负责人: WATANABE Makoto M.
• 金额: $ 1.47万
• 依托单位: National Institute for Environmental Studies
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (C)
• 财政年份: 1988
• 资助国家: 日本
• 起止时间: 1988 至 1990
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-63540554/
• 关键词: Green dinoflagellate; Lepidodinium viride; Taxonomy; Ultrastructure; Body scale; Endosymbiont; Carotenoid; Prasinophyceae; 鱗片; 共生体; ペリクル層; 色素; 共生緑色渦鞭毛藻; Lepidodinium viridis; HPLC

A further description of the green dinoflagellate, strain Y-100, with a chlorophyll a- and b- containing endosymbiont is given with special emphasis on the morphology of the host cell. The host dinoflagellate cell is unarmored and has a gymnodinioid overall appearance. The theca or amphiesma basically consists of the outer membrane and flattened thecal vesicles in which no thecal plates are developed. Unusual hand basket-shaped scales cover the entire cell surface together with a layer of mucillaginous material. These findings suggest that the organism is a new member of the Gymnodiniaceae (Gymnodiniales) ; We propoes the name Lepidodinium viride gen. et sp. nov. The ultrastructure of the host cell is basically typical of the dinoflagellates. However, the organism has 1) an usual cytoplasmic projection that may be a homologue of the peduncle, 2) a single membrane-bounded body containing membranous sheets, closely situated next to the endosymbiont, and 3) an electron opaque network-forming appendage surrounding the transverse flagellum. None of these features have been found in other dinoflagellates.Major carotenoids of a endosymbiont in a green dionflagellate, Lepidodinium viride have been analyzed using high-performance liquid chromatography (HPLC). The endosymbiont contains the usual chlorophytes carotenoids, that are neoxanthin, violaxanthin, antheraxanthin and beta-carotene. In addition to thses carotenoids, the two unknown peaks were detected. One peak of the tow is situated at the identical retention time to that of lutein, however, the absorption spectra is diffrent from that of lutein obtained from spinach leaves. Another peak cannot be identified becaues it has quite low absorbance, but seems to be identical to that of zeaxanthin. Based on these results, it is speculated that a prasinophyte would seem to be likeliest candidate for a proqenitor of the endosymbiont.

对绿色鞭毛盐Y-100的进一步描述，具有叶绿素A和含有B-含有内共生的叶绿素，特别强调了宿主细胞的形态。宿主的鞭毛藻细胞没有武装，并且具有锻炼整体外观。塞子或两栖动物基本上是由外膜组成的，并扁平的胸小囊泡，其中未开发thecal板。不寻常的手篮形鳞片与一层粘液材料一起覆盖整个细胞表面。这些发现表明该生物是Gymnodiniaceae（Gymnodiniales）的新成员。我们提出了鳞翅目Viride Gen的名称。 et sp。十一月。宿主细胞的超微结构基本上是鞭毛藻的典型代表。然而，该生物具有1）通常是花梗的同源物的通常的细胞质投影，2）一个包含膜片的单个膜结合的身体，紧密位于内共生膜旁边，以及3）一个电子不透明的网络构造的网络围绕横向鞭毛的网络附属物。这些特征在其他鞭毛藻中都没有找到。在绿色二氟酸酯酸酯中的内共生型类胡萝卜素，已经使用高性能液相色谱（HPLC）分析了鳞翅目Viride。内共生体包含通常的叶绿素类胡萝卜素，这些类胡萝卜素是新氧蛋白酶，abityaxanthin，amtheraxanthin和beta-碳纤维素。除了类胡萝卜素外，还检测到了两个未知峰。拖曳的一个峰位于与叶黄素相同的保留时间，但是，吸收光谱与从菠菜叶获得的叶黄素相差。无法识别另一个峰值，因为它的吸光度很低，但似乎与Zeaxanthin相同。基于这些结果，据推测，对于内共生菌的培养基似乎是最有可能的候选者。

项目成果

Watanabe et al.,: "Major carotenoid of a chlorophyll aーand bーcontaining endosymbiont in a green dinoflagellnte,Lepidodinium viride (Gymnodiniales,Dinophyta)." Jpn J.plycol.

Watanabe 等人：“绿色甲藻、绿色鳞甲藻（Gymnodiniales，Dinophyta）中含有叶绿素 a 和 b 的内共生体的主要类胡萝卜素。”

Watanabe, M. M., Suda, S., Sasa, T., Inouye, I. & Chihara, M.: "Major carotenodids of a chlorophyll a- and b-containing endosymbiont in a green dinoflagellate, Lepidodinium viride (Gymnodini" Jpn. J. Phycol.

渡边，M.M.，须田，S.，莎莎，T.，井上，I.

Suda,S.,Watanabe,M,M.& Inouye,I: "Evidence for sexual reproduction in the primitive green alga Nephroselmis olivacea (Prasinophyceae)" J.Phycol. 25. 596-600 (1989)

须田，S.，渡边，M，M。

Watanabe, M. M., Suda, S., Inouye, I., Sawaguchi, T. & Chihara, M.: "Lepidodinium viride gen. et sp. nov (Gymnodiniales, Dinophyta), a green dinoflagellate with a chlorophyll a- and b-containing" J. Phycol. 26. 741-751 (1990)

渡边，M.M.，须田，S.，井上，I.，泽口，T.

Suda, S., Watanabe, M. M. & Inouye, I.: "Evidence for sexual reproduction in the primitive green alga Nephroselmis olivacea (Prasinophyceae)." J. Phycol. 25. 596-600 (1989)

须田，S.，渡边，M.M.